Lecture 4 Part 1: Viruses of Diarrhea

Question 1

Do rotaviruses have an envelope?

Answer 1

no envelope

Question 2

What is the structure of the rotavirus capsid?

Answer 2

Icosahedral, double layered capsid

Question 3

What type of genome do rotaviruses have?

Answer 3

dsRNA

Question 4

To which virus family do rotaviruses belong?

Answer 4

Reoviridae family

Question 5

How many genomic segments do rotaviruses have?

Answer 5

11

Question 6

What genetic process is possible due to rotavirus segmentation?

Answer 6

ressortment

Question 7

How are new infectious forms of rotavirus produced in the body?

Answer 7

Proteolytic cleavage in GI tract

Question 8

What is the name of the infectious form of rotavirus after cleavage?

Answer 8

ISVP – Intermediate/Infectious Subviral Particle

Question 9

How is rotavirus transmitted?

Answer 9

by fecal oral route

Question 10

What type of infection does rotavirus cause?

Answer 10

Lytic Infection

Question 11

What illness is caused by rotavirus?

Answer 11

Gastroenteritis

Question 12

In which population is rotavirus the most common cause of serious diarrhea?

Answer 12

young children

Question 13

slide 7

Question 14

What does the outer capsid of rotavirus resemble?

Answer 14

An envelope (though the virus is non-enveloped)

Question 15

What is the function of VP6 in rotavirus?

Answer 15

capsid protein

Question 16

What is the function of VP7 in rotavirus?

Answer 16

capsid protein

Question 17

What is the function of VP4 in rotavirus?

Answer 17

attachment protein

Question 18

What is the role of VP1 in rotavirus?

Answer 18

polymerase

Question 19

What is the role of VP2 in rotavirus?

Answer 19

transcriptase

Question 20

What is the role of VP3 in rotavirus?

Answer 20

mRNA capping protein

Question 21

What is NSP4, and what are its functions?

Answer 21

NSP4 is a viral protein expressed in the ER; it facilitates ER binding and also acts as an endotoxin

Question 22

Which group of rotaviruses is primarily responsible for clinical disease?

Answer 22

Group A

Question 23

What type of viral activity causes damage to the intestinal villi?

Answer 23

Lytic Production

Question 24

What is the consequence of damage to intestinal villi by rotavirus?

Answer 24

decreased water absorption

Question 25

What does decreased water absorption lead to in rotavirus infection?

Answer 25

diarrhea and dehydration

Question 26

What immune response occurs in the lamina propria during rotavirus infection?

Answer 26

Macrophage infiltration due to inflammation

Question 27

What are the common symptoms of rotavirus infection?

Answer 27

Vomiting, diarrhea, fever, and dehydration

Question 28

Caliciviruses

Question 29

Do caliciviruses have an envelope?

Answer 29

no envelope

Question 30

What is the shape of the calicivirus capsid?

Answer 30

Icosahedral

Question 31

What type of genome do caliciviruses have?

Answer 31

ss(+)RNA viruses

Question 32

Is the calicivirus genome segmented or linear?

Answer 32

linear

Question 33

Where do caliciviruses replicate in the host cell?

Answer 33

cytoplasm

Question 34

What type of infection do caliciviruses cause?

Answer 34

Lytic infection

Question 35

Which two genera of caliciviruses cause diarrhea?

Answer 35

Noroviruses and Sapoviruses

Question 36

Are caliciviruses resistant to harsh environmental conditions?

Answer 36

Yes, they are resistant to acid and heat exposure