Lecture 3 Flashcards
What is the holistic approach in systems biology?
The all at once approach; required as some properties of a system can emerge from the interaction of components
What is meant by “Capturing the Proteome”
Identifying the total proteins of a cell at a single point in time (which is thusfar technically challenging)
List 3 clinical implications of bioinformatics
Diagnosis of genetic disease and disease risk
Pharmacogenomics; predicting patient response - personalised medicine
Gene therepy, where thereputic target is a specific gene
What is homology modelling?
Homologous structures may have similar structures
This means an atomic level model of a target protein can be made from its primary sequence and experimental 3D data from a related homologous protein, the template
How is the potential proteome determined?
Inferred from genome sequence
What is a feature of amino acids with chemically similar side chains?
They substitute more readily
What is the reductionist approach in systems biology?
The breaking down (gene knockouts to disrupt biochemical pathways) of a biological system to understand the individual components
What is the primary database for protein structures?
wwPDB (World Wide Protein Data Bank)
What is meant by “Transcriptome”?
The transcriptome encompases the actual proteome (Translation is dependent on transcription)
What are 3 data resources for proteomics?
Genome sequences
Transcriptomes (microarray/RNAseq)
Protein Mass Spectrometry
