Microbiota and Mucosal Barriers - Flashcards Preview

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Flashcards in Microbiota and Mucosal Barriers - Deck (19):
1

What does the mucus blanket do in the intestines?

 

What produces them?

 

What do they contain?

Forms a selective barrier 

 

Goblet cells

 

mucins, ions, antibodies, antimicrobial peptides, and bacteria

2

How do commensal organisms co-exist with the mucus blanket?

  • specific binding via adhesins
  • grazing on sugars from mucus oligosaccharides
  • grazing on mucins

3

How does bacterial infection change expression of the mucus blanket?

 

 

LPS and bacteria cause upregulation of the MUC gene, increasing mucin expression

 

 

 

4

What are some defenses pathogens have developed to circumvent mucus?

  • Flagella/motility proteins
  • Secretion of mucus-digesting enzymes
  • Disturbance of tight junctions
  • Toxins that reduce mucus production

5

Other than a physical barrier, how does mucus protect against bacteria?

  • Secretion of IgA
  • Acidification of the Environment
  • Oxygen Radical Production
  • Defensins
  • Lysozymes/Acid hydrolases (digest bacteria)
  • Lactoferrin (competes for nutrients)

6

What are five anti-microbial proteins produced by the Paneth cells? 

 

What do they do?

  • a-defensins: form pores in cell membranes to disrupt their integrity
  • Lysozyme C: hydrolyzes bacteria cell walls
  • Phospholipase A2: hydrolyzes bacterial cell membranes
  • RegIIIy- Binds to pepetidoglycan of Gram + (induced by TLR activation)
  • Cryptdin related sequence: anti-microbial

7

How do pathogens evade anti-microbial peptides?

  • Capsule formation
  • Surface charge modification
  • Protease secretion
  • Efflux pumps
  • Modultate AMP expression

8

Which antibodies are secreted?

 

 

How is mucosal IgA different from serum IgA?

IgA, IgM, IgG, IgE, and IgD

 

 

Serum IgA is mostly monomeric, while mucosal IgA is mostly polymeric

9

By what mechanisms does IgA work against pathogens?

 

 

How have pathogens evolved against IgA?

  • Inhibit adherence to mucosa
  • Trap within mucins
  • Neutralize viruses
  • Neutralize enzymes/toxins

 

  • IgA proteases
  • Glycosidases
  • IgA binding proteins 

10

How are bacteria in the gut classified?

 

How does microbial composition of the microbiome affect disease states? 

Symbionts, commensals, and pathobionts

 

Many inflammatory disease states are associated with overgrowth of pathobionts or undergrowth of symbionts/commensals.  (ASSOCIATED, not necessarily caused by)

11

How does the microbiota change, moving distally through the gut?

 

How is the microbiota composition determined?

Becomes more diverse with more bacteria overall

 

 

Oxygen availability, antibiotic use, diet, environment, immune regulation

12

What are the four main anaerobic genera seen in the intestinal microbiota?

 

What are the four main aerobic genera?

Anaerobic

  • Bifidobacterium
  • Clostridium
  • Bacteroides
  • Eubacterium

Aerobic 

  • Escherichia
  • Enterococcus
  • Streptococcus
  • Klebsiella

13

How does absence of a microbiota affect immune development?

Microbiota allows the immune response to develop

  • lymphocyte infiltration
  • increased antibody diversity
  • increased B cell response
  • decreased mature lymphatic tissue

14

How do SCFA regulate the immune response?

  • commensal bacterias produce SCFA 
  • SCFAs regulate PMNs, dendritic cells, and macrophages
  • if pathogens expand, killing commensals, SCFA decreases, activating immune system

15

How does the microbiota protect against C. difficile?

Competes for nutrients and space; C diff typically only occurs following antibiotic use

16

How does the microbiota help to regulate digestion?

  • Mediates bile acid synthesis
  • lipid absorption
  • amino acid metabolism
  • vitamin synthesis
  • SCFA production

17

What diseases/conditions might be caused by microbiota imbalances?

  • Inflammatory Bowel Disease
  • Obesity
  • Diabetes
  • Crohn's Disease
  • Cancer
  • Allergies/Asthma

18

What bacterial shift is seen in inflammatory bowel disease patients?

Shift from obligate anaerobes to facultative anerobes

(specifically from bacteroides to proteobacteria)

19