Lecture Exam 3 Flashcards

(72 cards)

1
Q

Proteobacteria

A

Gram negative
Based on rRNA sequences
Derived from photosynthetic ancestors

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2
Q

Alpha proteobacteria

A
Azospirilum
Rhizobium
Nitrobacter
Rickeitsia 
Rickeitsia rickettsii 
Brucella 
Brucella abortus 
Brucella melitensis 
Wolbachia
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3
Q

Azospirilum

A

Soil organism
Nitrogen fixer
Symbiotic fixation; with roots of tropical grasses (corn)

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4
Q

Rhizobium

A

Soil organism
Nitrogen fixer
Symbiotic with legumes (beans, peas, clover, etc)

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5
Q

Nitrobacter

A

Soil organism
Not symbiotic
Chemoautotroph- using in organic compounds as its energy source
Take ammonia (NH3) or NO2 to NO3 (nitrification)

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6
Q

Rickeitsia

A

Rods/coccbicilli
Obligate intercellular parasite- cannot live outside of host cells
Requires an insect or tick as a vector (animal that carries it)

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7
Q

Rickeitsia rickettsii

A
Rocky Mountain spotted fever
Vectored by ticks
More common in the southeastern US
Symptoms: rash on palm and soles, fever, headache
Does respond to antibiotic therapy
No vaccine
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8
Q

Brucella

A

Coccobicillic- no flagella
Obligate parasite of mammals
Zoonose- animal reservoir of disease
Aerobic

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9
Q

Brucella abortus

A

Also known as Brucellosis
Causes cattle to spontaneous miscarry
Infects cattle, deer, elk, and bison

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10
Q

Brucella melitensis

A
Causes disease in humans
Causes undulant fever
Dairy, goats, sheep
Undulating fever- night sweats, aches and pains, fever goes up then down then up
Responds to antibiotics
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11
Q

Wolbachia

A

Obligate intercellular parasite
In insects
Very common
Affects the reproduction of the insect. Can cause more eggs, no change, or less eggs
Complex
*release of infected mosquitoes to control Zika and dengue fever

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12
Q

Beta proteobacteria

A

Bordella pertussis
Neisseria
N. Meningititis

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13
Q

Bordella pertussis

A

Coccobacilli
Capsule
Causes whooping cough (produces toxins that kill off the cillia in the trachea, mucous accumulate in lungs, and can go systemic)
Starts as cold symptoms, but becomes violent coughing with “whooping” inhalation
Can last weeks
Can kill infants, adults can be asymptotic
Can result in brain damaged kids
Does respond to antibiotics if caught early but not late
There is a vaccine for it

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14
Q

Neisseria

A

Diplococcus
Has fimbriae- used for attachment to mucous membranes
Neisseria gonorrhoeae- causes gonorrhea- STD “the clap”
-attacks mucosa of the urogenital track, oral cavity, anal, conjunctiva of the eye
- spread mainly by males
- diagnosed by pus discharge in males
- females it’s often asymptotic
-• can affect the cervix and cause reproductive issues
-• can cause pelvic inflammatory disease
- does respond to antibiotics but developing a resistance
- no developed immunity, can get it more than once

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15
Q

N. Meningitis

A

Diplococcus
Bacterial megingitis
40% of the population has it in their nose (asymptotic carriers)
Bacteremia to bacteria in blood stream, which produces a variety of toxins that result in a headache, that then produces a stiff neck, then a coma, then death. All of that can happen in hours
Usually hits infants under 2 years old
Does respond to antibiotics, but has to be caught quickly
Deaf and brain damage

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16
Q

Gamma proteobacteria

A

Legionella
Pseudomonas
P. Aeruginosa
Vibrio cholerae

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17
Q

Legionella

A

Sometimes called “legionare’s disease”
Symptoms are those of pneumonia (causes pneumonia)
Causes by contaminated water supplies aerosols
Difficult to culture

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18
Q

Pseudomonas

A
Gram negative rods
Have polar flagella
Common in various environments (water and soil)
They are diverse
Some cause plant diseases
One metabolic trick is they can digest unusual carbon sources
Antibiotic resistant
Not normally pathogenic
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19
Q

P. Aeruginosa

A

If you have a wound, especially burns, it will get in there
Abcesses- nor on pets
Urinary tract infections
You can get septicemia to toxins in blood to toxic shock syndrome

