Required practical 1 enzyme controlled reaction

Question 1

step 2 add …. of the solution of milk powder to ….

Answer 1

10cm cubed 3%

each of the three test tubes

Question 2

step 1 using a marker ………..

Answer 2

pen write an ‘X’ on the glass halfway down one side of each of three teste tubes

Question 3

step 3 add 2cm cubed of ……… to 2 cm cubed of ……. in another set of three …….

Answer 3

trypsin solution

ph 7 buffer

test tubes

Question 4

step 4 stand the three test tubes containing the solution of ………. and the three test tubes containing ….. in a water bath at ……

Answer 4

milk powder

trypsin and buffer

20 degrees

Question 5

step 5 leave all 6 tubes for …..

Answer 5

10 mins

Question 6

step 6 Add the trypsin and buffer solution from one test tube to ……….

Answer 6

the solution of milk powder in another test tube

Question 7

step 7 put a ……….. in the test tube and invert about ….. times to mix thoroughly

Answer 7

bung or cork

5 times

Question 8

step 8 put the test tube back into the

Answer 8

water bath

Question 9

step 9 repeat steps 6 and 7 using the other …..

Answer 9

test tubes

Question 10

step 10 Time how long it takes milk to go ……….. . Do this by measuring the time taken to ………..

Answer 10

clear

to first see the X through the solution

Question 11

step 11 record the

Answer 11

time for the three experiments

Question 12

step 12 using same method find how long …………. at ……….

Answer 12

Trypsin to digest the protein in the solution of milk powder at 30,40,50,60 degrees

Question 13

step 13 and 14 record the data in a suitable table and process the data and draw a graph what is the formula for rate of reaction

Answer 13

1 over time

1/time

Question 14

How would you set up acontrol and why do we use it

Answer 14

Add the milk powder and buffer but not enzyme In short, controls are essential for the unbiased, objective observation and measurement of the dependent variable in response to the experimental setup.