Micro Packet 3

Question 1

4 Growth requirements of bacteria

Answer 1

1) Temperature
2) Gas O2 REquirements
3) Nutriential
4) PH 6.5-7.5

Question 2

Human blood pH

Answer 2

7.35-7.45

Question 3

Destroys all bacteria

Answer 3

Thermal Depth Point

Question 4

How many thermal death points are there for spores, and what are they for?

Answer 4

Two.

One for the vegetative form and one for the spore.

Question 5

What temperature is best suited for growth?

Answer 5

Optimum temperature

Question 6

Bacteria are classified according to…

Answer 6

growth at different temperatures

Question 7

Cold loving bacteria

Answer 7

Psychrophilic

Question 8

Medium temperature loving bacteria

Answer 8

Mesophilic

Question 9

Heat loving bacteria

Answer 9

Thermophilic

Question 10

Salt loving bacteria

Answer 10

Halophilic

Question 11

Example of halophilic bacteria

Answer 11

Staphylococcus

Question 12

Oxygen loving bacteria

Answer 12

Aerobes

Question 13

CO2 loving bacteria

Answer 13

Anaerobes

Question 14

Means Strict or only

Answer 14

Obligate

Question 15

Means fundamentally tolerating. The gases can be present or absent.

Answer 15

Facultative

Question 16

Grow only in the presence of O2

Answer 16

Obligate Aerobe

Question 17

Grow only in the presence of CO2

Answer 17

Obligate Anaerobe

Question 18

Fundamentally an anaerobe, but can grow in the presence of oxygen.

Answer 18

Facultative aerobe

Question 19

Fundamentally an aerobe, but can grow in the absence of O2. Uses or tolerates a lack of O2.

Answer 19

Facultative Anaerobe

Question 20

Types of Cultures:

Answer 20

Agar Plates
Agar slants
Broth (liquid)
Stabbs (Gelatin)

Question 21

Used to study culture characters for growth patterns

Answer 21

Stabbs Culture

Question 22

Types of media:

Answer 22

Complex
Reducing
Selective
Differential
Enrichment

Question 23

Has extracts: Beef, yeasts, plants, blood; grows aerobes.

Answer 23

Complex Media

Question 24

Has chemicals in it that combine to O2 to deplete the O2 in the media.

Answer 24

Reducing Media

Question 25

A broth used to cultivate obligate anaerobes.

Answer 25

Thioglycollate Media

Question 26

Thioglycollate Media is an example of what type of media?

Answer 26

Reducing Media

Question 27

Has chemicals to inhibit some types of bacteria while allowing other types to grow.

Answer 27

Selective Media

Question 28

Examples of selective media:

Answer 28

Brilliant Green Agar | Chocolate Agar

Question 29

Isolates Salmonella

Answer 29

Brilliant Green Agar

Question 30

Grows Neisseria

Answer 30

Chocolate Agar

Question 31

Contains special sugars that differentiate bacteria, used for identification.

Answer 31

Differential Media

Question 32

Grows only Gram Negative organisms

Answer 32

Endo Agar

Question 33

Contains the sugar Lactose (pink color)

Answer 33

Endo Agar

Question 34

If lactose is fermented, it's colors turns to...

Answer 34

dark purple

Question 35

Has the sugar Mannitol in it, the media is a pink color, has high salt concentrate.

Answer 35

Mannitol Salt Agar

Question 36

Mannitol Salt Agar is _________ + __________ .

Answer 36

Selective and Differential

Question 37

Only _________ bacteria can grow in a high concentration of salt.

Answer 37

Halophilic

Question 38

Mannitol Salt Agar is _______ .

Answer 38

Halophilic

Question 39

All ________ are halophilic

Answer 39

Staphylococci

Question 40

Only one species of staph ferments mannitol. What is it?

Answer 40

Staph Aureus

Question 41

When sugar is fermented, what is produced?

Answer 41

An acid-waste

Question 42

Acid-waste product of Staph Aureus turns the indicator color in the media from pink to...

Answer 42

Yellow

Question 43

Additives are placed in media which allows organisms to grow which would not be able to survive.

Answer 43

Enrichment Media

Question 44

An example of an organism grown in a candle jar is...

Answer 44

Neisseria

Question 45

A candle is lit, O2 is reduced.

Answer 45

Candle Jar (growing anaerobes).

Question 46

Contains a plastic jar with palladium catalyst. Reagents placed in the system will yield Hydrogen and CO2. Indicator strip is placed in the jar.

Answer 46

Gas Pak System

Question 47

When the indicator strip is blue, there is _________ present in the jar.

Answer 47

O2

Question 48

When the indicator strip is white or colorless, ____________ conditions are present.

Answer 48

Anaerobic

Question 49

All Bacteria Grow

Answer 49

Blood Agar

Question 50

All bacteria grow.

Answer 50

Nutrient Agar

Question 51

Only Gram positive bacteria grow

Answer 51

Pea Agar

Question 52

Only Gram Negative bacteria grow

Answer 52

Endo Agar

Question 53

Bacteria reproduce by _______ fission or ________ fission.

Answer 53

Binary, Transverse

Question 54

Division into two equal parts

Answer 54

Binary or Transverse fission

Question 55

Time required for a cell to divide is called...

Answer 55

division times or generation time

Question 56

4 phases of bacterial growth

Answer 56

LAG LOG Stationary Death

Question 57

No reproduction, zero growth, a period of adjustment

Answer 57

LAG Phase

Question 58

Time of active metabolism, growth occurs at maximum rate, very sensitive to antibiotics at this time. (Also known as Exponential Phase)

Answer 58

LOG Phase

Question 59

This is where growth=death. Number of organisms growing equals the number of organisms dying.

Answer 59

Stationary Phase

Question 60

Organisms are dying rapidly.

Answer 60

Death or Decline Phase

Question 61

The _______ + _________ phases show a logarithmic change in numbers.

Answer 61

Log + Death

Question 62

Not all bacteria have the same generation time. It can be altered by environment. Cells do not reach the sense point at the same time.

Answer 62

Transitional Phase.