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20
Q

Vibrio cholerae

A

Curved rods- vibrio
It is a facultative anaerobe
Produces an exotoxin in your small intestine
Exotoxin stimulates secretion of water and electrocytes
DFH- 10-20 liters a day, can kill you even if you stay hydrated, physicians refer to this as “rice water stool”
Common inhabitants of brackish (salty water that is not salty enough to keep from drinking) water and fresh water
What killed most of the people on the Oregon trail

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21
Q

Enterics

A
Gram negative rods
Facultative anaerobes
Fermenters
They tend to have fibriae 
Attached to the intestines
E. Coli
Salmonella enterica 
Yersiha enterocolitica
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22
Q

E. Coli

A
Normally it’s ok 
Capable of causing mild diarrhea
We use E. Coli as an indicator of food and water contamination 
But there is a stain of e. Coli called 0157: H2. It will kill you
- produces an endotoxin
- “enterohemorrhagic”
- DFH
- most commonly kills elderly and kids
- in apple juice by contamination
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23
Q

Salmonella enterica

A

Difficult genus to classify
High genetic diversity
Use serovaris to diagnose them (identified with antibodies)
Dont normally find it in our intestines, but in birds and reptiles
Common source of food poisoning
Sometimes called “salmonellosis” and non-typhoidal- DFH
typhoidal-
-causes typhoid fever
- serovaris: S. Typhi
- is not carried by birds (not a zoonose)
- human to human- contact and food
- gets inside of white blood cells and spreads
- 2-3 week incubation period
- • fever headache, ulcerated intestines
- will kill people
- can have asymptotic carriers- likes to settle into the gallbladder
-“Typhoid Mary”- Boston in the early 1900s she was a domestic worker, everyone got typhoid fever.
- early study in Epidemiology

