Limiting Factors Affecting Enzymes: non-competitiveInhibitors

Question 1

Non-competitive inhibitors

Answer 1

bind to the enzyme at an alternative site, which alters the shape of the active site and therefore prevents the substrate from binding to it

Question 2

Metabolic reactions can be controlled by using the end-product of a particular sequence ………….

Answer 2

of metabolic reactions as a non-competitive, reversible inhibitor:

Question 3

Metabolic reactions can be controlled by using the end-product of a particular sequence of metabolic reactions as a non-competitive, reversible inhibitor how

Answer 3

As the enzyme converts substrate to product, the process is itself slowed down as the end-product of the reaction chain binds to an alternative site on the original enzyme,

changing the shape of the active site and preventing the formation of further enzyme-substrate complexes

Question 4

end-product inhibition

As the enzyme converts substrate to product, the process is itself slowed down as the end-product of the reaction chain binds to an alternative site on the original enzyme, changing the shape of the active site and preventing the formation of further enzyme-substrate complexes

what happens after this

Answer 4

The end-product can then detach from the enzyme and be used elsewhere, allowing the active site to reform and the enzyme to return to an active state

This means that as product levels fall, the enzyme begins catalysing the reaction once again, in a continuous feedback loop

This process is known as end-product inhibition

Question 5

Increasing the concentration of an inhibitor, therefore, ………… the rate of reaction and eventually, if inhibitor concentration continues to be increased, the reaction will ……… ……….

Answer 5

reduces

stop completely

Question 6

For non-competitive inhibitors, increasing the substrate concentration can …………..

Answer 6

cannot increase the rate of reaction once more, as the shape of the active site of the enzyme remains changed and enzyme-substrate complexes are still unable to form

Question 7

Non-competitive inhibitors lower the initial rate of reaction and ………….

Answer 7

and the maximal rate of reaction (a lower amount of product is produced than would normally be produced).