Lecture: Lab Eval of Hemostasis

Question 1

Important in collagen synthesis

Answer 1

Vitamin C or ascorbate

Question 2

In Hess Test Positive Pressure Technique, BP cuff is inflated to _ for _ minutes

Answer 2

80mmHg
8 minutes

Question 3

Positive results of Hess Test Positive Pressure?

Answer 3

Presence of petechiae

Question 4

In Negative Pressure Technique, a modified _ instrument is used to inflate at _ for _ minute

Answer 4

da Silva Melle
200mmHg
1 minute

Question 5

Normal value of petechiae in Negative Pressure Method

Answer 5

Less than 4 petechiae

Question 6

Normal value of petechiae in Rumple-Leede

Answer 6

0-10

Question 7

Abnormal value of petechiae in Rumple -Leede

Answer 7

More than 20

Question 8

Equivocal value of petechiae in Rumple-Leede

Answer 8

10-20

Question 9

Composition of Reese and Eckers’

Answer 9

Citrate
Formaldehyde
Buffer
BCB

Question 10

Dilution factor of RBC in Toncantin’s

Answer 10

1:200

Question 11

Dilution factor of WBC in Toncantin’s

Answer 11

1:20

Question 12

Direct Method is also known as

Answer 12

Reese and Ecker’s
Toncantin’s

Question 13

Unopette System contains?

Answer 13

Ammonium oxalate

Question 14

Dilution factor utilized in Unopette System

Answer 14

1:100

Question 15

In Fonio’s Method, 10-40 RBC equates to _ platelet in its indirect counting estimation

Answer 15

1 platelet

Question 16

In Fonio’s Method, 100 RBC equates to _ platelet in its indirect counting estimation

Answer 16

3-10 platelets

Question 17

In Fonio’s Method, 200 RBC equates to _ platelet in its indirect counting estimation

Answer 17

5-20 platelets

Question 18

0-49,000/uL is reported as?

Answer 18

Marked Decrease

Question 19

50,000-99,000/uL is reported as?

Answer 19

Moderate Decrease

Question 20

100,000-149,000/uL is reported as?

Answer 20

Slight Decrease

Question 21

150,000-199,000/uL is reported as?

Answer 21

Low Normal

Question 22

200,000-400,000/uL is reported as?

Answer 22

Normal

Question 23

401,000-599,000/uL is reported as?

Answer 23

Slight Increase

Question 24

600,000-800,000/uL is reported as?

Answer 24

Moderate Increase

Question 25

Above 800,000/uL is reported as?

Answer 25

Marked Increase

Question 26

These disorders are what type of thrombocytopenia? Idiopathic thrombocytopenic purpura (ITP) Post-transfusion Heparin-induced

Answer 26

Primary Thrombocytopenia

Question 27

These disorders are what type of thrombocytopenia? Systemic lupus erythematosus (SLE) Viral infection Lymphoproliferative disorders

Answer 27

Secondary Thrombocytopenia

Question 28

An abnormal increase in the number of circulating platelets as a result of physiologic or pathologic processes

Answer 28

Thrombocytosis

Question 29

These disorders are what type of thrombocytosis? Myelodysplastic Syndromes (MDS) such polycythemia vera and essential thrombocythemia

Answer 29

Primary Thrombocytosis

Question 30

These disorders are what type of thrombocytosis? After hemorrhage IDA Malignancy Epinephrine administration

Answer 30

Secondary Thrombocytosis

Question 31

In vivo platelet function and number test

Answer 31

Bleeding Time

Question 32

Subjective pressure More practical and convenient Finger pricking and earlobes

Answer 32

Duke's Method

Question 33

Better method based on its principle Puncture forearm with blood pressure cuff

Answer 33

Ivy’s Method

Question 34

Cuff is inflated to _ in Ivy's Method

Answer 34

40mmHg

Question 35

Almost the same with Duke’s but the difference is immersion of puncture finger in sterile physiologic saline solution (NSS) warmed at 37C water bath

Answer 35

Adelson-Crosby Method

Question 36

Same as Adelson-Crosby Method

Answer 36

Cody-Lalitch

Question 37

Same principle as Adelson-Crosby Method but it only uses earlobe as the site of puncture

Answer 37

MacFarlane for Bleeding Time

Question 38

Assesses the effect of a standard dose of aspirin on the Duke’s Bleeding Time

Answer 38

Aspirin Tolerance Test

Question 39

In vitro determination of platelet adhesiveness

Answer 39

Platelet Retention Test (Glassbead Retention Test)

Question 40

These disorders are abnormally decreased or abnormally increased in platelet retention test? Bernard-Soulier Glanzmann Thrombastenia vWD Chediak-Higashi Myeloproliferative Disorder Uremia Aspirin Administration

Answer 40

Decreased

Question 41

These disorders are abnormally decreased or abnormally increased in platelet retention test? Thrombotic Disorders Hyperlipidemia Carcinoma Oral Contraceptives Pregnancy

Answer 41

Increased

Question 42

Principle of Platelet Aggregation Evaluation

Answer 42

Turbidimetry / Transmittance of Light

Question 43

What disorder is it where upon adding ristocetin, it fails to aggregate?

Answer 43

Bernard-Soulier Syndrome

Question 44

What disorder is it when adding ECA, it abnormally reacts?

Answer 44

Glanzmann Thrombasthenia

Question 45

Too low platelet, high transmittance

Answer 45

PPP (protein-poor plasma)

Question 46

Too high platelet, low transmittance

Answer 46

PRP (protein-rich plasma)

Question 47

Anticoagulant used in testing platelet aggregation

Answer 47

Citrated blue top

Question 48

Last added aggregating agent

Answer 48

Epinephrine

Question 49

First to be added and has broad curve

Answer 49

Ristocetin

Question 50

Aggregating agents that cause biphasic curve

Answer 50

Epineprhine Thrombin ADP

Question 51

Weak aggregating agent; 30% T

Answer 51

Serotonin

Question 52

It reflects the number and quality of platelets, fibrinogen concentration, the fibrinolytic system and the packed cell volume

Answer 52

Clot Retraction

Question 53

Using castor oil for constipation; (+) dimpling phenomenon for 15-45 minutes; Mickey mouse appearance

Answer 53

Hirschboeck Method

Question 54

Blood is allowed to clot in a test tube containing a glass rod and retraction is observed after the incubation period. The clot attached to the rod is then removed and the serum extracted from the clot is measured.

Answer 54

MacFarlane for Clot Retraction

Question 55

Normal Value for MacFarlane for Clot Retraction

Answer 55

44-67% for both methods

Question 56

Blood in a test tube used for clotting time (one of the 3 tubes in the Lee and White Method is used) is saved and left at room temperature in order to note retraction, red cell fall out and clot lyses

Answer 56

Single Tube Method

Question 57

Measures clotting time then compare the result, promote clotting If PRP is near control, normal If PPP is near control, abnormal

Answer 57

PF3 Availability

Question 58

Platelet are activated, change shape, release granules; Presence means on going platelet activation; Method is radioimmunoassay or RIA

Answer 58

PF4 and BTG