Microbiology 4 Flashcards
(128 cards)
1
Q
Halophilic Vibrio except
A
V. cholerae and V. mimicus
2
Q
Sucrose fermenters of Vibrio
(colony and medium)
A
V. cholerae and V. alginolyticus
Yellow on TCBS
3
Q
Spread through eating raw/undercooked shellfish
A
Cholera
4
Q
Cholera biotype resistant to polymyxin B
A
El Tor
5
Q
Cholera subgroups associated with pandemic cholera
A
V. cholerae O1 and O139
6
Q
Cholera serogroups
A
Inaba, Ogawa, Hikojima
7
Q
Susceptible to 0/129
A
V. cholerae
8
Q
String test reagent
A
0.5% Sodium deoxycholate
9
Q
“Summer diarrhea”
A
Vibrio parahaemolyticus
10
Q
Lactose positive Vibrio
A
V. vulnificus
11
Q
Pink-centered/bull’s eye colony on CIN
A
Aeromonas
12
Q
Fresh-water fish
A
Aeromonas
13
Q
Plesiomonas colony and medium
A
White to pink in INOSITOL brilliant green bile salt agar
14
Q
Aeromonas identifying test
A
Mannitol positive
15
Q
Plesiomonas identifying test
A
Inositol positive
16
Q
Broth with 6.5% NaCl
A
Vibrio
17
Q
Ammonium cyanide odor on SBA
Violent pigment
CPOP
A
Chromobacterium violaceum
18
Q
Campylobacter culture medium
A
Campy-BAP
Skirrow’s
19
Q
Campylobacter jejuni vs Helicobacter pylori
A
Campylobacter: Cephalotin resistant
Helicobacter: Nalidixic acid resistant
20
Q
Top 3 MDR cause of nosocomial infections
A
- P. aeruginosa
- Acinetobacter baumannii
- Stenotrophomonas maltophilia
21
Q
Asaccharolytic
A
“Hindi sweet si Jason AMOA”
Alcaligenes
Moraxella
Oligella
Acinetobacter iwoffii
22
Q
“Serrated confluent growth” in cetrimide agar
A
PAU
23
Q
PAU is the #1 cause of infection in
A
Burn
Cystic fibrosis
24
Q
“Swimmer’s ear”
Ecthyma gangrenosum
Jacuzzi hot tub/Whirlpool dermatitis
A
PAU
25
One of PAU virulence factor
Exotoxin A
26
Glanders disease
Burkholderia mallei
27
"Vietnamese time bomb" or Meliodosis
Bukholderia (Pseudomonas) pseudomallei
28
Deep pink, wrinkled colonies in Ashdown medium
"Earthy odor"
B. pseudomallei
29
Pink on PC and MAC agar
B. cepacia
30
Lavender green colony on BAP
"Ammonia-like odor"
Yellow pigment on TSA
Stenotrophomonas maltophilia
31
Strong maltose oxidizer
S. maltophilia
32
Blue-grey (Cornflower blue) on EMB
Purple on MAC
Acinetobacter
33
CPON LDC(+) motile
S. maltophilia (vs. Acinetobacter)
34
Alcaligenes vs Bordetella
Urease
(+) vs (-)
35
MAC (-)
DNAse (+)
Flavin pigment
Chryseobacterium meningosepticum
36
HAEMOPHILUS HACEK-before Mycobac
37
Common causes of TB in humans
M. tuberculosis, africanum, cannetti
38
MTB measurement
0.2-0.6 micron in diameter
1-4 micra in length
39
MTB virulence factor that prevents fusion of phagosome and lysosome and recruitment of neutrophils
Cord factor
40
Reliable/rapid method of MTB identification showing parallel chains
Cord formation (Serpentine cords)
41
Droplets of dried sputum in the air for MTB may be infectious for (days)
8-10 days
42
Hours an MTB culture upon sun exposure be killed
2 hours
43
Hours an MTB sputum upon sun exposure be killed
20-30 hrs
44
Dried sputum remains infectious for
6-8 months
45
Droplets of dried sputum remain infectious for
8-10 days
46
Inspissation temp/hrs
75-80C 2hrs 3 days
47
Multinucleated giant cells/fusion of macrophages in MTB
Langhans cell
48
Vit B6 deficiency leading to Peripheral neuropathy
Isoniazid
49
MTB primary drug that cause uric acid crystals
Pyrazinamide
50
MTB primary drug that cause red/green colorblindness
Ethambutol
51
Primary drugs for MTB (TB-DOTS)
"RIPE"
Rifampicin, Isoniazid, Pyrazinamide, Ethambutol
52
Resistant to Rifampin and Isoniazid TB
MDR TB
53
Ideal size DSSM
2-3cm (thumb size)
54
Ideal size smear blood drop
2-3mm
55
Decontaminating and Digesting agents for MTB
NAOH
NALC (N-acetyl-L-cysteine)
56
Diagnose latent TB infection
IGRA Interferon gamma release assay
57
Can detect drug-resistant MTB
GeneXpert (PCR-based NAAT)
58
Swimming pool granuloma
M. marinum
59
Yellow bacillus
M. kansasii
60
Scrofula
Cervical lymphadenitis
Mycobacterium scrofulaceum
61
Tap water bacillus
Mycobacterium gordonae
62
Group II: Scotochromogens
"Sc Sz X gordon"
M. scrofulaceum
M. szulgai
M. xenopi
M. gordonae
63
Group I: Photochromogens
"MASK"
M. marinum
M. asiaticum
M. simiae
M. kansasii
64
M. avium-intracellulare complex SPECIMEN
Stool
65
MTB culture medium for contaminated spx
Petragnani (increased malachite green)
66
Buruli ulcers, "Inert bacillus"
M. ulcerans
67
Inert HACEK
Eikenella corrodens
68
"Radish bacillus"
M. terrae-triviale
69
Group IV: Rapid growers
