Lecture 6: Olfaction and Gustation Flashcards Preview

Neuro I Exam 3 - Physiology > Lecture 6: Olfaction and Gustation > Flashcards

Flashcards in Lecture 6: Olfaction and Gustation Deck (35)
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1

Where are the odorant receptors (OR) located and how many do humans express?

- On the cilia of olfactory epithelium

- Humans express 350 different odorant receptors

2

What type of receptor is the odorant receptor and what is the cascade upon binding of an odorant?

GOlf stimulates adenylyl cyclase, which increases cAMP, which open cyclic nucleotide gated channnels (CNGC)

- Leads to the influx of cations (Na+ and Ca2+) and depolarization of the membrane

3

What occurs that allows us to "get used to the smell" of an odorant after stimulation persists for some time?

- Sensitivity of the CNGC to cAMP decreases, reducing cation influx

- A smaller generator potential results, reducing the probability of an AP in the axon

4

What are the odorant receptors like on one olfactory neuron and where do they project to?

- One olfactory neuron expresses the same odorant receptor on ALL of its cilia

- The projections of similar odorant receptors are collected into one glomerulus

5

How are different odorant receptors distributed in the olfactory epithelium?

- Localized to different part of the olfactory epithelium

- But are not equally between these areas

- Each zone (area) has a different receptor type that predominates

6

What is the advantage to the distributed localization of odorant receptors within the olfactory epithelium?

- If a part of the olfactory epithelium is damaged or blocked, you will likely be able to still detect an odorant.

- A receptor for a certain odorant is distributed in certain areas throughout the epithelium

7

Each odorant acts on how many recpetors and is the significance of the concentration of an odorant?

- Each odorant activates different combination of odorant receptors to produce its characteristic odor

- Odorant concentration changes the perceived smell

8

Through what mechanism is a higher odorant concentration able to change the perceived smell?

- At higher concentrations, the odorant starts to bind to receptors with a lower affinity for the odorant in addition to the receptors with a higher affinity

- At low concentrations, indole smells floral, but at high concentrations, it smells putrid!

9

What are the smaller, trace-amine associated receptors (TAARs) for odorants?

- Produce physiologic/endocrine responses to pheromones 

- Many of the chemicals that activate TAARs are found in the greatest concentration in urine from the male of the species being studied

10

The olfactory bulb consists of how many layers and contains what cells?

- Consists of 5 layers

- Mitral cells

- Periglomerular cells

- Tufted cells

- Granule cells

11

Within the olfactory bulb are the glomeruli where the axon terminals from olfactory neurons synapse and release what NT to what cells?

- Release EAA (glutamate) on the dendritic trees of the post-synaptic neurons:

Mitral cells

Tufted cells

Periglomerular cells

12

Which 2 cells of the olfactory bulb have axons that will go to the olfactory cortex?

1) Mitral cells

2) Tufted cells

13

Which 2 cells of the olfactory bulb are the local interneurons that release GABA?

1) Granular cells

2) Periglomerular cells

14

All the axons from the olfactory neurons expressing the same odorant receptor converge on how many glomeruli, located where?

- Converge on two glomeruli (one medial, one lateral)

- Located in the ipsilateral olfactory bulb

15

What is the function of periglomerular cells releasing GABA at their synaptic contacts with other glomeruli?

- Inhibits the activity from those glomeruli of related odorants

- This means that the odorant producing the strongest stimulation will suppress the input from other glomeruli that weren't as strongly activated

- Improves the signal specificty in the brain

16

What is the function of granular cells releasing GABA at their synaptic contacts with mitral and tufted cells?

They also work to increase the specificity of the message going to the olfactory cortex

17

Where is the first possible synapse within the olfactory cortex; where is info relayed from here?

- Anterior olfactory nucleus

- These synapses relay the info to the contralateral side

- Axons also continue on to the ipsilateral side

18

The olfactory cortex is composed of 5 areas of interest?

1) Anterior olfactory nucleus

2) Piriform cortex

3) Anterior cortical amygdaloid nucleus

4) Periamygdaloid cortex

5) Lateral entorhinal cortex

19

What is the importance of the olfactory output from the entorhinal cortex and where does it project?

- Projects to the hippocampus, which is important in memory formation

- Olfactory input facilitates both memory formation AND recall

 

20

What is the importance of the olfactory output from the piriform cortex and where does it project?

- Projects to the lateral hypothalamus

- Important in the control of appetite

- Olfactory inputs influence hunger and appetite

21

The piriform cortex also relays olfactory input to the thalamus and then medial orbitofrontal cortex which plays a role in?

- Integration of taste, sight, and smell

Appreciation of the FLAVOR of food

22

Why does the olfactory cortex also send input BACK TO the olfactory bulb/olfactory epithelium?

Modifies receptor responses to odorants

23

What is the importance of the anterior cortical amygdalaoid nucleus processing olfactory input?

Emotional learning; olfactory fear conditioning

24

Explain the mechanism for the transduction of Sour tastes (i.e., ions involved and NT released).

- Evoked by H+ ions entering cell

- Once inside causes a proton sensitive K+ channel to shut down

- Accumulation of positive charges = depolarization

Serotonin (5-HT) release

 

25

Explain the mechanism for the transduction of Salty tastes (i.e., ions involved and NT released).

Epithelial Na+ channel (ENaC)

- Increases the [Na+] in saliva and on the tongue

- Na+ and/or Ca2+ diffuses down gradient into cell and depolarizes membrane

Serotonin (5-HT) release

26

Explain the mechanism for the transduction of sweet, bitter, and umami (savory) tastes through the Type II cell (i.e., receptors, ions, and NT).

- Tastant binds to receptor (GPCR) and Phospholipase Cbeta2 is activated

- DAG and IP3 released; IP3 causes release of Ca2+ from the ER

- Ca2+ activates a TRPM5 receptor, allowing Na+ into the cell

Depolarization produced by released Ca2+ and Na+ open a gap junction subunit and ATP leaves the cell throough Panx1 channels

27

Where are the secondary sensory neuron cell bodies located in the ascending taste pathway; where do neurons ascend to and finally terminate?

- Nucleus tractus solitarius

- Ascend to synapse on tertiary sensory neuron cell bodies in: VPM of the Thalamus

- Finally ascend through posterior limb of the internal capsule and terminate in the postcentral gyrusfrontal operculum, and insular cortex

28

What is the pathway for integrating visual, somatosensory, olfaction and gustatory stimuli?

VPM of thalamus ---> Gustatory Cortex ---> Orbitofrontal Cortex

29

Perception of flavor requires what 3 inputs?

1. Gustatory input from the gustatory cortex

2. Olfactory input from the olfactory cortex (especially piriform cortex)

3. Somatosensory information from the mouth

*Remember the piriform cortex takes olfactory inputs and projects to the thalamus and then medial orbitofrontal cortex to provide info used to identify the flavor of foods!

30

Neurons from all three areas of the cortex (gustatory, olfactory, somatosensory) project where to give us the sensation of flavor and appreciation of food?

Lateral posterior orbitofrontal cortex