Lymphoma

Question 1

Burkitt’s immunophenotype and cytogenetics

Answer 1

surface immunoglobulin +
TdT negative
CD 19, 20, 22 +
Most CD 10+

Cytogenetics:
(8;14)
(8;22)
(2;8)

Question 2

Lymphoblastic lymphoma immunobiology

Answer 2

B cell: Tdt positive, CD19, CD79a, or CD22 positive. HLA-DR +

T-cell: cytoplasmic or membrane CD3, TdT+, HLA-DR and CD34 negative

7

Question 3

What are the risk factors for hodgkins lymphoma?

Answer 3

Family Hx
EBV infection
SES- for those with HL <10yrs associated with low SES and crowding, but in young adults associated with high SES and fewer exposures to children
social contacts

Question 4

what are the types of classic HL?

Answer 4

Nodular sclerosing
mixed cellularity
lymphocyte rich
lymphocyte depleted

Question 5

what is the immunophenotype of classic HL

Answer 5

CD30+
CD15+ (70%)
CD45 neg
CD20+ (20-30%)
Pax5+

Question 6

what is the immunophenotype of NLPHL?

Answer 6

CD30 neg
CD15 neg
CD45+
CD20+
CD79a +
Pax5 +

Question 7

what is the definition of constitutional symptoms?

Answer 7

more than 10% weight loss in the last 6mths
drenching night sweats
fever >38C for more than 3 days and unexplained

Question 8

what factors are associated with more of an adverse outcome in HL?

Answer 8

advanced stage
B symptoms
bulk disease
poor response to therapy

Question 9

what does brentuximab target?

Answer 9

CD30

Question 10

what cancer can transform in patients with NLPHL?

Answer 10

DLBCL

Question 11

describe the lugano criteria

Answer 11

1-no uptake above background
2-uptake mediatstinum but liver
5-uptake markedly >liver and or new lesions

1 and 2= CR
3-context dependent
4 and 5= active disease

Question 12

what is the ddx for mediastinal mass?

Answer 12

normal thymus
infection-EBV, atypical mycobacterium, histoplasmosis, toxoplasmosis
lymphoproliferative disease
malignant- HL, Lymphoblastic lymphoma, DLBCL, ALCL, primary mediastinal Bcell lymphoma, germ cell tumour, soft tissue sarcoma, lymph node met from other sites

Question 13

what translocation is associated with ALCL?

Answer 13

t(2;5)

NPM-ALK

Question 14

what translocations are associated with Burkitts lymphoma

Answer 14

8;14-most common
2;8
8;22

Question 15

what is the immunophenotype of DLBCL?

Answer 15

sIg+, light chain restricted
CD19, CD20, CD22, CD79a, PAX-5 +
Can be CD30+
2/3 express BCL-6
1/3 express cMYC

Question 16

BCL-6 is expressed by which NHL, and what can it be used to differentiate sometimes between?

Answer 16

BCL-6 is expressed by DLBCL but not by Burkitts

Question 17

Which NHL is least likely to be associated with EBV?

Answer 17

ALCL