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Flashcards in MICROBIOLOGY Deck (241)
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1

Capsule can be used for

Serotype by swelling

2

To demonstrate capsule, grow the culture on media containing

*milk
*serum

3

The Quellung test depends on the antigenic specificity of the

capsule

4

Dark staining granules

Metachromatic

5

Organisms that vary in size and shape

Pleomorphic

6

In the bacterial growth cycle, growth ceases because nutrients are exhausted or toxic metabolic products have accumulated in the

Stationary/Plateau Phase

7

Bacteria form spores

*Clostridium
*Bacillus

8

Bacteria that grow in the absence of athmospheric (free) oxygen and obtain oxygen from oxygen containing compounds

Anaerobes

9

The temperature at which bacteria grow best

Optimum

10

A slimy colony on blood agar medium indicates which of the following characteristics may be present?

Organism has a capsule

11

On blood agar plates a small zone of alpha-hemolysis surrounded by zone beta hemolysis after refrigeration

Alpha-prime

12

When using fractional sterilization the sequence

Heating (kill vegetative bacteria)
Incubation (spores germinate)
Heating (kills remaining vegetative bacteria)

13

Most effective method of sterilization

Autoclave

14

Used for quality control for the dry heat oven

Bacillus subtilis

15

Filters can give 100% sterility

Millipore (0.22um)

16

Media that cannot be heated can be sterilized by

Filtration

17

Which of the following is not a disinfectant?

70% alcohol

18

Iodophors are composed of

Iodine and Detergent

19

Quarternary ammonium disinfectants are easily inactivated by

Organic Material

20

Term that describes a process or treatment that renders a medical device, instrument or environment surface to handle

Decontamination

21

Gram positive bacteria stain

Purple

22

The mordant used in the Ziehl-Neelsen acid fast stain

Heat

23

Smear for an acid fast stain fixed

Slide warmer at 65C for 2 hours

24

Acid fast bacteria appear what color microscopically

Red

25

Acid fast organism in tissue are best stained by

Kinyoun

26

In the Ziehl-Neelsen staining mtd, the decolorizer

Alcohol + HCl

27

An exmaple of negative stain

India Ink stain

28

NOT gram negative

Peptococcus

29

NOT an anaerobe

Campylobacter

30

If a patient is taking antimicrobials, which can neutralize the antimicrobials

Thiol broth