lecture 9 Flashcards
what are the two steps that misfolded protein will trigger
stop any further protein synthesis
result in an expression of a particular set of proteins to help with the problem
what causes translation factor elF2a to bind to a small ribosomal subunit to inhibit function of translating protein
phosphorylation by PERK
what does pathway 2 in unfolded protein response do
ATF6 gets transported to Golgi and proteins will be transcribed to help with the overwhelming situation of misfolded proteins
where does the ER bud to create vesicles
RER where there are no ribosomes
whats the first step in vesicular transport
budding
what is the endoplasmic reticulum Golgi intermediate compartment
a compartment where all the vesicles can fuse and go to the Golgi together
whats the structure of Golgi complex
stack of flattened cisternae
what face of the golgi complex faces the ER
Cis Face
what side of the Golgi complex faces the opposite side of the stack
trans face
what modifies, stores, and secretes molecules that it receives from the ER
Golgi complex
what part of the Golgi is the protein sorting station
cis Golgi network
what part of the Golgi is the part that packages proteins into vesicles
trans Golgi network
whats the movement of protein through the golgi
cis to trans
what direction is the current model for vesicle transport ?
retrograde
what are the 2 functions of protein coating
causes membrane to curve and form budding vesicles
selects components to be carried by vesicle
what direction do COPII coated vesicles travel
forward
what direction do COPI coated protein travel
backward
where do clattering coated vesicles travel and what do they become
from the transgolgi network to becoming endosomes and lysosomes
what induces curvature in the membrane (vesicle formation)
COPII proteins and Sar1
what terminal signals where coated vesicles belong
C terminal
what kind of protein is Sar 1
G protein (requires GTP)
what do “sec” do
induce more curvature for vesicle formation
what are the 2 layers of transport vesicles
outer scaffold of proteins
inner layer of adaptor proteins
what is COPII created by? whats its lattice like?
sec13 and sec 31
no overlap of lattice
