Lecture 3 Safety in the Clinical Lab Flashcards Preview

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Flashcards in Lecture 3 Safety in the Clinical Lab Deck (36):
1

Routine practice includes

-hand hygiene
-working surface disinfection
-use of PPE
-administrative controls
-environmental controls
-risk assessment

2

older names for routine practices

standard precautions, universal precautions

3

PPE is used for

infection control

4

disinfection

destroys many or all pathogenic microorganism. does not kill sports. means of decontamination

5

what is presept

sodium dichlorosocyanurate

6

what is phenokil

phenolic based germicide

7

decontamination

achieved through autoclave, chemical disinfection and inception. kills microorganism

8

diff btw disinfection and decontamination

disinfect things that might be contaminated. decontaminate things known to be contaminated

9

sterilization

kills all bacteria and spores. use of autoclave

10

autoclave parameters

121 Celcius, 15 psi, 30-60 mins

11

biological risk groups is about

the organism

12

biological containment level is about

the environment in which you work with the org

13

Risk group 1

low individual risk and low community risk

14

risk group 2

moderate individual risk and low community risk

15

risk group 3

high individual risk and low community risk

16

risk group 4

high individual and high individual risk

17

e.g. of risk group 1

normal flora, lactobacillus

18

e.g. of risk group 2

clostridium, hepatitis, influenza strains

19

e.g. of risk group 3

HIV, TB

20

e.g. of risk group 4

ebola virus, variola virus

21

physical requirements for level 1 biological contamination

open bench top, use of safe practices and PPE

22

physical requirements for level 2 biological contamination

BSC, PPE, autoclaves, use of dafe practices and appropriate PPE. most clinical labs

23

physical requirements for level 3 biological contamination

access control, HEPA filtered exhaust, respiratory protection, safe practices, PPE

24

physical requirements for level 4 biological contamination

fully seals, worked wear +ive pressure suits, class III BSC, decontamination of exhaust and wastes

25

BSC classification is based on

cabinet's protection for the workers, product and environment

26

what determines the classification of BSC

roomair, contaminated air and filtered air flow patterns

27

list the classes and types for BSC

Class I
Class II Type A1, Class II Type A2
Class II Type B1, Class II Type B2
Class III

28

Describe HEPA filters

removed 99.99% airborne particles 0.3 um or greater. delicate construction. tested and certified for use in clinical labs

29

Describe Class 1 BSC

uncirculated air flows away from operator passes through HEPA & is discharged into atmosphere. operator protection. no product protection

30

Describe Class II BSC

most common. for working with microorganism in containment L 2,3,4 lab. other personal, product and environment protection.

31

how care Class II Type A (1and 2) and B (1 and 2) differentiated

by their construction, airflow velocity, airflow pattern and exhaust system

32

describe Class II type A1

cabinet air is either recirculated into lab or ducted out building by thimble connection after passing HEPA. not suitable for volatile chemicals or radionuclides. avg face velocity 0.38m/s. may have +ive pressure contaminated ducts

33

describe Class II type A2

cabinet air is either recirculated into lab or ducted out building by thimble connection after passing HEPA. suitable for low levels volatile toxic chemicals and radionuclides. avg face velocity of 0.5 mms. ducts and plenums under negative pressure

34

Describe Class II, Type B1

hard ducted. Exhaust passes through HEPA to atmosphere. suitable for low levels volatile chemical or radionuclides. contains -ive pressure plenums. min face velocity 0.5 m/s

35

Describe Class II, Type B2

hard ducted. exhausts 100% of cabinet air thru HEPA to atmosphere. contains -ive pressure plenums. face velocity 0.5m/s

36

Describe Class III

full air tight enclosure. HEPA filtered supply and exhaust air. cabinet is negative pressure work performed thru long sleeve gloves. protection for worker and product. good for group 4 organisms