Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Bacte Lec > Method of Identifying Microorganism > Flashcards

Method of Identifying Microorganism Flashcards

(87 cards)

Start Studying
1
Q

Study organisms in their living or natural state
Demonstrates motility

A

Direct Method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Types of Direct Method

A

Wet mount method
Hanging Drop method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Parts of Hanging drop method

A

Depreession (well) slide
Cover slip
Petroleum Jelly (seal)
Drop of liquid with specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Examine organisms in their fixed or stained
state

A

Indirect Method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Types of Indirect Method

A

Smear preparation
Staining method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Specimen for smear preparation

A

Solid specimen
Liquid specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What to do with liquid specimen in smear preparation

A

air dry
heat fix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Attach or secure microorganisms on the glass slide
Kill the microorganism

A

Heat Fix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

artificial coloring of the smear or microorganism
with dyes.

A

Staining Method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Make organism more visible
For identification and classification of
microorganisms
To emphasize special bacterial structure

A

Staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

2 types of staining

A

Simple staining
Differential staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Utilizes only one dye or stain

A

Simple staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Employs 2 dyes

A

Differential staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Stains in differential staining

A

Gram Stain
Acid Fast Stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Components of Gram Stain

A

Crystal Violet
Grams Iodine
Acid Alcohol
Safranin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Color of Gram Negative

A

Pink or Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Color of Gram positive

A

Purple or Blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

All cocci are gram (+) except?

A

Neisseria
Branhamella
Veilonella
Moraxella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

All Bacilli are gram (-) except?

A

Bacillus
Lactobacillus
Listeria
Actinomyces
Corynobacterium
Clostridium
Mycobacterium
Erysipelothrix
Nocardia spp

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Theories of Gram Staining

A

Mg RNA
Kaplan-Kaplann
Stearn-Stearn

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

❖Gram (+) organism’s cell wall contains Mg RNA
❖ Gram (-) organisms cell wall do not contain Mg RNA

A

Mg RNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

❖ Gram (+) organisms are less permeable to decolorizer
❖ Gram (-) organisms easily decolorized by alcohol

A

Kaplan- Kaplan

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

❖ Gram (+) organisms have lower isoelectric point making them more
acidic
❖ Gram (-) organisms have higher isoelectric point making them more
basic

A

Stearn- Stearn

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Gram stain cannot stain?

A

Mycobacteria
Mycoplasma
Rickettsiae
Chlamydia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Components of Acid Fast Stain
Carbol Fuchsin Acid Alcohol Methylene Blue
26
2 methods of Acid Fast stain
Zhiel-Neelsen Method Kinyoun Method
27
Acid fast positive color
Red
28
Acid Fast negative
Blue
29
active detergent or surfactant in acid fast staining
Tergitol
30
artificially prepared material that provides the nutritional and environmental requirements for bacterial growth
Culture media
31
Basic Ingredients of culture media
Beef Extract Peptones Salts Water Agar
32
Types of Culture media state
Solid Liquid Semi-solid
33
With solidifying agent. It provides firm surface
Solid media
34
No solidifying agent. Remains in liquid form
Liquid media
35
Consistency of solid and liquid. Gelatinous consistensy. To demonstrate motility
Semi-Solid media
36
how many percent of agar in solid media
2-3%
37
How many percent of agar in semi-solid media
0.5-1%
38
contains both a solid part and a liquid part in a single bottle
BIPHASIC
39
Composition of culture media
Synthetic/Defined Non-Synthetic/Undefined
40
Method of Dispensing Culture media
Plated Tubed
41
Exact composition of the media is known
Synthetic/Defined
42
Exact composition of the media is unknown/ Raw material
Non-Synthetic/Undefined
43
Agar temperature at room temp
22-25C
44
Agar temperature at incubation
37C
45
Agar temperature at boiling
100C
46
Invented Gram Stain
Hans Christian Gram
47
Thick; Multilayer
Gram +
48
Thinner; Single layer
Gram -
49
Bacteria with darting motiliity
Campylobacteria
50
Bacteria with rapid darting/shooting star
Vibrio cholerae
51
Bacteria with gliding motility
Capnocytophagia
52
Bacteria with swaming motility
Proteus
53
Bacteria with tumbling motility
Listeria
54
Bacteria with twitching motility
Kingella
55
Emulsify is also known as
Round Coiled Type
56
pH change that carries no electrical charge
isoelectric
57
allows passages of liquid or gas
permeable
58
Zhiel-Neelsen method 1. Stains 2. Color of AF and NAF
Carbol Fuchsin Steam Acid Alcohol Methylene Blue RED RED RED RED RED RED COLORLESS BLUE
59
Kinyoun method 1. Stains 2. Color of AF and NAF
Carbol Fuchsin Phenol (10%) Acid Alcohol Methylene Blue RED RED RED RED RED RED COLORLESS BLUE
60
General bacterial with Mycolic Acid
Mycobacterium Nocardia Rhodococcus Gordonia Tsukamurella Corynebacterium
61
Partially digested proteins, carbohydrates in the medium
Peptones
62
Maintain osmotic pressure in the medium
Salts
63
Maintains the pH of the medium
Water
64
Cannot be degraded by microorganisms
Agar
65
Process of introducing microorganism in the culture medium
INOCULATION
66
Sample of microorganism to be inoculated
INOCULUM
67
Prevention of microbial contamination
ASEPSIS
67
the state of being free from disease- causing contaminants
ASEPSIS
68
using flame ( inoculating loop/ needle)
Sterilization
69
passing the test tube mouth/ petri dish over the flame
Sterilization
70
Streaking pattern
Quadrant Streak Continuous Streak Radiant Streak
71
maintain environmental condition
INCUBATION
72
hours incubation period
18-24
73
Optimum temperature of incubation
35-37C
74
Humidity of Incubation
70% or greater No drying or desiccation
75
O2 and CO2 Requirements
Anaerobic incubation
76
Jar used in anaerobiosis
Gaspak Jar
77
Ground meat media overlaid or sealed with petrolatum to prevent the entry of air
Cooked Meat
78
Acts as reducing agents
Mixture of Pyrogallol, KOH, and Sodium Bicarbonate
79
Aids in the initiation and growth of small inoculum and anaerobes by impeding the diffusion of oxygen into the medium.
Thioglycollate media
80
Growth of microorganisms in a culture media
Culture
81
Types of Culture
Pure Culture Mixed Culture
82
Macroscopic growth seen in a culture media
Colony
83
Culture that is less contaminated
Pure culture
84
Culture that is erroneous or no identification
Mixed Culture
85
hydrogen catalytically combines with oxygen in the air to form water
ANAEROBIC JARS
86
Techniques of Anaerobic Jars
Evacuation replacement technique Disposable gas generator envelope

Bacte Lec (4 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions