Maths formulas

Question 1

Partition co-efficient formula

Answer 1

Kav = (ve-vo)/(vt-vo)
Ve is the elution volume, Vo is the void volume and Vt is the total column volume.

Question 2

Adjusted retention time

Answer 2

t’r = tr-to

Question 3

Resolution of peak separation formula

Answer 3

2(Tr(b) – Tr(a)) / Wa + Wb

Question 4

N formula

Answer 4

N = 16 (tr/w)2

Question 5

HETP formula

Answer 5

L/N

Question 6

For a GC (with a table) N calculation, what must i do first with the retention time

Answer 6

Tr x chart speed

Question 7

How to calculate relative composition?

Answer 7

Peak Area / Response Factor
Add these answers all together
Then divide and x 100 those answers over the added together answers

i.e
Peak 1: 6
Peak 2: 3
Peak 3: 1

total = 10
6/10* 100= 60

Question 8

How to calculate peak area

Answer 8

Peak Height×Peak Width at Half Height

Question 9

Formula for N at half height

Answer 9

5.54((tr/half w)2

Question 10

Ion exchange matrices example

Answer 10

Sepharose -Agarose

Question 11

Anion and cation functional groups

Answer 11

Anion-weak- DEAE,
Cation- strong-Methyl sulphonate

Question 12

Gel filtration matrices example

Answer 12

Biogel
Superose
Sepharose- agarose

Question 13

HIC matrices

Answer 13

Sepharose -Agarose
Fractogel

Question 14

IMAC matrices

Answer 14

Agarose via IDA/NTA

Question 15

Affinity Matrices

Answer 15

Sepharose -Agarose