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Histo LAB > Microscope > Flashcards

Microscope Flashcards

1
Q

Magnify image and allow visualization of greater details than is possible with the unaided eye

A

LIGHT MICROSCOPY

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2
Q

2 CLASSIFICATIONS OF LIGHT MICROSCOPY

A

Simple
Compound

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3
Q

One lens
Simplest – magnifying glass or pair of reading glasses

A

Simple Microscope

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3
Q

Multiple lens

A

Compound Microscope

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4
Q

– the power of the microscope to enlarge the image of an object

A

Magnification

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5
Q

– ability of the microscope to distinguish two objects from each other

A

Resolution

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6
Q

PARTS OF MICROSCOPE
Has 2 major parts:

A

Optical parts
Mechanical parts

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7
Q

– magnifies tissue specimens

A

Optical parts

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8
Q

– supports the optical parts

A

Mechanical parts

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9
Q

OPTICAL PARTS

A

Ocular lens / Eyepiece
Objective lens

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10
Q

lens that one looks into
Magnifies 10x

A

Ocular lens / Eyepiece

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11
Q

– shortest, red

A

Scanner (4x)

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12
Q
  • yellow
A

Low power (10x)

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13
Q
  • blue
A

High power (40x)

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14
Q
  • longest, b&w
A

Oil Immersion (100x)

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15
Q

MAGNIFICATION:
Multiply the __________ by the______

A

eyepiece magnification (10x) by the objective magnification used (4x, 10x, 40x)

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16
Q

Connects ocular tube and base. It also helps carry the microscope

A

Arm (limb)

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17
Q

Supports the lenses and nosepiece of the microscope and connects to the base of the microscope

A

Arm

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18
Q

Provides support to help microscope stand upright

A

Base

19
Q

Focus of image under high power and moving the stage up and down.

A

Coarse adjustment knobs

20
Q

Lower or raise the stage

Used for focus on scanning. Usually, the low power lens is used, enabling the movement of the tube.

A

Coarse adjustment knobs

21
Q

Forming a cone of all the dispersed light rays from the illuminator

A

Condenser

22
Q

Gathers light from the microscope’s light source and concentrate it into a cone of light that illuminates the specimen

A

Condenser

23
Q

Controls the intensity of illuminating light

A

Diaphragm (Iris)

24
Q

Helps in controlling the amount of light that is passing through the opening of the stage

A

Iris diaphragm

25
Q

Magnification of image produced by objective lens

A

Eyepiece (ocular lens)

26
Q

Focus the image when viewing under high power

A

Fine adjustment knobs

27
Q

Used for focusing on high power and oil objectives.

Moves the body tube for focusing when using the high power lens.

A

Fine adjustment knobs

28
Q

Provide high-intensity light at the field aperture

A

Illuminator

29
Q

Reflects light from an external source

A

Mirror

30
Q

Primary magnifier of microscope

A

Objective lens

31
Q

Maintains the correct distance between the ocular and objective lens

A

Ocular tube

32
Q

Holds the objective lens.
Its rotation helps to change the power of the objective lens

A

Revolving nose piece

33
Q

Holds the different objective lenses
It allows the rotation of the lenses while viewing

A

Revolving Nosepiece

34
Q

Place for holding sample

A

Stage

35
Q

Platform that is flat; used for placing the slides under observation

A

Stage and stage clips

36
Q

Keeps the slide with a specimen in place on the stage

A

Stage clips

37
Q

move the stage sideways

A

Stage controls

38
Q

Scanner:
Eyepiece Magnification
Objective Magnification
Overall Magnification

A

x10
x4
40

39
Q

Low Power:
Eyepiece Magnification
Objective Magnification
Overall Magnification

A

x10
10
100

40
Q

High Power:
Eyepiece Magnification
Objective Magnification
Overall Magnification

A

x10
x40
400

41
Q

Oil Immersion:
Eyepiece Magnification
Objective Magnification
Overall Magnification

A

x10
x100
1000

42
Q

FOCUSING SPECIMENS

A
  • Always start with the Scanning Objective
  • Place your specimen in the center of your field of view
  • Use the Coarse Knob to focus.
  • Next, use the Fine knob until clear
  • Once you’ve focused on scanning, switch to Low Power objective using the revolving nosepiece.
  • Repeat steps 3 & 4.
  • Now, switch to High Power.
  • At this point, ONLY use the FINE ADJUSTMENT KNOB to focus specimens.
43
Q

Scanning
Low Power

A

use COARSE and FINE knobs

44
Q

High Power
Oil immersion

A

use FINE knobs

Histo LAB (13 decks)

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