Lecture 7

Question 1

What is the MHC?

Answer 1

Set of closely linked genetic loci that are overwhelmingly important in determining fate of gengrafted tissue

Question 2

Is MHC polymorphic?

Answer 2

Highly

Question 3

Main function of MHC?

Answer 3

Presenting peptide to T lymphocytes

Question 4

What is HLA?

Answer 4

Tightly linked gene cluster of cell surface glycoproteins
Regulate immune cell interactions
Evoke allograft rejections

Question 5

What does HLA stand for?

Answer 5

Human leukocyte antigen

Question 6

What are the class I loci?

Answer 6

HLA
HLAB
HLAC

Question 7

Class II Loci?

Answer 7

HLADP
HLADQ
HLADR

Question 8

Where are class I loci found?

Answer 8

Surface of nearly all nucleated cells presenting antigen to CD8+ T cells

Question 9

Where are class II loci found?

Answer 9

DCs
B lymphocytes
Macs
Present to CD4+

Question 10

What presents to CD?

Answer 10

Class I

Question 11

What presents to CD4?

Answer 11

Class II

Question 12

What are class III loci?

Answer 12

Reside in MHC region but no involved in antigen presentation

Question 13

Where is the HHLA complex found?

Answer 13

Short arm chromosome VI

Question 14

What do class I molecules associate with?

Answer 14

Beta2 microglobin in cell membranes?

Question 15

Where in MHC is B2microglobin coded for?

Answer 15

Not in MHC, on different chromosome

Question 16

What is B2microglobin?

Answer 16

Lighter chain on cell surface that associates with class I molecule

Question 17

Structure of Class I?

Answer 17

Alpha chain
Class I polypeptide
B2microglobin

Question 18

What type of bond does MHC form with antigen?

Answer 18

Non covalent

Question 19

What varies amongst MHC molecules?

Answer 19

The alpha chain - varies in aa sequence

B2 microglobin is same in them all

Question 20

What portion of MHC crosses cell membrane?

Answer 20

C terminus

Question 21

What is class II molecule made of?

Answer 21

Alpha chain - 34k daltons

Beta chain - 29k daltons

Question 22

What does MHC I bind?

Answer 22

Short peptides 8 - 10 aa in length

Question 23

What does MHC class II bind?

Answer 23

13 - 17 aa peptides

Question 24

Where are MHCII alpha/beta and I alpha chains coded for?

Answer 24

Different genens on MHC locus

Same chromosome

Question 25

How are MHC genes expressed?

Answer 25

``` Co dominant manner Each person has 3 maternal and 3 paternal class 1 types Also has 3 maternal and 3 paternal II types ```

Question 26

What does T cell response depend on?

Answer 26

Recognition of presented antigen | Recognition of the MHC it is presented in

Question 27

What is CD8+ specific for?

Answer 27

Antigen in MHCI

Question 28

What is CD4+ specific for?

Answer 28

Antigen in MHCII

Question 29

What is MHC restriction?

Answer 29

Refers to T cells being specific for both antigen AND mhc that presents it

Question 30

What are some APCs?

Answer 30

Macs DCs B lymphocytes

Question 31

What does cytotoxic T cell do when recognizes MHCI and peptide?

Answer 31

Kills cell

Question 32

What is HLA?

Answer 32

Major histocompatability complex for humans

Question 33

Would MHC be the same in identical twins?

Answer 33

Likely not, highly polymorphic

Question 34

How many polypeptide chains in class I?

Answer 34

1

Question 35

How many polypeptide chains in class II?

Answer 35

2

Question 36

What domain is antigen binding to?

Answer 36

Alpha 1 & 2 helixes

Question 37

What are anchor residues?

Answer 37

Highly conserved areas on antigen allowing it to bind to certain MHCI

Question 38

Can MHC bind one or many peptides?

Answer 38

Many

Question 39

How is MHC inherited?

Answer 39

Strictly mendalian

Question 40

Men or women better organ donors?

Answer 40

Women, men have some protein women don?t

Question 41

How many MHC on a cell surface?

Answer 41

5k

Question 42

What can MHC convey to a person?

Answer 42

Genetic survival advantage

Question 43

What are T cells recognizing I MHC?

Answer 43

Non polymorphic region