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Microbiology Flashcards

(74 cards)

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1
Q

Gram Staining Sequence

A

Methyl Violet(Cystal Violet/Gentian Violet)
Grams Iodine(Mordant-Fixer)
Acetone(Decolonizer)
Safranin(Counterstain)

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2
Q

All Cocci are Gram Positive, except

A

Meningococci
Gonococci
Veilonella
Moraxella

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3
Q

All Bacilli are Gram Negative, except?

A 
Mycobacteria
Anthracis Bacillus 
Clostridium Species 
Diphtheriae Coryne Bacterium
Nocardia Species 
Acitomyces
Listeria 
Diphtheroids
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4
Q

ZN Staining
Other name?
Organisms?

A

Ziehl Neelson Staining/Acid Fast Staining

MTB 20%
M. Leprae 5%
Nocardia 1%

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5
Q

Ability to resist decolourisation comes because of____________content

A

Mycolic Acid Content

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6
Q

Sequence of ZN staining for MTB

A

For MTB Sputum Smear:

Sputum Smear 
Carbol Fuchsin
Intermittent Heating
20% H2SO4(Decolourizer)
Methylene Blue(Counter Stain)
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7
Q

Bipolar Staining Organisms

A 
Yersinia pestis(Plague)
Haemophilus ducreyi(Genital Ulcer)
Calymatobacter(Klebsiella)
Burkholderia Mallei(Glanders Disease-Horse Disease(motile))
Burkholderia Psuedo Mallei(motile)
Vibrio Parahemolytics(Sea food poisoning )
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8
Q

Cuneiform Arrangement is seen in________?

A

Albert Staining of Cornyebacterium Diphtheriae

Stained Green
AKA Chinese Letter Pattern

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9
Q

Capsulated Organisms

A 
Pneumococcus/Pseudomonas
Antarcis Bacillus 
Klebsiella
Influenza Haemophilus
Yersinia Species 
Bordetella Species 
Meningococci
Clostridium Perferingens(Welchii)
Vibrio Parahemolyticus
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10
Q

Most capsules are made of___________?

A

Polysaccharides

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11
Q

Capsules of Bacillus Anthracis is made of________?

A

Polypeptide

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12
Q

Quellung Reaction is seen in __________?

A

Pneumococcus

Capsular Antigen + Capsular Antibody = Capsular Swelling

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13
Q

MacFadyean’s Reaction is seen in ___________?

A

Bacillus Anthracis

Polypeptide capsule stained by Methylene Blue

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14
Q

Function of Capsule?

A

Antiphagocytic

Prevent Opsonisation

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15
Q

Opsonisers are?

Which is the major Opsoniser?

A
  1. C3b - (Major Opsoniser)

2. Fc of IgG and IgM

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16
Q

Function of Biofilm?

A
  1. Adherence to the surface
  2. Antibiotic Resistance

(Biofilm is modification of the capsule)

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17
Q

Tumbling Motility is seen in________?

A

Listeria

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18
Q

Darting motility is seen in____________?

A

Vibrio Cholera

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19
Q

Swarming Motility is seen in ____________?

A

Proteus

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20
Q

Falling Leaf like motility is seen in________?

A

Giardia Lamblia

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21
Q

Slow form of Swarming is called ________?

Its seen in ____________?

A

Stately Motility

Different species of Clostridium Species

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22
Q

Spirochetes are?

A

Endoflagella

arise from periplasmic space

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23
Q

Methods to demonstrate motility?

A
  1. Hanging Drop
  2. Semisolid Agar Method
  3. Phase Contrast Microscopy
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24
Q

