Lecture 3 Safety in the Clinical Lab

Question 1

Routine practice includes

Answer 1

• hand hygiene
• working surface disinfection
• use of PPE
• administrative controls
• environmental controls
• risk assessment

Question 2

older names for routine practices

Answer 2

standard precautions, universal precautions

Question 3

PPE is used for

Answer 3

infection control

Question 4

disinfection

Answer 4

destroys many or all pathogenic microorganism. does not kill sports. means of decontamination

Question 5

what is presept

Answer 5

sodium dichlorosocyanurate

Question 6

what is phenokil

Answer 6

phenolic based germicide

Question 7

decontamination

Answer 7

achieved through autoclave, chemical disinfection and inception. kills microorganism

Question 8

diff btw disinfection and decontamination

Answer 8

disinfect things that might be contaminated. decontaminate things known to be contaminated

Question 9

sterilization

Answer 9

kills all bacteria and spores. use of autoclave

Question 10

autoclave parameters

Answer 10

121 Celcius, 15 psi, 30-60 mins

Question 11

biological risk groups is about

Answer 11

the organism

Question 12

biological containment level is about

Answer 12

the environment in which you work with the org

Question 13

Risk group 1

Answer 13

low individual risk and low community risk

Question 14

risk group 2

Answer 14

moderate individual risk and low community risk

Question 15

risk group 3

Answer 15

high individual risk and low community risk

Question 16

risk group 4

Answer 16

high individual and high individual risk

Question 17

e.g. of risk group 1

Answer 17

normal flora, lactobacillus

Question 18

e.g. of risk group 2

Answer 18

clostridium, hepatitis, influenza strains

Question 19

e.g. of risk group 3

Answer 19

HIV, TB

Question 20

e.g. of risk group 4

Answer 20

ebola virus, variola virus

Question 21

physical requirements for level 1 biological contamination

Answer 21

open bench top, use of safe practices and PPE

Question 22

physical requirements for level 2 biological contamination

Answer 22

BSC, PPE, autoclaves, use of dafe practices and appropriate PPE. most clinical labs

Question 23

physical requirements for level 3 biological contamination

Answer 23

access control, HEPA filtered exhaust, respiratory protection, safe practices, PPE

Question 24

physical requirements for level 4 biological contamination

Answer 24

fully seals, worked wear +ive pressure suits, class III BSC, decontamination of exhaust and wastes

Question 25

BSC classification is based on

Answer 25

cabinet's protection for the workers, product and environment

Question 26

what determines the classification of BSC

Answer 26

roomair, contaminated air and filtered air flow patterns

Question 27

list the classes and types for BSC

Answer 27

Class I Class II Type A1, Class II Type A2 Class II Type B1, Class II Type B2 Class III

Question 28

Describe HEPA filters

Answer 28

removed 99.99% airborne particles 0.3 um or greater. delicate construction. tested and certified for use in clinical labs

Question 29

Describe Class 1 BSC

Answer 29

uncirculated air flows away from operator passes through HEPA & is discharged into atmosphere. operator protection. no product protection

Question 30

Describe Class II BSC

Answer 30

most common. for working with microorganism in containment L 2,3,4 lab. other personal, product and environment protection.

Question 31

how care Class II Type A (1and 2) and B (1 and 2) differentiated

Answer 31

by their construction, airflow velocity, airflow pattern and exhaust system

Question 32

describe Class II type A1

Answer 32

cabinet air is either recirculated into lab or ducted out building by thimble connection after passing HEPA. not suitable for volatile chemicals or radionuclides. avg face velocity 0.38m/s. may have +ive pressure contaminated ducts

Question 33

describe Class II type A2

Answer 33

cabinet air is either recirculated into lab or ducted out building by thimble connection after passing HEPA. suitable for low levels volatile toxic chemicals and radionuclides. avg face velocity of 0.5 mms. ducts and plenums under negative pressure

Question 34

Describe Class II, Type B1

Answer 34

hard ducted. Exhaust passes through HEPA to atmosphere. suitable for low levels volatile chemical or radionuclides. contains -ive pressure plenums. min face velocity 0.5 m/s

Question 35

Describe Class II, Type B2

Answer 35

hard ducted. exhausts 100% of cabinet air thru HEPA to atmosphere. contains -ive pressure plenums. face velocity 0.5m/s

Question 36

Describe Class III

Answer 36

full air tight enclosure. HEPA filtered supply and exhaust air. cabinet is negative pressure work performed thru long sleeve gloves. protection for worker and product. good for group 4 organisms