Lesson 6 (Biosafety and Biosecurity)

Question 1

What do you call the pathogens that are commonly acquired

Answer 1

Laboratory Acquired Infections (LAI)

Question 2

Protecting people from dangerous pathogens

Answer 2

Biosafety

Question 3

Protecting pathogens from dangerous people

Answer 3

Biosecurity

Question 4

When and where did the history of laboratory biosafety took place

Answer 4

North America and West Europe (1943)

Question 5

The first person who created the Biological Development/ Biological Weapons Program

Answer 5

Franklin Roosevelt

Question 6

When did he create the Biological Weapon Program

Answer 6

during cold war

Question 7

(blank) focused only on the biological usage or microorganism that are against the (blank)

Answer 7

Biological Program; Soviet Union

Question 8

The first scientific director in 1943 of Camp Detrick

Answer 8

Ira L. Baldwin

Question 9

The center of the US Biological Program and also the designated installation for Biological Research and Development

Answer 9

Camp Detrick

Question 10

new name for Camp Detrick

Answer 10

Fort Detrick

Question 11

Designed multiplications for Biosafety at Camp Detrick

Answer 11

Newell Johnson

Question 12

The meetings of Johnson eventually lead to (blank)

Answer 12

The American Biological Safety Association (ABSA)

Question 13

He terminated the Biological Program that created by Roosevelt + (what year)

Answer 13

Richard Nixon in 1969

Question 14

First person who invented the Mechanical Pipettor

Answer 14

Arnold Wedum

Question 15

Another invention of Arnold Wedum

Answer 15

Ventilation Cabinet to prevent infection against MTB or Macrobacterium Tuberculosis

Question 16

Defined as any biological or chemical substance that is dangerous to
human, animals or the environment

Answer 16

Biohazard

Question 17

Examples of biohazard

Answer 17

body fluids, human tissue and blood, and recombinant DNA

Question 18

Used universally to report Harmful substances that are particularly
dangerous for living beings

Answer 18

Biohazard symbol

Question 19

Who created the Biohazard symbol (year)

Answer 19

Charles Baldwin (1966)

Question 20

Agents that are not associated with disease in healthy adult
humans. The low individual and community risk.

Answer 20

RG 1

Question 21

Agents that are associated with human disease which is
rarely serious and for which preventive or therapeutic inventions are
often available. Moderate individual and low community risk.

Answer 21

RG 2

Question 22

Agents that are associated with serious or lethal human
disease for which preventive or therapeutic or interventions may be
available. High individual risk but low community risk.

Answer 22

RG 3

Question 23

Agents that are likely to cause serios or lethal human disease
for which preventive or therapeutic inventions are not usually
available. High individual risk and high transmissible to an
individual.

Answer 23

RG 4

Question 24

Suitable for work involving variable microorganism that are defined
and well characterized strains known not to cause disease in human; appropriate among undergraduate and secondary educational
training and teaching laboratories that require basic lab safety
practices; no containment needed

Answer 24

Biosafety lvl 1

Question 25

Containment for Biosafety lvl 1

Answer 25

no containment needed

Question 26

Ex. for biosafety lvl 1

Answer 26

Bacillus subtilis, Naegleria gruberi, Infectious canine hepatitis
virus

Question 26

Risk Group 1 Organism

Answer 26

E coli K-21
Transgenic Plants
Plasmids
Fungi
Molds
Yeast

Question 27

For lab that deals with indigenous MODERATE RISK agents; lab consequently observing good microbial techniques; when handling human blood, body fluid, tissues where there is
uncertain presence of infections agents; handwashing sinks and waste decontamination facility

Answer 27

Biosafety lvl 2

Question 28

Containment for Biosafety lvl 2

Answer 28

biosafety cabinets

Question 29

Ex. for biosafety lvl 2

Answer 29

Hepa B, HIV Salmonella, Toxoplasma

Question 30

Risk Group 2 Organism

Answer 30

Human or Primate Cells
Herpes simplex Virus
Replication Incompetent Attended Human
Immunodeficiency Virus
Patient specimens

Question 31

Primary and secondary barriers in the protections of the personnel
from infectious aerosol exposure; indigenous or toxic agents with potential for respiratory transmission that may cause serious and lethal infections

Answer 31

Biosafety lvl 3

Question 32

Ex. for biosafety lvl 3

Answer 32

MTB, St. louis Encephalitis Virus

Question 33

Risk Group 3 Organisms

Answer 33

Human Immunodeficiency Virus
Mycobacterium Tuberculosis
Coxiella Bumetii

Question 34

Dangerous and exotic agents that pose high individual risk of
life-threatening disease that may be transmitted via aerosol
No vaccine or treatment
Separate building or completely isolated zone
Builds on BSL-3 / ABSL-3 practices

Answer 34

Biosafety lvl 4

Question 35

Containment for Biosafety lvl 4

Answer 35

BSL-3 + Class III Biosafety Cabinet

Question 36

Risk Group 4 Organisms

Answer 36

Lassa Fever Virus
Ebola Hemorrhagic Fever Virus
Marburg Virus
Herpes B Virus

Question 37

meaning of HEPA filtered air

Answer 37

High Efficiency Particulate Air

Question 38

Provides protection foe the user and surrounding
environment, but no protection for the sample being
manipulated

Answer 38

Biosafety Cabinets Class 1

Question 39

provides protections for the user, environment
and sample, and divides into four types: A1, A2, B3 and
B2. The main difference are their minimum inflow
velocities and exhaust system

Answer 39

Biosafety Cabinet Class 2

Question 40

Also known as gloves boxes
Provides maximum protections; he enclosure is gas
light and all materials enter leave through a dunk tank
or double door autoclave

Answer 40

Biosafety Cabinet Class 3