MCP

Question 1

Coulomb’s Law

Answer 1

U=kq1q2/Dr

Where U is the energy needed to pull apart

Question 2

Y axis of Scatchard Plot

Answer 2

% Occupancy/ [Ligand]

Question 3

Complete Gibbs Equation

Answer 3

dG=dG’ + RTln(Products_initial)/(Reactants_initial)

Add the standard free energy to the RTln of the initial

Question 4

How do you combine Keqs?

Answer 4

You MULTIPLY them.

Question 5

What sign does the RT have when Gibbs is at equilibrium?

Answer 5

NEGATIVE

Question 6

Gibbs free energy value at Equilibrium (Numerical)

Answer 6

Zero

Question 7

How do you combine dG free energies?

Answer 7

You ADD them.

Question 8

2nd Law of Thermo (Explanation and equation)

Answer 8

S=kbln(W)
Universe tends towards max disorder.
[Boltzmannln of possible arrangements]

Question 9

Average H bond energy vs Average Covalent energy

Answer 9

H:5-30
Cov: 100+

Question 10

X intercept of Scatchard plot

Answer 10

Maximum Hormone bound Bmax, or Number of binding sites

Question 11

+H and -S

Answer 11

Nonspontaneous at all Temps

Question 12

How can scatchard plot determine if there’s cooperativity or allostery?

Answer 12

deviations from linear slopes.

Question 13

dG’, Gibbs equation at equilibrium

Answer 13

-RTln(Keq)

Question 14

-H and +S

Answer 14

Spontaneous at ALL temperatures

Question 15

Small Kd means higher or lower affinity?

Answer 15

Higher, because it means you need less ligand to get to the point where 50% is bound.

Question 16

Dissocation constant kd

Answer 16

Inverse of Keq; so [A]*[B]/[AB]

Kd=Koff/Kon

Kd=Concentration when 50%bound to PL complex.

Question 17

-H and -S

Answer 17

Spontaneous only at LOW temperatures. The Low Temp decreases contribution from S.

Question 18

+H and +S

Answer 18

Spontaneous only at HIGH temperatures; High temp increases contribution from S

Question 19

1st Law of Thermodynamics (Explanation and Equation)

Answer 19

Energy is conserved.

dU=q-w

Question 20

Slope of scatchard plot

Answer 20

-Ka

Question 21

Enthalpy (Explanation and equation)

Answer 21

The Heat in a system where energy is related to the pressure and the volume.
H=U+PV