Micro Packet 3 Flashcards

(62 cards)

1
Q

4 Growth requirements of bacteria

A

1) Temperature
2) Gas O2 REquirements
3) Nutriential
4) PH 6.5-7.5

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2
Q

Human blood pH

A

7.35-7.45

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3
Q

Destroys all bacteria

A

Thermal Depth Point

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4
Q

How many thermal death points are there for spores, and what are they for?

A

Two.

One for the vegetative form and one for the spore.

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5
Q

What temperature is best suited for growth?

A

Optimum temperature

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6
Q

Bacteria are classified according to…

A

growth at different temperatures

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7
Q

Cold loving bacteria

A

Psychrophilic

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8
Q

Medium temperature loving bacteria

A

Mesophilic

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9
Q

Heat loving bacteria

A

Thermophilic

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10
Q

Salt loving bacteria

A

Halophilic

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11
Q

Example of halophilic bacteria

A

Staphylococcus

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12
Q

Oxygen loving bacteria

A

Aerobes

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13
Q

CO2 loving bacteria

A

Anaerobes

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14
Q

Means Strict or only

A

Obligate

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15
Q

Means fundamentally tolerating. The gases can be present or absent.

A

Facultative

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16
Q

Grow only in the presence of O2

A

Obligate Aerobe

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17
Q

Grow only in the presence of CO2

A

Obligate Anaerobe

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18
Q

Fundamentally an anaerobe, but can grow in the presence of oxygen.

A

Facultative aerobe

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19
Q

Fundamentally an aerobe, but can grow in the absence of O2. Uses or tolerates a lack of O2.

A

Facultative Anaerobe

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20
Q

Types of Cultures:

A

Agar Plates
Agar slants
Broth (liquid)
Stabbs (Gelatin)

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21
Q

Used to study culture characters for growth patterns

A

Stabbs Culture

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22
Q

Types of media:

A
Complex
Reducing
Selective
Differential
Enrichment
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23
Q

Has extracts: Beef, yeasts, plants, blood; grows aerobes.

A

Complex Media

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24
Q

Has chemicals in it that combine to O2 to deplete the O2 in the media.

A

Reducing Media

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25
A broth used to cultivate obligate anaerobes.
Thioglycollate Media
26
Thioglycollate Media is an example of what type of media?
Reducing Media
27
Has chemicals to inhibit some types of bacteria while allowing other types to grow.
Selective Media
28
Examples of selective media:
Brilliant Green Agar | Chocolate Agar
29
Isolates Salmonella
Brilliant Green Agar
30
Grows Neisseria
Chocolate Agar
31
Contains special sugars that differentiate bacteria, used for identification.
Differential Media
32
Grows only Gram Negative organisms
Endo Agar
33
Contains the sugar Lactose (pink color)
Endo Agar
34
If lactose is fermented, it's colors turns to...
dark purple
35
Has the sugar Mannitol in it, the media is a pink color, has high salt concentrate.
Mannitol Salt Agar
36
Mannitol Salt Agar is _________ + __________ .
Selective and Differential
37
Only _________ bacteria can grow in a high concentration of salt.
Halophilic
38
Mannitol Salt Agar is _______ .
Halophilic
39
All ________ are halophilic
Staphylococci
40
Only one species of staph ferments mannitol. What is it?
Staph Aureus
41
When sugar is fermented, what is produced?
An acid-waste
42
Acid-waste product of Staph Aureus turns the indicator color in the media from pink to...
Yellow
43
Additives are placed in media which allows organisms to grow which would not be able to survive.
Enrichment Media
44
An example of an organism grown in a candle jar is...
Neisseria
45
A candle is lit, O2 is reduced.
Candle Jar (growing anaerobes).
46
Contains a plastic jar with palladium catalyst. Reagents placed in the system will yield Hydrogen and CO2. Indicator strip is placed in the jar.
Gas Pak System
47
When the indicator strip is blue, there is _________ present in the jar.
O2
48
When the indicator strip is white or colorless, ____________ conditions are present.
Anaerobic
49
All Bacteria Grow
Blood Agar
50
All bacteria grow.
Nutrient Agar
51
Only Gram positive bacteria grow
Pea Agar
52
Only Gram Negative bacteria grow
Endo Agar
53
Bacteria reproduce by _______ fission or ________ fission.
Binary, Transverse
54
Division into two equal parts
Binary or Transverse fission
55
Time required for a cell to divide is called...
division times or generation time
56
4 phases of bacterial growth
LAG LOG Stationary Death
57
No reproduction, zero growth, a period of adjustment
LAG Phase
58
Time of active metabolism, growth occurs at maximum rate, very sensitive to antibiotics at this time. (Also known as Exponential Phase)
LOG Phase
59
This is where growth=death. Number of organisms growing equals the number of organisms dying.
Stationary Phase
60
Organisms are dying rapidly.
Death or Decline Phase
61
The _______ + _________ phases show a logarithmic change in numbers.
Log + Death
62
Not all bacteria have the same generation time. It can be altered by environment. Cells do not reach the sense point at the same time.
Transitional Phase.