Flashcards in liver histology Deck (24):
1. Describe the basic pattern of blood flow through the liver
Liver receives the majority (70-75%) of its blood supply from the hepatic portal vein and about 25-30% from the hepatic artery which branches from the celiac trunk. The hepatic veins drain the liver, emptying into the inferior vena cava. These vessels and the bile duct enter and leave the liver in a hilar region called the porta hepatis.
Functions of liver
It synthesizes most of the blood proteins, glycoproteins, and lipoproteins, stores glucose from the gut temporarily in the form of glycogen, metabolizes many lipid-soluble molecules and toxins, and is involved in urea formation. Also production of bile
List the three lobular descriptions of the basic microstructural organization of the liver.
Classical lobule, portal lobule and acinar lobule. The lobules are the smallest individual functional units within the liver
Structure of classic lobule
Hexagonal arrangement of anastomosing hepatocytes arranged radially around a central vein. At each of six vertices is a bile duct, a lymphatic space (space of mall) and interlobular vessels carrying deoxygenated blood from hepatic portal vein and oxygenated blood from hepatic artery. Distributing or perilobular branches from interlobular vessels extend peripherally around edges of lobules distributing blood into sinusoids btw plates of hepatocytes
what is the portal triad
The interlobular vessels (venules and arterioles) and bile duct surrounded by loose CT stroma
Describe blood flow in classic lobule
Blood from the perilobular branches is distributed into sinusoids btw plates of hepatocytes. Blood percolates through the narrow sinusoids between the plates, en route to the central vein.
How thick are the hepatocyte plates in classic lobule
one hepatocyte thick
Bile flow in classic lobule
A network of tiny bile canaliculi run within the plates of hepatocytes, sealed from exposure to the
blood by zonulae occludens between hepatocytes. The network of canaliculi is peripherally connected to the bile ducts running along each vertex of the lobule A network of tiny bile canaliculi run within the plates of hepatocytes, sealed from exposure to the
blood by zonulae occludens between hepatocytes. The network of canaliculi is peripherally connected to the bile ducts running along each vertex of the lobule
Describe a portal lobule structure
Portal lobule is defined as the roughly triangular shape drawn between three central veins. This area defines a zone of tissue around a bile duct into which a group of bile canaliculi feed, hence defines a basic “bile secretory” functional unit.
Describe an acinar lobule
The “short axis” lies between two portal triads (one edge of the “border” of a classic lobule).The “long axis” lies between two central veins. The acinar lobule defines liver tissue in terms of blood delivery and is divided into 3 different zones.
4. Describe the functions of the hepatocyte.
1. Store glucose as glycogen after meal and converts glycogen to glucose while fasting, 2. produce blood proteins such as albumin, fibrinogen and prothrombin, lipoproteins VLDL, LDL, IDL and HDL 3. take up lipid soluble toxins from intestine, bilirubin from spleen and detoxifies them. 4. eliminates excess cholesterol in bile
5. Describe the relationship of the hepatocyte to the blood and bile from a structural viewpoint
Hepatocytes are arranged in anatomosing sheets/plates one cell thick, so two sides of the hepatocytes are exposed to blood sinusoids. Bile canaliculi run within the plates of hepatocytes
Components of hepatocyte involved in detox
SER (hypertrophies upon exposure to toxins or alcohol), enzymes in SER, enzymes in peroxisomes
6. Be able to define the arrangement of the liver sinusoidal endothelial cells and Kupffer cells within the liver.
Between each hepatocyte plate is the sinusoid which contains a fenestrated layer of endothelial cells which allows free passage of all plasma components except RBCs to contact heptaocyte surface. Btw the endothelial cells and hepatocyte is the perisinusoidal space or space of Disse, containing reticular fibers. Kupffer cells (monocyte derivatives) also form part of the fenestrated endothelial layer
Function of Kupffer cells
Play a crucial role in defense, as well as in general removal of particulate material from the blood. They phagocytose particulate materials
7. Describe the walls of the bile canaliculi, canals of Hering and interlobular bile ducts.
Bile canaliculi form from apposed grooves in adjacent hepatocytes, forming a circumferential belt around each hepatocyte and a network of tubules entirely within hepatocyte plates. Hepatocyte plasma membranes and tight junctions prevent leakage of bile, but near portal triad bile canaliculi lead to bile ductules (Canals of Hering) which lead to interlobular bile ducts of portal triad. These fuse to form lobar ducts which then form common hepatic duct.
Epithelial lining of bile ducts
Bile canaliculi walls are composed of adjacent hepatocyte apical plasma membranes. Canals of Hering have cuboidal epithelium with microvillar projections, interlobular bile ducts have initially cuboidal then columnar epithelium as the ducts fuse to form lobar ducts.
Components of bile
Cholate derivatives, cholesterol, phospholipids, bilirubin glucuronides, and proteins, electrolytes, IgA
What connects the gallbladder to the common hepatic duct
The cystic duct connects gallbladder to the common hepatic duct. These fuse to form common bile duct which joins the pancreatic duct and enters duodenum. The sphincter choledochus/sphincter of boyden is located before the entry of the pancreatic duct and the sphincter of Oddi is located after entry of pancreatic duct
8. Be able to describe the mucosa of the gall bladder and explain what it does and what is unique about its structure as compared to other digestive mucosae.
Columnar epithelium has microvilli and tight junctions at apical surface, numerous mitochondria, and folded basolateral surfaces which actively transport salt into basolateral spaces btw cells and water follows where it is taken up by capillaries.
describe blood flow through the liver
Deoxygenated blood from portal vein and oxygenated blood from hepatic artery enter liver > interlobar vessels ( one for vein and one for artery) > interlobular vessels (oxygenated and deoxygenated blood mix via capillaries) > perilobular veins and arteries (connect via capillaries) > inlet venules > sinusoids > central vein > sublobar (intercalated) vein > collecting vein > hepatic veins > inferior vena cava
3. Explain the relationship between the structure of the sinusoidal surface of the hepatocyte and the blood, and the relevance of this to different hepatocyte functions.
The liver acinus has 3 zones. 1: zone of permanent activity which is first to receive oxygen and nutrients and the first to regenerate in liver disease, b/c it is closest to the sinusoids. 2: zone of varying activity. 3: zone around the central vein, the zone of permanent repose which receives less nutrients and is more prone to damage.
where does bile concentration occur
bile ducts reabsorb water and electrolytes to start the process