lecture 3 - craggs Flashcards
1
Q
quantum yield equation
A
number of photons emitted / number of photons absorbed
2
Q
beer lambert law
A
A = E c l
E molar extinction coefficient
c concentration
l path length
3
Q
brightness equation
A
E phi
molar extinction coefficient
quantum yield
4
Q
what does S65T do
A
disrupts the h-bond network
destabilising neutral chromophore
5
Q
amino acids you can change to change the colour of GFP
A
ser65
tyr66
gly67
6
Q
how to red shift spectra
A
pi - pi stacking
increased polarizability
7
Q
why does evolution optimise flourescence
A
using a selection method
8
Q
how to use FPs as sensors
A
test solutions because they are sensitive to pH and chloride calcium and pressure
