Live Cell Imaging Answers

Question 1

what is live-cell imaging (2)

Answer 1

1) imaging technique used to observe cells in their mimicked natural environment in real-time
2) study cell functions using microscopy

Question 2

What is live cell imaging used for (6)

Answer 2

1) cell behaviour
2) Cancer research
3) Cell signalling and interaction
4) Drug research
5) Neuronal interactions
6) tissue engineering

Question 3

seven steps of live cell imaging

Answer 3

1) plate cells and incubate for growth
2) treat cells with desired compound
3) Stain cells with fluorophores
4) environmental controls to mimmick the cells natural environment
5) obtain the image using the proper cell imaging technique based of the environmental conditions and what you intend to analyze
6) Analyze the image using software of choice
7) name the type of live cell imaging technique and explain in 1-2 sentences how it works

Question 4

Bright-field microscopy

Answer 4

the specimen is illuminated with white light passing through it, images are formed when observing differences in light absorption withing the sppecimen (primarily 2D)

Question 5

TIRFM

Answer 5

involves pointing a laser at specific angles of the specimen. Only fluorophores between 10 and 200 nanometers are excited, thus generating a clear image of the surface of the specimen (2D)