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Flashcards in MYCOLOGY AND VIROLOGY Deck (127)
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1

Members of the plant kingdom
Lack of roots and stems
Lack chlorophyll

Fungi

2

Coenocytic hyphae

Aseptate hyphae

3

Type of mycoses produce no cellular response by the host

Superficial mycoses

4

Arthroconidia on the outside of the hair as well hyphae within the hair shaft

Ectothrix

5

True yeast produce what in the asexual stage

Blastoconidia

6

Septate hyphae have

Divisions

7

Molds with aseptate hyphae produced a specialized hyphal structure called

Sporangiospore

8

Basic, branching, intertwining structures of molds

Mycelium

9

Arthroconidia are formed

directly from hyphae by fragmentation

10

Mycelia within the colony that grow into the substrate are called

Vegetative hyphae

11

Chlamydospores that grow within the hyphae

Intercalary

12

Budding forms in which the daughter cell forms as a bud from a single mother cell or forms laterally from a mycelium or pseudo mycelium

Blastospores

13

Result of asexual production

Ascospores

14

Conidia are

Asexual spores

15

Macroconidia are usually

Multicellular

16

Swollen portion of the conidiophore is called

Vesicle

17

NOT apply to true yeast

They exhibit thermal dimorphism

18

Conidia with rough spiny surfaces are described as

Echinulate

19

Conidia in chains are said to be

Catenate

20

When preparing temporary mounts of fungal elements, why is the slide gently heated?

Increase the rate of clearing

21

Acts as a clearing agent to eliminate debris and make fungal elements more prominent in skin, hair, nails

10% KOH

22

Stain in Aman medium

Cotton blue

23

In the Periodic Acid Schiff stain (PAS), what colors denote the presence of GLYCOGEN

Red to violet

24

Stain used for delineation of fungal elements by fluorescent microscopy

Calcofluor white

25

When the fungus wall is invinsible with the Hematoxylin and Eosin stain, which of the following may be used to restain the slide

Periodic acid Schiff stain

26

Color of FUNGAL elements in acridine orange staining procedure

Fluoresces a green color

27

How does Mycosel medium differ from Sabouraud dextrose agar

Contains chlorampenicol and cycloheximide

28

Fungus medium used for the primary isolation and maintainance of fungal cultures

Sabouraud Dextrose Agar

29

Indcator in Dermatophyte Test Medium

Phenol Red

30

If 1% glucose is added to cornmeal agar, Trichopython rubrum can be differentiated from Trichophyton mentagrophytes by the production of

Pigment

31

Fungi shoul be incubated at

25-30 C

32

How long should fungus cultures be held before reporting as negative

30 days

33

Antibiotics added to media for the isolation of pathogenic fungi from contaminated material

Cycloheximide and chlorampenicol

34

Media stimulates the production of chlamydospores

Cornmeal agar

35

Media identifies species of aspergillus

Czapek's agar

36

Positive resul in the hair baiting test

V-shaped penetration of the hair shaft

37

Tinea infection is commonly referred to as

Ringworm

38

Organism that cause white piedra

Trichosporon beigelii

39

Tinea unguinum is a dermatophyte infection of the

Nail plates

40

The favus type of Tinea capitis is caused by

Trichophyton schoenleinii

41

Anthrophilic

Microsporon audouni

42

Hairs infected with which of these dermatophytes fluoresce under Wood's lamp

Microsporum canis

43

Colonization by dermatophytic fungi of the hair, nails and skin is called

Dermatophytoses

44

Fungi that cause dermatophytoses

Epidermophyton
Trichophyton
Microsporum

45

Produces macroconidia that are large, multicellular and club shaped with smooth walls

Epidermophyton floccosum

46

NOT true

T. rubrum is noted for its balloon forms

47

Produces small, vey slow growing colonies and has favic chandeliers

Trichophyton schoenleinii

48

Diagnostic for chromoblastomycosis

Sclerotic body

49

Found in yeast phase of Sporothrix schenckii on culture medium at 37 C

Cigar shaped yeast cells

50

Result of an antigen-antibody reaction in cases of sporotrichosis

Asteroid body

51

In older mold cultures of Sporothrix schenckii, single conidia borne along the sides of hyphae are referred as

Sleeve

52

Infections that is caused by dermatiaceous saprobes that invade organs of immunosuppressed host

Phaeohyphomycosis

53

Most common cause of mycetoma (maduramycosis) in the US

Pseudoallescheria boydii

54

Dermatiaceous fungi have colonies with

Surface and reverse side dark

55

Dermatiaceous fungi

Phialophora and Cladosporium

56

Sclerotic bodies are found in tissue in an infection with

Cladosporium

57

NOT cause infection of chromoblastomycosis

Exophiala

58

Fungus that grows as a mold at 35-37 C and at 25 C

Monomorphic

59

True fungi that produce hyphae and spore forms

Eumycotic

60

Causes an infection by hyaline saprobes that invade most tissues or body fluids of immunosuppresed host

Aspergillus
Penicillium marneffei
Fusarium moniliforme

61

If you observe cleistothecia containing ascospores, report organism as

Pseudoallescheria boydii

62

Actinomyces are best classified as

Fungus like bacteria

63

Anaerobic, gram positive but not acid fast and does not stain with fungal stains

Actinomyces israelli

64

Has a capsule

Cryptococcus neoformans

65

When culturing Cryptococcus on Sabouraud dextrose agar, what antibiotics should not be in the medium