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24
Q

Y. Enterocolitica

A
In the intestines from domestic animals
Food contamination
Psycherotroph- will grow in the fridge
Fever, diarrhea, abdominal pain, etc
Can last 2 weeks
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25
Firmicutes
Low G+C ratio Rods and produce endospores Bacillus- facultative anaerobe, non- motile, endospores
26
B. Anthracis
``` Anthrax Soil and livestock Cutaneous form- “coal black lesions” Pulmonary/lung- 100% mortality rate Gastrointestinal ```
27
B. Cereus
Food poisoning Produce toxins- spores Diarrhea (most common in restaurant rice)
28
B. Thuringiensis
Produces a toxin- Bt toxin (protein) Kills caterpillars Biocontrol- harmless to humans- kills bugs Genetically modified organisms - corn and few other crops to produce the Bt toxin
29
Clostridium
``` Endospore forming rods Obligate anaerobe Soil organism Spores are tough and hard to kill- survive 120 degrees for 15 minutes C. Tetani C. Botulinum C. Perfringens C. Difficile ```
30
C. Tetani
``` Causes tetanus Associated with puncture wounds- anaerobic environments Grow to produce a neurotoxin (inhibits exocytosis of neurotransmitters of inhibitory neurons- muscles- “tetanospasmin” - muscle contracts, but can’t relax -• starts with jaw muscles (lock jaw) -•- proceeds from there -•- death from respiratory failure -•- incredibly painful Vaccine Antiserum ```
31
C. Botulinum
``` Latin for sausage Botulism Flacio paralysis Neurotoxin Canned foods and sausage (spores survive canning) 1-2 day incubation Lasts 1-10 days Toxin destroy by heat Nitrites (sausage) inhibit sporulation Rare Not in acid foods Honey- don’t give to infants (infant botulism) ```
32
C. Perfrigens
``` Causes gas gangrene Cytotoxin- kills cells Wound- anaerobic environment - bacterium grows - cytotoxin - kills tissue - bacterium grows Debridment/amputation ```
33
C. Difficile
``` Intestines- can be a component of the normal intestinal flora Antibiotics- wipe out normal flora Party time for C. Diff Toxin- DFH Nosocomial (hospital acquired) infection ```
34
Lactobacillus
Acidophile Aerotolerant anaerobe Normal flora- oral, intestinal, vagina Foods- yogurt, pickles, sauerkraut, kimchi
35
Staphylococcus
``` Grape like clusters Facultative anaerobes Tolerant high osmoticum (halo phones) On skin- up your nose S. Aureus ```
36
S. Aureus
``` Food poisoning- produces a toxin- diarrhea Ham/ custard Wounds- secondary infections Antibiotic resistance MRSA- Methicillin Resistant S. Aureus ```
37
Streptococcus
``` Chains Aerotolerant anaerobe Good: - lactic acid from fermentation (cheese) Bad: - strep throat - scarlet fever - pneumococcal pneumonia - necrotizing fasciitis Toxins- hemolytic, erthrogenic (scarlet fever), coagulate ```
38
Mycoplasma
``` No cell wall Pleomorphic- shape varies Sometimes filamenrous Small- 0.1-0.25 nanometers Colonies are small, but you can culture them- look like fried eggs ```
39
M. Pneumoniae
Mild pneumonia Sometimes called “walking pneumonia” Can cause arthritis in goats
40
Streptomyces
Soil organism | Source of streptomycin- good source of antibiotics
41
Mycobacteria
``` Acid-fast microbe Waxy cell walls Slow growing because of waxy walls M. Tuberculosis M. Leprae ```
42
M. Tuberculosis
Deadly lung infection Inhale bacteria - aerosols Connective tissue grows around lumps of bacteria (tubercles) -tubercles- “little potatoes” Antibiotic therapy But antibiotic resistance is now happening
43
M. Leprae
Causes leprosy/Hansen’s disease Affects the peripheral nervous system Obligate parasite- grows on armadillos
44
Corynebacterium
Pleomorphic Good chance it’s in your armpit Responsible for arm out odor C. Diphtheriae
45
C. Diphtheriae
Upper respiratory tract infection Diphtheria Droplet transmission Forms tough Greyson membrane in the back of the throat Vaccine Pre-vaccine it was the number one kid killer
46
Archaea
Figured out in the 70s Back to genetic evidence (RNA sequence) Many biochemical differences Both bacteria and eukaryia - when looking at lipid membranes they have an “ester” linkage - archaea have an ester linkage in their phospholipids - are in pretty much any habitants - many are extremophiles- live in 100 degree or more, some are halophiles, some are acidophilus, some only live in alkaline environments - do photosynthesis without using chlorophyll and instead use bacteriorhodosin(purple, not green) - many are mutualistic, especially in ruminants- helps with digestion of cellulose- h2+CO2, methanogens- 4H2+CO2-CH4+2H2O - also in the human gut- 1 in 10 are archaea, many are methanogens No pathogens that we know of
47
Kingdom fungi
Cell walls made of chitin Cells organized into tubes (hypha/hyphae/mycelium) Filaments Thalallus- organ or structure (mushroom) Hyphae can be either coencyte- nuclei loose in cytoplasm Hyphae can be septate- nuclei have separate capartments Nutrition: chemoheterotrophs- their absorbing chemicals as food, decay organisms, parasites, utilize many odd C source Reproduction: asexually- without sex, fragmentation- tear them apart, yeasts- budding, also produce spores Sexual reproduction: life cycle is backwards from us- spend most of their life cycle as a haploid(1N), diploid (2N)- short, brief Medical mycology- “study of fungi”- mycosis- fungal infection-usually slow growing, cutaneous- ringworm, athletes foot, keratinase- enzyme that breaks down keratin Subcutaneous mycosis- more rare, wounds, systemic mycosis- deep tissue- most common form in the lungs- histoplasmosis- most common if agriculture (hay baling)
