Lecture 74 Flashcards
Most fungal membranes don’t contain cholesterol. Instead, they contain ______.
Ergosterol
Molds produce mycelia which consist of ______ and spores (aka conidia).
Hyphae
Mycelia can be septal, meaning they are _____nucleate, true hyphal syncitia, or nonseptal like in yeast which form pseudomycelia that are aseptal and are not ______nucleate.
Multinucleate
Multinucleate
Ascomycetes are fungi that make sexual spores that go into a ____ (ascus). These include Penicillin, Neurospora, Aspergillis, and Saccharomyces.
Sac
Basidomycetes are fungi that form ______. These include Amanitis, Porcini, and Cryptococcus.
Mushrooms
Zygomycetes are fungi that form ____-______ mycelia. These include Water molds, Phycomyces, and Mucor.
Non-septate mycelia
Deuteromycetes are fungi that have an unknown ____ cycle.
Sex
Fungi are routinely cultured on ________ agar which contains Peptone, Glucose, and agar. Most fungi grown on this agar will produce _____, but those that don’t have this cycle will produce ______.
Sabouraud’s agar
Molds
Yeast
Many fungi exhibit classical ________ dimorphism. This means that in Little Bear’s porridge, they grow in a yeast cycle, but in Mama Bear’s porridge they grow as mold. So yeast in our body, mold when cultured at room ____.
Temperature dimorphism
Temp
Diagnosis of fungal infection is by direct _____. Treating skin samples with 10%____ dissolves the mammalian tissue, but not the fungal cells.
Observation
10%KOH
Calcofluor is used to stain fungi that contain _____.
Chitin
_____ ink is used for staining CSF. Fungi that are encapsulated form a halo appearance.
India ink
Methane-amine-silver is used to the _______ protein on the fungal cell wall.
Mannan
ALL fungi stain with _____ _____, but they are not classified as gram pos or neg.
Crystal Violet
Some fungi that affect _____ and skin fluoresce using a long wavelength UV lamp (aka Wood’s lamp).
Hair