Cycloheximide

66

In the direct microscopic examination of CSF, what is mistaken as lymphocyte

Cryptococcus

67

Why does Cryptococcus neoformans pproduce a brown color on Birdseed or staib agar

Assimilates creatinine

68

C. neoformans latex agglutination test on spinal fluid detects cryptococcal

Antigen

69

Candida albicans produces

Blastospores
Chlamydospores
Pseudohyphae

70

Another name of oral candidiasis

Thrush

71

Presumptive identification of Candida albicans can be made by checking the ability of the organism to produce

Germ tube

72

Cornmeal agar plus Tween 80 is used to identify Candida albicans through the organisms production of

Chlamydospores

73

Geotrichum candidum colonies appear as

Rapid, yeast like growth

74

Laboratory workers are in no danger when handling which form of dimorphic fungi

Yeast form (tissue)

75

Sepedonium produces spiny, tuberculate macroconidia but is a

Monomorphic mold

76

Produced by Geotrichum candidum

Rectangular, evenly staining arthroconidia

77

Dimorphic fungi

Histoplasma capsulatum
Blastomyces dermatitidis
Paracoccidioides brasiliensis

78

Tissue phase of Histoplasma capsulatum characteristically shows

Yeast cells within macrophages

79

Characteristic structures of the mycelial phase of Histoplasma capsulatum are

Tuberculate chlamydospores

80

Leishmania can be differentiated from Histoplasma because Leishmania has

Cental nuclear material

81

Test that may be sued instead of conversion when identifying dimorphic fungi

Exoantigen test

82

In the mycelial form, Blastomyces dermatitidis produces

Conidiophores that resemble lollipops

83

A yeast form with a single broad based bud would identify an organism as

Blastomyces

84

The characteristic structures of the mycelial phase of blastomycosis are

Small pear shape conidia called lollipops

85

Yeastlike forms with multiple buds

Paracoccidioides brasiliensis

86

Immature spherules of Coccidioides are differentiated from yeast forms of Blastomyces because

They never bud

87

Fungi that is a major biohazard to laboratory personnel

Coccidiodes immitis

88

Exoantigen test is a

Microscopic immunodiffusion test

89

In the exoantigen test for Histoplasma capsulatum, which bands are present in a positive result

H and/or M

90

When differentiating Histoplasma capsulatum from Leishmania using special fungus stains, Leishmania stains

Will not stain

91

Bands in the Exoantigen test for Blastomyces dermatitidis is present in positive result

A

92

Fungi produces a spherule that is filled with endospores

Coccidiodes immitis

93

Zygomycosis is caused by

Mucor
Rhizopus

94

Fungal elements in zygomycosis show hyphae that are

Broad and nonseptate

95

A mature virus particle containing a nucleic acid surrounded by protein coat, with or without envelope is called a

Virion

96

Viruses are characterized by the presence of

DNA and RNA

97

Largest DNA virus

Poxvirus

98

Smallest RNA virus

Enterovirus

99

First step in the replication of cycle of a virus

Attachment and Penetration

100

The virus capsid morphology is

Helical or icosahedral

101

From what part of the virus is the envelope acquired

Nuclear or cytoplasmic membrane

102

Where is the site of virion assembly

Nucleus or cytoplasm

103

DNA virus

Herpesviridae

104

RNA virus

Pseudomyxoviridae

105

Specimens collected for virus isolation should be kept at

4 C

106

Transport temperature of viruses

- 70 C

107

In what family of viruses, after primary infection, does an individual become latently infected and then the infection can be reactivated

Herpesviridae

108

Cytomegalovirus isolation is best accomplished using

Human emryonic fibroblasts

109

Virus causes acute acute central nervous system disease in humans and animals

Rabies

110

Specimens should be collected from a patient with suspected enteroviral meningitis

Stool
Throat swab
CSF

111

Influenza A viruses can be detected in cell cultures by

Hemadsorption assay

112

The best host system for influenza virus isolation are

Monkey kidney cells
Embryonated hen's egg

113

What common antigen is cross reactive in all human adenoviruses

Hexon

114

NOT characteristics similar to both influenza and parainfluenza

Segmented RNA genome

115

Myxoviridae virus envelope contains both

Neuraminidase and hemagglutinin

116

Eosinophilic cytoplasmic inclusions are elementary bodies of the

Poxvirus

117

Geman Measles is caused by

Rubella virus

118

Mild form of small pox

Alastrim

119

Laboratory procedures that is routinely used for the detection of Hepatitis B virus (HBV) in blood donors

Solid phase RIA
ELISA

120

Most raapid mtd for diagnosis of a virus infection

Direct electron microscopy

121

Specimen collected from a patient with suspected influenza

Throat swab

122

Virus group contains a double stranded, segmented RNA genome

Reovirus

123

Enteroviruses can be differentiared from rhinoviruses by

Acid resistance

124

Virus most frequent cause of gastroenteritis in children during the winter months

Rotavirus

125

NOT resistant to ether treatment

Herpesviruses

126

Most common method for rapid electron microscopy examination is

Negative staining

127

Various virus groups can be differentiated in negatively stained specimens by their

Morphology