48
Microsporidian
``` Obligate intercellular parasites Single celled Dont have mitrochondria No sex Conjunctivitis- pink eye Diarrhea ```
49
Yersinia pestis
``` Bubonic plague Black Plague Zoonose- animal reservoir Bubonic: “bubos”- swollen lymph nodes, black, painful, high fever, 75% death rate Pneumonic- lungs, inhalation, 100% fatal ```
50
Helicobacter pylori
``` Rod Microaerophile Flagella Peptic ulcer disease Barry marshal- biopsy always, Koch’s postulates, antibiotic therapy, Nobel prize ```
51
Cyanobacteria
``` Blue-green algae H2O + CO2- sugar+ O2 Photosynthesis- regular N fixation Ecologically huge Purple sulfur bacteria Anoxygenic photosynthesis Dont produce O2 H2S + CO2- sugar+ S2 ```
52
Chlamydia
``` STD- all too common Obligate intracellular parasite No vector Non- gonococcal urethritis Trachoma- blindness ```
53
Spirocheates
Axial filaments O2 requirement varies Aerobes- facultative anaerobe
54
Treponema pallidum
Syphilis- STD 3 phases: Primary - chancre sore, open sore at site of infection, painless, might not be obvious Secondary - oral sores, rash, very infectious, lantent- no obvious symptoms 2-4 years old Tertiary - gummas, skin lesion, weakened aorta, blond, neurosyphilis- CNS, painful- crazy Antibiotic resistance
55
Borrelia
``` Lyme disease Zoonose- deer to ticks to humans Bulls eye rash at bite site Flu like symptoms Fatigue- neurological problems Heart Meningitis Antibiotics are not always effective Difficult to diagnose ```
56
G+C ratio
DNA base pairing
57
Phylum zyhomycota
Sexual reproduction is via zygosporez Coenoctic- no cross walls Asexual stage is via sporanglospores No make it female rather mating types (+/-) 2 hypo is nuclei fuse, which gets us back to diploid, the perform meiosis to become haploid again
58
Rhizopus
Can infect wounds | Inhalation of spores can be bad- histoplasmosis
59
Ascomycota
Sac fungi Yeast Sexual stage- consists of 8 ascospores in a structure called Ascus Asexual conidiospores Diseases: athletes foot, ring worm, yeast infection, penicilium, ergot- plant disease of grasses (rye and wheat- fungus replaces the grain)
60
Basidiomycota
Club fungi- mushrooms and puffballs and stinkhorns Sexual stage- basidiospores Fuming body- mushroom Diseases: dandruff, meningitis caused by cryptococcus from epiduralanesthetc
61
Algae
Photosynthetic- live in water (fresh and marine) Medically important- dinoflagellates- unicellular floaters Blooms- turn H2O red Clams are filter feeders and concentrate neurotoxins You eat the clam- you get paralytic shell fish poisoning
62
Kingdom protista
Animals like: protozoan Unicellular- diverse Asexual- mitosis/fission, shizogony- multiple fission Sexual reproduction: crazy diverse- conjugation- haploid nuclei, swap micronuceli, then fuse the macro and micronuceli, diploid, meiosis, haploid Gametocytes- haploid sex cells, like sperm and egg, but look the same
63
Archaezoa
Used to be mastigophora Have flagella Typically spindle shaped- cigar shape Giardia
64
Giardia
Has flagella Undulating membrane DFH “Beaver fever”
65
Phylum platyhelmenthes
Trematodes (classes within phylum) Sometimes referred to as “flukes” because they are leaf shaped Paragonimus- lung fluke Blood fluke- shistomas/schistosomiasis
66
Blood fluke
``` Humans are definitive hossnails are the alternate host Problem in tropic (S. America) 250x10^6 infected Larvae can borrow through your skin Bladder and urinary system Intestines Systemic Can get into the brain ```
67
Class cestoda
Tapeworms Taenia- beef tapeworm Simple Dont have a gut, brain, eyes, etc Live in intestines- absorb nutrients Can get 6 meters long Proglottids fill up with eggs Eggs in feces Get eaten by cows Eggs hatch in cow Larvae migrate to muscle tissue and go format “Measly beef” cook steak rare and encrust wake up in you They can get into the diaphragm and eye muscles (rare) Pork tapeworm- common in Mexico and Latin America, intestines, brain, eyes
68
Phylum nematoda
``` Round worms Tube gut (mouth and anus) Many many are in soil Parasitic too Enterobius Ascaris Necktie Truchinella ```
69
Enterobius
``` Pin worms 1-2 cm Size of a pin Preferred habitat is large intestines Playing in the dirt and not washing your hands Mostly a problem with kids Scotch tape test ```
70
Ascaris
30 cm long Small intestines Eat eggs- larvae- lungs- throat- intestines-sex- eggs in feces Adults can escape- anus or Jose or puncture small intestines
71
Necator
``` Hookworm 1 cm or so One end hooked Common in SE US Suck blood in intestines Eggs escape in feces Larvae in soil Can borrow through skin ```
72
Trichinella/ trichinosis
Pigs/ people Adults in intestines- sex Larvae migrate to muscle tissue Encyst