Micro

Question 1

Difference between M.gordonae and M. Scrofulaceum

Answer 1

Both: Schotochromogen (pigmented in light and dark)

Diff: urease and tween80 hydrolysis
Mg= -,+
MS= +,-

Question 2

Major cause of neonatal meningitis

Answer 2

Listeria monocytogenes

Question 3

MOT of L. Monocytogenes

Answer 3

Ingestion of contaminated food (meat and dairy products)

Question 4

Pulmonary infections in patients with AIDS

Answer 4

Mycobacterium avium complex (MAC)

Question 5

Colonies are white, opaque, and may resemble a “molar tooth”

Answer 5

Actinomyces israelii

Question 6

Causes primary typical pneumonia and walking pneumonia

Answer 6

Mycoplasma pneumoniae

Question 7

Gold standard test for Rickettsial antibodies

Answer 7

Indirect Fluorescent Assays (IFA)

Micro-IF assay

Question 8

Binds to cellulose and chitin present in fungal cell wall

Answer 8

CALCOFLUOR WHITE

Question 9

Fluoresces when exposed to UV light

Answer 9

Calcofluor white

Question 10

Necessary to PRESERVE EASILY DISRUPTED CONIDIAL STRUCTURES in their original relationships

Answer 10

SLIDE CULTURE

Question 11

Major changes that result in novel viral antigens

Answer 11

Antigenic SHIFT

Question 12

Minor changes that occur infrequently

Answer 12

Antigenic DRIFT

Question 13

Major cause of diarrhea in infants

Answer 13

Rotavirus

Question 14

Specimens used for detection of Rotavirus

Answer 14

Stool and rectal swabs

Stool is preferred because of the potential for decreased viral recovery from a small sample size

Question 15

Assay used to detect rotavirus antibody and for serotyping

Answer 15

EIA

Question 16

Assays used for detection of group A rotavirus antigen in fecal samples

Answer 16

EIA and latex agglutination

Question 17

Method that can detect rotavirus RNA

Answer 17

RT-PCR

Question 18

Organism that gives off a “Horse-stable” odor in culture?:

Answer 18

Clostridium dificile

Question 19

Organism that gives off a “bleach-like” odor in culture?

Answer 19

Eikenella

Question 20

Mucoid, pink colonies on plate; produces gas; indole (+). On TSI tube you see yellow on the slant and yellow in the deep. What organism is this?

Answer 20

Klebsiella oxytoca