Semi Solid Agar contains _______% agar

A

0.5-1% Agar

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25
Nutrient Agar contains _____% agar
2% Agar Contains peptone meat extract
26
Nutrient Broth contains _____% agar?
Zero % Agar
27
Enriched Media are?
1. Blood Agar 2. Chocolate Agar 3. Loffler Serum Slope
28
Enriched media are solid/liquid?
Enriched - Solid
29
Blood Agar contains _______% blood?
5% Sheep Blood
30
Loffler Serum Slope is used for _______?
C. Diphtheriae
31
Enrichment Media is Liquid/Solid?
Enrichment Media is always Liquid
32
Enrichment media for Salmonella?
Selenite F Broth
33
Enrichment media for Shigella?
Selenite F Broth
34
Selective media is mostly liquid/solid?
Selective media is mostly solid?
35
TCBS is ___________in colour? TCBS is ___________ in colour when V. Cholera is used?
TCBS is Green in colour. Turns to Yellow when V. Cholera is present.
36
Selective Media for V. Cholera?
``` TCBS Thiosulphate Citrate Bile Salt Sucrose ```
37
___________is the selective media for MTB?
Lowenstein Jensen(LJ) is the selective media for MTB
38
_________ is the selective Agent for MTB in LJ Media?
Malachite Green is the selective Agent for MTB in LJ Media
39
In MacConkey Agar, lactose fermenters show ___________ colour change?
In MacConkey Agar, lactose fermenters show PINK colour change
40
In MacConkey Agar, non-lactose fermenters show ___________ colour change?
In MacConkey Agar, non-lactose fermenters show no colour change
41
MacConkey Agar is a selective media for __________?
Only Gram Negative Bacteria will grow on MacConkey Agar
42
Contents of MacConkey Agar?
``` Peptone Lactose Agar Neutral Red Taurio Chocalate Sodium ```
43
Lactose Fermenters are?
E coli Klebsiella Shigella Sonnei
44
In Robertson's Cooked Meat Media, when the meat pieces turn black, what is the reaction called?
When meat pieces turn black, it is a Proteolytic Reaction
45
In Robertson's Cooked Meat Media, when the meat pieces turn black, which is the organism present?
Clostridium tetani | Colour change due to Proteolytic reaction
46
In Robertson's Cooked Meat Media, when the meat pieces turn pink, which is the organism present?
Perferingens welchii | Sacchrolytic Reaction
47
Transport media of V.Cholarae? Which other Media can also be used?
V-R Media Venkat Raman Ramakrishnan Media Cary Blair Media can also be used
48
Transport media of Streptococcus pyogenes?
Pikels
49
Transport media of Gonococeif meningococci?
Stuarts and Amies
50
Transport media of Shigella(salmonella)?
Sach's Buffered Glycerol Saline
51
Obligate Anaerobes are?
Acitinomycetes Bacteroids Clostridium
52
Facilitated Anaerobes are?
Staphylococcus | Streptococcus
53
Capnophile organisms are?
Brucella abortus | Haemophilus influenza
54
Micro aerophiles are?
Compylobacter | Helicobacter
55
Organisms requiring -10 to +10°C is known as___________?
Psychrophile
56
Organisms requiring 20 to 40°C is known as___________?
Mesophile
57
Organisms requiring more than 45°C is known as___________?
Thermophile
58
Organisms requiring more than 100°C is known as___________?
Thermus aquaticus
59
Father of PCR?
Kary B Mullis
60
____________ is Catalase Positive?
Staphylococcus is Catalase Positive
61
____________ is Catalase Negative?
Streptococcus is Catalase Negative
62
Coagulase Test Positive indicates __________? | Eg.____________
Pathogenic Staphylococcus is Coagulase Positive. | Staph. aureus
63
Coagulase test Negative indicates______________? | Eg.___________
Non pathogenic Staphylococcus. Eg. Staph. Epidermis Staph. Saprophyticus
64
Staph. Epidermidis is Novobaicin ______?
Staph. Epidermidis is Novobaicin Sensitive
65
Staph. Saprophyticus is Novobaicin_________?
Staph. Saprophyticus is Novobaicin Insensitive/Resistant
66
Antibiotic Sensitivity Testing is conducted on _____________ agar?
AST is conducted on Mueller Hinton Agar(MHA) (Nutrient Agar) AST aka Kirby Baur Disc Diffusion Testing
67
Antibiotic Sensitivity Testing is also known as?
Kirby Baur Disc Diffusion Testing
68
Young women develops UTI. | MCC is?
MCC for female UTI is E.coli
69
Female UTI | MC GPC is?
S.Saprophyticus
70
Violet coloured grape like clusters are seen in __________ ?
Staph. aureus
71
Staph. aureus in Nutrient Agar is seen as?
Golden Yellow Pigmentation
72
Staph. aureus in Blood Agar is seen as?
B hemolysis | Pin head size colonies
73
Best selective media for Staph. aureus?
Mannitol Salt Agar is the best Ludham's Media can also be used.
74
Binding of Protein A (present in COWAN-1 strain of Staph. aureus) with FC portion of IgG is known as?
Co-agglution Test

CJJ (6 decks)

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