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Microbiology 3 Flashcards

Enterobacteriaceae (95 cards)

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1
Q

Nonmotile Enterobacteriaceae

A

Shigella Klebsiella
Y. enterocolitica @37C

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2
Q

Nonmotile @ 37C

A

Y. enterocolitica
Listeria

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3
Q

Biochemically inert

A

Shigella - only Methyl red (+)

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4
Q

“CPON NO GF FA”

A

Enterobacteriaceae

Catalase positive Oxidase negative
Nitrate reducers
Glucose fermenters
Facultative anaerobes

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5
Q

LF enzymes

A

Lactose permease
Beta-galactosidase

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6
Q

RLF + TSI reaction

A

“EKE”
Escherichia Klebsiella Enterobacter
TSI: A/A H2S(-)

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7
Q

LLF + TSI reaction

A

“Cit Ser, Yer Haf Sa Shi is LATE”
Citrobacter
Serratia
Yersinia enterocolitica
Hafnia
Salmonella arizonae
Shigella sonnei

TSI: A or K/A

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8
Q

NLF + TSI reaction

A

“Sarap-Sarap ng PPM Eh!”
Salmonella
Shigella
Proteus
Providencia
Morganella
Edwardsiella

TSI: K/A

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9
Q

Rapid urease producer except

A

Providencia stuartii

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10
Q

PPM

A

Rapid urease producer
LIA: R/A (lysine deamination)
Phenylalanine deaminase (+)
Motile
UTI

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11
Q

TSI K/K

A

Pseudomonas

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12
Q

Most common cause of UTI in human

A

Uropathogenic E. coli (UPEC)

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13
Q

ETEC stool

A

Profuse watery stool
Traveler’s diarrhea/Montezuma’s revenge

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14
Q

Cholera-like toxin

A

ETEC
Heat-labile enterotoxin

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15
Q

Shiga-like toxin

A

EHEC (Verotoxin)

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16
Q

Shigella-like infection

A

EIEC

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17
Q

EIEC stool

A

Explosive watery stool with mucus and blood

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18
Q

EIEC strains

A

Like Shigella: nonmotile, NLF

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19
Q

Shigella stool

A

Scanty, bloody

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20
Q

Watery diarrhea with mucus, no blood (non-invasive, no toxin)
Infantile diarrhea/pediatrics

A

EPEC

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21
Q

Green metallic sheen on EMB

A

E. coli

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22
Q

Associated with E. coli O157:H7

A

EHEC/VTEC

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23
Q

Sorbitol (-) Colorless on SMac
MUG (-)

