Methods

Question 0

What is the role of MgCl2 in PCR

Answer 0

Mg bind single nucleotide and acts as a cofactors for DNA polymerase.
Too much MG stabilizes dsDNA and prevents desaturation of strands.

Question 1

Reagents needed in PCR

Answer 1

NF H20
Buffer
dNTP mix
MgCl2
Primer
Primer
Taq pol

Question 2

What is the role of ethidium bromide in gel electrophoresis

Answer 2

Large flat basic molecule that can insert in DNA strand. It forms flourescent complexes with the nucleic acids and can be visualized under UV light

Question 3

What is the loading buffer made up of

Answer 3

Sucrose - makes sample dense and allows it to sink into well

Bromophenol blue - gives color for visualisation. Migrates at roughly 50bp

Question 4

Culture media contains

Answer 4

Water
NaCl and other electrolytes
Peptone
Meat/yeast extract
Blood

Question 5

Methods to detects antibody

Answer 5

ELISA
Agglutination
Precipitation
Immunoflourescence
Complement fixation

Question 6

What compounds absorb UV

Answer 6

Conjugated organic compounds

Question 7

Forward scatter in FACS correlates to..

Answer 7

Cell size

Question 8

Side scatter in FACS correlates to..

Answer 8

Typo plasmid granules
Membrane roughness
Nucleus shape