Micro 6 (Test 2)

Question 1

The class of _____ are difficult to stain due to their thick, waxy capsule.

Answer 1

mycobacteria

Question 2

The _____ stain is designed to penetrate the waxy capsule of mycobacterium.

Answer 2

acid fast

Question 3

All species of mycobacterium are long, thin _____.

Answer 3

rods

Question 4

_____ is the primary dye in the acid-fast stain.

Answer 4

carbol fuschin

Question 5

Place in the correct sequential order the steps in the acid-fast staining process.

a) application of acid alcohol
b) apply heat for at least ten minutes
c) apply carbol fuchsin
d) apply methylene blue

Answer 5

apply carbol fuchsin
apply heat for at least ten minutes
application of acid alcohol
apply methylene blue

Question 6

_____ is the counter stain for the acid-fat stain

Answer 6

methylene blue

Question 7

The _____ acts as the decolorizer in the acid-fast stain.

Answer 7

acid alcohol

Question 8

_____ acts as the mordant in the acid-fast stain.

Answer 8

heat

Question 9

Acid-fast positive organisms will stain _____

Answer 9

red

Question 10

Acid-fast negative organisms will stain _____

Answer 10

blue

Question 11

The acid-fast stain is only for _____ to determine if they are mycobacteria

Answer 11

bacillus

Question 12

A _____ is any material from which bacteria can extract nutrition or any material upon which bacteria will grow.

Answer 12

media/medium

Question 13

A _____ is the general name for any population of bacteria.

Answer 13

culture

Question 14

Media can be in both a _____ and a _____ form.

Answer 14

solid/liquid

Question 15

An example of a solid media is _____

Answer 15

agar

Question 16

A _____ is a form of liquid media.

Answer 16

broth

Question 17

A _____ is a visible, isolated mass of identical bacteria, if they-re not identical then it’s a _____

Answer 17

colony … culture

Question 18

_____ can utilize oxidation of chemicals for their energy supply.

Answer 18

chemotrophs

Question 19

_____ require light in order to metabolize

Answer 19

phototrophs

Question 20

_____ can metabolize off an artificial light source

Answer 20

lumitrophs

Question 21

_____ require sunlight in order to metabolize

Answer 21

solotrophs

Question 22

_____ require from an organic source, carbon

Answer 22

heterotrophs

Question 23

_____ has the metabolic ability to get its required carbon from atmospheric carbon dioxide.

Answer 23

autotrophs

Question 24

TRUE or FALSE: The only evidence of bacterial life is their ability to reproduce

Answer 24

TRUE

Question 25

An _____ is the transfer of bacteria from one media to another media

Answer 25

innoculation

Question 26

The entire population of bacteria in the colony originated from a _____

Answer 26

single cell

Question 27

A _____/_____ is a medium in which the exact quantity and identity of every substance is known.

Answer 27

simple/growth media

Question 28

_____ medium in which some natural product is added to a simple media that results in enhanced growth properties.

Answer 28

enriched

Question 29

_____ bacteria will not grow on a simple medium

Answer 29

fastideous

Question 30

A _____ medium will only allow a select category of bacteria to grow on it and prevent growth of all others

Answer 30

selective

Question 31

A ____ medium is a medium in which all bacteria will grow on it except a certain category will have very expressive characteristics that aren’t normally present

Answer 31

differential

Question 32

We share a symbiotic relationship with _____ bacteria that are located in our large intestines.

Answer 32

enteric (normal flora)