microbiology

Question 1

• allow room air to pass into cabinet & around the area & material within, sterilizing only the air to be exhausted
• consist of negative pressure
• operated in open front

Answer 1

class 1

Question 2

• air flows in ‘sheets’, serves as barrier to partcile from outside the cabinet
• • also called VERTICAL LAMINAR FLOW BSCs

Answer 2

class II

Question 3

self-contained, 70% of air is recirculated

Answer 3

class IIA

Question 4

selected for radioisotopes, toxic chem/ carcinogenic samples

Answer 4

class IIB

Question 5

Prokaryotic unicellular organisms that lack a
true nucleus and nuclear membrane

Answer 5

bacteria

Question 6

• Single, closed, circular chromosomes Of
  double-stranded DNA called NUCLEOID

Answer 6

bacteria

Question 7

small circular molecules of
extrachromosomal circular DNA

Answer 7

plasmids

Question 8

asexual reproduction of
bacteria

Answer 8

binary fission

Question 9

bacteria size

Answer 9

range from 0.2 to 2pm in
diameter and 1 to 6prn in length

Question 10

• Principal stain used for microscopic
  examination Of bacteria

Answer 10

gram stain

Question 11

• First devised by Hans Christian Gram during
  the late 19th century

Answer 11

gram stain

Question 12

: take up the basic dye, crystal
violet

Answer 12

gram-positive

Question 13

allow crystal violet dye to wash
out easily with decolorizer alcohol or acetone

Answer 13

gram-negative

Question 14

cell wall: thick (peptidoglycan)
lipopolysaccharide: absence
gram staining: purple
representation: picture of gram-positive (“PURPLE/VIOLET result)

Answer 14

gram-positve

Question 15

cell wall: thin (peptidoglycan)
lipopolysaccharide: presence
gram staining: red/pink
representation: picture of gram-negative (:PINK” result)

Answer 15

gram-negative

Question 16

4 basic morphological types of bacteria

Answer 16

1. COCCI
2. BACILLI
3. SPIRILLA
4. VIBRIOS

Question 17

spherical-shaped cells

Answer 17

cocci

Question 18

rod-shaped cells

Answer 18

baccili

Question 19

spiral shaped cells

Answer 19

spirilla

Question 20

comma- shaped cells

Answer 20

vibrios

Question 21

2 types of bacterial metabolism

Answer 21

1. fermentation
2. respiration

Question 22

Process by which bacteria catabolize
carbohydrates to produce energy

Answer 22

fermentation

Question 23

• also known as oxidation
• Process of bacterial energy generation
  rather than fermentaion

Answer 23

respiration

Question 24

BACTERIA DO NOT HAVE CELL MEMBRANES THEY HAVE CALL WALLS

Question 25

7 sites for collection of various body sites

Answer 25

1. throat (swabs) 2. sputum 3. stool 4. urine 5. blood 6. csf 7. genital tract

Question 26

Expectorated specimens from deep cough should be collected into a sterile specimen cup

Answer 26

sputum

Question 27

* Should be collected aseptically by PHYSICIAN * Should be processed immediately and not exposed to heat or refrigeration

Answer 27

csf

Question 28

MUST NEVER REEFRIGERATED IF IT'S FOR MICROBIOLOGY

Question 29

Support the growth Of a wide range Of microorganisms and are considered non selective because, the growth of most organisms is supported

Answer 29

nutritive media

Question 30

example are blood agar plate & chocolate agar

Answer 30

nutritive media

Question 31

* Microorganisms that can be distinguished on the basis of certain growth

Answer 31

blood agar plate

Question 32

Support the growth of one group of organisms, but not another, by adding antimicrobials, dyes, or alcohol to a particular medium

Answer 32

selective media

Question 33

Example s are MacConkey Agar, Columbia agar with colistin and nalidixic acid (CNA)

Answer 33

selective media

Question 34

* Also called supplemental or enrichment broth * For detection of small numbers of organisms present

Answer 34

backup broth

Question 35

Examples are Thioglycollate (Thio) broth, brain-heart infusion broth (BHIB) and tryptic soy broth (TSB)

Answer 35

backup broth

Question 36

Used when specific organisms are suspected

Answer 36

specialized media

Question 37

* Examples are Mannitol salt agar, bismuth sulfite agar, Campylobacter agar, * Thiosulfate-citrate-bile salts-sucrose (TCBS) agar

Answer 37

specialized media

Question 38

# incubation 35C-37C: Normal incubation temperature for bacterial cultures

Question 39

# incubation Anaerobic jars, bags, or an anaerobic chamber are appropriate for incubation of anaerobic cultures

Question 40

# incubation 42C to 45C: for stool culture for detection of Campylobacter jejuni

Question 41

specimen rejection criteria:

Answer 41

1. Specimen received in nonsterile or contaminated containers 2. Specimen contaminated with barium or other foreign substances 3. 24- hour urine or sputum collections 4. Saliva instead of sputum 5. Unrefrigerated urine specimens 2 hours or more post-collectio