"FPASCh"
M. fortuitum
M. phlei
M. abscessus
M. smegmatis
M. chelonei
70
Nitrate positive rapid grower
M. fortuitum
71
Mimics TB
"NocHisPa kasi parang may TB"
Nocardia asteroides (bacteria)
Histoplasma capsulatum (fungi)
Paragonimus westermani (parasite)
72
M. leprae stain
Fite-faraco's
(hematoxylin as counterstain)
73
Leprosy manifestation:
Lepromine and CMI (+)
Few AFB
Tuberculoid leprosy
74
Test for M. kansasii
Tween 80 HOH
75
Biochemical tests for MTB and results
Niacin accumulation test - (+) yellow color
Nitrate reduction - (+) broth: pink/red; strip: blue
Pyrazinamidase
MPT64 antigen
T2H susceptibility/inhibition test - (-) inhibition of growth
76
Lumpy jaw, Actinomycosis
Molar tooth colonies
Actinomyces israelii
77
Positive on tap water agar
Nocardia
Streptomyces
Nocardiopsis
78
Positive for the hydrolysis of casein, xanthine, tyrosine
Nocardia transvalensis
79
Negative for the hydrolysis of casein, xanthine, tyrosine
Nocardia asteroides
80
Whipple's disease - PAS-staining macrophages
Tropheryma whipplei
81
Longest bacteria
Borrelia
82
Borrelia culture medium
Barber Stoenner Kelly BSK
33C 6 weeks
83
Soft, painful chancroid
H. ducreyi
84
Hard, painless chancre
Treponema pallidum
85
Tabes dorsalis
Tertiary syphillis - neurosyphilis
Degeneration of lower spinal cord
86
Congenital syphilis triad
Hutchinsonian triad
Notched teeth
Keratitis
Nerve deafness
87
Current treatment for syphillis
Penicillin G
88
Agent of yaws
Treponema pertenue "pertenYAW"
89
Agent of bejel
Endemic syphilis
Treponema endemicum
90
Agent of pinta
Treponema carateum
91
Silver stains
Warthin starry
Levaditi
Fontana tribondeau
92
Severe form of leptospirosis
Weil's disease (icterohemorrhagiae)
93
Gold standard for leptospirosis diagnosis
Microscopic agglutination test
94
Parrot fever
Psittacosis/Ornithosis
Chlamydia psittaci
95
3 causes of Guillain Barre Syndrome
Campylobacter jejuni
Chlamydophila pneumoniae
Zika virus
96
C. pneumoniae culture
Hep2 cells, Human cell lines
97
Associated with TWAR strain
Taiwan Acute Respiratory strain
Chlamydophila pneumoniae
98
Only chlamydia sensitive to sulfonamide
Chlamydia trachomatis
99
Lymphogranuloma venereum
C. trachomatis subtypes L1, L2, L3
100
TRIC
Trachoma, Inclusion conjunctivitis
101
Specimen for NAAT C. trachomatis
Early morning first voided urine
102
Pear-shaped elementary bodies
Chlamydia pneumoniae
103
With glycogen inclusions
Sensitive to sulfonamide
Round elementary bodies
Chlamydia trachomatis
104
All rickettsia cannot survive outside host/vector EXCEPT
Coxiella
105
Human granulocyte erlichiosis
Anaplasma phagocytophila
106
Human monocyte erlichiosis
Ehrlichia chaffeensis
107
Frequent cause of community-acquired pneumonia and tracheobronchitis
Eaton's agent (M. pneumoniae)
108
Selective media for M. pneumoniae
SP4-glucose
109
Genital mycoplasmas
M. hominis
U. urealyticum
Growth on NYCA
110
Medium for M. hominis and U. urealyticum
SP4-arginine
SP4-urea
111
Large fried egg colonies
M. hominis
112
Tiny, fried egg colonies, dark brownish clumps
U. urealyticum
113
Trench fever agent and vector
B. quintana (human body louse)
114
Carrion's disease, Oroya fever, Verruga Peruana fever
Bartonella bacilliformis
115
Cat scratch disease agents
Bartonella henselae
Afipia felis
B. clarridgeaie
116
Alternative for safranin counterstain in gram negative aerobes
0.5% aqueous basic fuchsin
117
Sulfur granules only seen macroscopically
Propionebacterium acnes
118
Cell wall composed of L-diaminopimelic acid
Sulfur granules
Blood culture contaminant
Propionebacterium (Cutibacterium) acnes
119
Anaerobic diphtheroid
Catalase and spot indole positive
Propionebacterium (Cutibacterium) acnes
120
Anaerobic diphtheroid
bifurcated cells "dog bones"
Bifidobacterium
121
Capsule, catalase positive
Black on BBE (20% bile)
Bacteroides fragilis
*BBE - Bacteroides Bile Esculin agar
122
Resistant to Kanamycin, Vancomycin, Colistin
Bacteroides fragilis
123
brick red fluorescence on UVL
Porphyromonas asaccharolytica
Prevotella melaninogenica
124
Do not grow on KVLB
Asaccharolytic
Vancomycin susceptible
Porphyromonas
125
Brown-black pigment on KVLB agar
Colistin susceptible
Prevotella melaninogenica
126
"Bread crumb colonies"
Gram negative bacilli:
Streptobacillus moniliformis (aerobic)
Fusobacterium nucleatum (anaerobic)
127
"bread crumb" colonies
greening on air exposure
chartreuse fluorescence
Fusobacterium nucleatum
128
