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Flashcards in lecture 19 electrophoresis Deck (16):
1

define electrophoresis

movement of charged molecules in an external electric field

2

define anode

+ive electrode that attacts anions

3

define cathode

-ive electrode that attract cations

4

define electromotive force

drives analyte ion to electrode of oppose charge. needs to be higher than EOF

5

define electo endosmotic force (EOF)

bulk movement of water in opposite direction of anaylte ion

6

define isoelectric point

pH where net charge is zero

7

3 purposed of buffer

-provides ions for current
-set pH of system so molecules appropriately charged
-prevent pH change due to electrolysis of water

8

direction cations and anions migrate

cations to cathode (-ive electrode)

9

rlnshp between velocity (migration rate and voltage

higher the voltage faster the velocity

10

rlnshp between velocity (migration rate and IS

increased IS results in increase current faster

11

rlnshp between velocity (migration rate and molecular size

faster when size and is smaller, and spherical

12

rlnshp between velocity (migration rate and net charge

affected by ph. faster when more -ve charges moves closer to anode (+ive electrode

13

rlnshp between velocity (migration rate and [gel]

slower when [gel] is higher

14

direction of DNA travel

DNA either uncharged or -ive charge. move to anode (+ electrode)

15

why DNA has constant charge to mass ratio at pH 8

increase in -ive charged environement.move to anode (+ electrode)

16

why serum protein electrophoresis performed at pH 8.6

so proteins will have net -ive charge and migrate to anode (+ive electrode)