Macrophage / Mononuclear Cell

Question 1

Charactestic

Answer 1

• Dominant cell in chronic inflammation
• driver from circulatory blood , in tissue = macrophage

Question 2

Names

Answer 2

1. Bone: osteoclasta
2. Liver: kupffer cell
3. CNS: microglial cell
4. Lymph node, spleen: sinus histocyte
5. Lung: alveolar macrophage, in ruminant: pulmonary intravascular macrophage

Question 3

Comprise

Answer 3

• mononuclear phagocyte system
• old name: reticulo endothelium system

Question 4

Function, act as:

Answer 4

• filtration particular matter, microbe, senescent cell.
• act a sentinels to alert specific components of adaptive immune ( T,B lymphocytes ) to injurious stimuli.

Question 4

Half life of circulatory monocyte about ________, under influence ________ and _________ they migrate to injury site within _________ after _____________

Answer 4

1 day
Adhesion molecule, chemotactic factor
24-48 hours
Onset of acute inflammation

Question 5

Monocyte reach extravascular tissue and transformation to __________ which has __________ and greater __________ than monocyte

Answer 5

Large macrophage
Longer half life
Capacity for phagocytosis

Question 6

Activated macrophage, result in:

Answer 6

1. Increase cell size
2. Increase metabolism
3. Greater ability to kill ingested microbe
4. Increase content of lysosomal enzyme

Question 7

Light microscope, macrophage appear:

Answer 7

• large
• abundant and clear cytoplasm, pink
• single oval to polygonal, reniform nuclease, eccentric

Question 8

Activated macrophage can differentiate to

Answer 8

Epitheliod macrophage —-> multinucleated giant cell

Question 9

Activation signal for activated macrophage:

Answer 9

1. Bacterial endotoxin and product of microbial.
2. Cytokines produced by T lymphocyte ( IFN-γ ).
3. ECM proteins.
4. Mediator produced during acute inflammation.

Question 10

After activation, macrophage:

Answer 10

Secrete active product resulting in tissue injury and fibrosis
Product: AA,NO,ROS metabolites , cytokines, growth factor, acid and natural proteases, plasminogen activator

Question 11

Epitheliod cell :
- shape

Answer 11

• large than activated macrophage.
• abundant cytoplasm
• cell membrane polygonal to elongated
• forming sheets resemble squamous epithelial

Question 12

Epitheliod cell :
- function

Answer 12

• diminished phagocytosis capacity but contain large amount of Golgi, RER, vesicle and vacuoles.
• has extracellular secretion.

Question 13

Multinucleated giant cell

Answer 13

• syncytial cell formed by fusion two or more activated macrophage into large cell have two of more nuclease

Question 14

Nuclei can be distributed in cell in haphazard manner or aggregated in the center of cytoplasm. This called__________

Answer 14

Foreign body multinucleated giant cell

Question 15

Nulcei may arranged in horseshoelike semicircle at periphery this is called________

Answer 15

Langhans type giant cell