Micro Packet 4 Flashcards
(41 cards)
To reduce or eliminate bacterial growth depends on…
number, species, temperature, pH & organic matter
Bacterial Death can result from damage to…
1) Cell Wall
2) Cell Membrane
3) Proteins
4) Nucleic Acid (DNA/RNA)
_____________ denatures enzymes.
Heat
An example of moist heat is the…
Autoclave
Autoclave is heat under…
Pressure
Autoclave kills bacteria & spores at ______ psi + _______ for 15 minutes.
15 lbs psi + 121 c
Kills bacteria and spores
Inceration
Example of dry heat
flaming the loops
Best for heat labile material.
Filtration
Causes death of mutations and there are two types.
Radiation
Two types of radiation
Ionizing
Ultraviolet
Kills bacteria
Bactericidal
Radiation such as gamma or x-ray
Ionizing
This type of radiation cannot be used to sterilize heat labile solutions in plastic, and is used in the medical and dental supply industries.
Ionizing
Low penetrating power
Ultraviolet Radiation
To kill, it must be exposed directly to the organism
Ultraviolet radiation
Solids like paper, glass and plastic will protect the organism by covering it.
Ultraviolet radiation
Two chemical methods of sterilization…
Antiseptics
Disinfectants
Prevent growth or arrest the development of the microbes
Antiseptics
Chemicals that kill bacteria (Ex: halogens, heavy metals, acids, organic compounds & dyes)
Disinfectants
__________ + ___________ denature proteins.
Phenols + Phenolics
Have a detergent like affect on the cell membrane
Phenols + Phenolics
Can be added to soaps to increase cleansing properties
Phenols + Phenolics
These are an active ingredient in mouthwash + sore throat preps.
Phenols + Phenolics