A

E. coli O157:H7

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24
Q

Most severe manifestation of EHEC

A

HUS

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25
Jack in the Box outbreak - beef hamburger disease Hemorrhagic colitis HUS
E. coli O157:H7
26
Adhere to HEp2 in aggregative "stacked brick pattern"
EAEC
27
Indole positive Klebsiella
K. oxytoca + - ++
28
LDC (+)
Klebsiella, Salmonella
29
Enterobacter slow urease (+)
E. gergoviae
30
Enterobacter produce yellow pigmentation that intensifies at 25C
Enterobacter sakazakii
31
Slow urease producers
"CKEYS" Citrobacter Klebsiella Enterobacter gergoviae Y. enterocolitica Serratia
32
Triple LOA (-)
Pantoea agglomerans
33
Triple LOA (+)
Plesiomonas
34
Triple enzyme (+)
DNAse, Lipase, Gelatinase (+) Serratia
35
E. aerogenes LOA
++-
36
E. cloacae LOA
-++
37
K. pneumoniae LOA
+--
38
E. coli LOA
++-
39
Donovan bodies (pathognomonic) Granuloma inguinale/donovanosis
Klebsiella granulomatis
40
Produce prodigiosin (red pigment) at room temp
Serratia marcescens
41
NLF H2S(+) Motile LDC (+)
Salmonella
42
Pea soup stool (green)
Salmonella
43
(+) Methyl red, ONPG Slow urease (+) Growth on KCN agar
Citrobacter freundii
44
Selective medium for S. typhi
Bismuth Sulfite Agar BSA Black colonies with metallic sheen
45
Milk-borne disease of cow
S. typhi
46
OX-K source
P. mirabilis
47
Shigella ONPG+
S. sonnei
48
Associated with staghorn/struvite/Mg ammonium phosphate stones/triple phosphate crystal
Proteus
49
H2S (+)
"SPACEd" Salmonella Proteus Arizonae Citrobacter freundii Edwardsiella tarda
50
Providencia differentiating test
Citrate (+)
51
Morganella differentiating test
ODC (+)
52
E. tarda differentiating test from E. coli (same IMViC)
H2S+ NLF
53
Vector of Y. pestis
Xenopsylla cheopsis (rat flea)
54
Cauliflower colonies on BAP 48H
Y. pestis
55
Appendicitis-like pain in children is caused by
Y. enterocolitica
56
Stalactite pattern in broth culture
Y. pestis
57
Bull's eye colonies in CIN after 48H
Y. enterocolitica Aeromonas
58
Preferential growth temp of Yersinia
25-30C
59
Motile at 25C, nonmotile at 37C
Yersinia
60
Sulfur source of H2S medium
Sodium thiosulfate
61
Sulfur source of TSI
Ferrous sulfate (nonmetallic)
62
Other H2S media and their H2S indicator
SSA XLD HEA SIM LIA Ferric ammonium citrate (FAC)
63
Media with phenol red as indicator
"MX CUT" MSA XLD CTA Urease TSI
64
NLF nonH2S producer
"SSPPM minus SPACEd" Shigella Providencia Morganella
65
Lysine deaminase positive Phenylalanine deaminase positive
PPM
66
Product of deamination of acids
Ammonia
67
K/A H2S-
Shigella
68
Tests that uses bromthymol blue as indicator
Citrate, Acetate, Acetamide, Malonate
69
Fish-eye colonies on EMB (pink with dark center)
Enterobacter
70
Pink colonies with pink halo on MAC
E. coli
70
XLD inhibitor
Sodium desoxycholate
71
Yellow colonies with black center on XLD
Proteus
72
E. coli on HEA
Salmon orange
73
E. coli on XLD
Yellow with yellow halo
74
Indole test detects
Tryptophanase
75
Indole test indicator
PDAB Kovac's/Ehrlich's reagent
76
Indole positive control
E. coli
77
Spot indole test reagent and positive result
PDAC (+) blue
78
Indole positive Enterobacteriaceae
"May PEKPEC Po?" Morganella Proteus vulgaris E. coli Klebsiella oxytoca Edwardsiella tarda Citrobacter koseri Plesiomonas
79
Methyl red test positive end products are
"LAFS" mixed acid fermentation pathway Lactic acid Acetic acid Formic acid Succinic acid
80
VP reagents
a-naphthol and 40% KOH
81
VP detects
acetoin
82
VP positive
"KEESH" Klebsiella Enterobacter Ewingella Serratia Hafnia
83
Media with bromthymol blue indicator
"CHOT" Citrate HEA OF Hugh Leifson TCBS
84
Citrate positive
"PeRO Sa CHEEKS" Providencia Serratia Citrobacter Hafnia Ewingella Enterobacter Klebsiella Salmonella enteritidis
85
Rapid urease producers
"TPUNCH" T. mentagrophytes PPM Ureaplasma Nocardia C. neoformans H. pylori
86
LOA end products
"Let's Order A CaPuCino" Lysine - Cadaverine Ornithine - Putrescine Arginine - Citrulline
87
Medium where all Salmonella will grow except S. typhii
Brilliant green agar
88
Shigella group types
"DA FB BC SD" S. dysenteriae - A S. flexneri - B S. boydii - C S. sonnei - D
89
Indole negative proteus
Proteus mirabilis
90
OX-2 and 19 source
P. vulgaris
91
Cold enrichment Non motile at 37C
Y. enterocolitica
92
Most common cause of death following transfusion of packed red cells
Y. enterocolitica
93
CPON
1. Staphylococcus 2. Enterobacteriaceae 3. Francisella 4. Oxidizer: Stenotrophomonas, Acinetobacter
94
CPOP
1. Micrococcus 2. Neisseria, Haemophilus 3. Plesiomonas, Aeromonas, Vibrio 4. Oxidizer: Pseudomonas, Burkholderia 5. Campylobacter, Helicobacter, Chromobacterium

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