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1

Coulomb's Law

U=kq1q2/Dr
Where U is the energy needed to pull apart

2

Y axis of Scatchard Plot

% Occupancy/ [Ligand]

3

Complete Gibbs Equation

dG=dG' + RTln(Products_initial)/(Reactants_initial)

Add the standard free energy to the RTln of the initial

4

How do you combine Keqs?

You MULTIPLY them.

5

What sign does the RT have when Gibbs is at equilibrium?

NEGATIVE

6

Gibbs free energy value at Equilibrium (Numerical)

Zero

7

How do you combine dG free energies?

You ADD them.

8

2nd Law of Thermo (Explanation and equation)

S=kb*ln(W)
Universe tends towards max disorder.
[Boltzmann*ln of possible arrangements]

9

Average H bond energy vs Average Covalent energy

H:5-30
Cov: 100+

10

X intercept of Scatchard plot

Maximum Hormone bound Bmax, or Number of binding sites

11

+H and -S

Nonspontaneous at all Temps

12

How can scatchard plot determine if there's cooperativity or allostery?

deviations from linear slopes.

13

dG', Gibbs equation at equilibrium

-RTln(Keq)

14

-H and +S

Spontaneous at ALL temperatures

15

Small Kd means higher or lower affinity?

Higher, because it means you need less ligand to get to the point where 50% is bound.

16

Dissocation constant kd

Inverse of Keq; so [A]*[B]/[AB]

Kd=Koff/Kon

Kd=Concentration when 50%bound to PL complex.

17

-H and -S

Spontaneous only at LOW temperatures. The Low Temp decreases contribution from S.

18

+H and +S

Spontaneous only at HIGH temperatures; High temp increases contribution from S

19

1st Law of Thermodynamics (Explanation and Equation)

Energy is conserved.
dU=q-w

20

Slope of scatchard plot

-Ka

21

Enthalpy (Explanation and equation)

The Heat in a system where energy is related to the pressure and the volume.
H=U+PV