Microscope Flashcards
What kind of microscope do we use at Seneca?
Compounding light microscope
How do compounding light microscopes work?
Generate image by using a combination to increase magnification.
What is the optical tube?
The optical tube is the distance between the objective lens and eyepeice. Typically 160mm in length.
Can the optical tube be adjusted?
Typically not.
What is the eyepiece?
Where you view the slide. Can be binocular (two eyepieces) and monocular (single eyepiece)
What is the stage?
Holds the slide under objective lens, and moves it back and forth and side to side when you move the controls.
Allows light to pass through slide for viewing.
What is coarse focus?
Larger knob. Moves stage up and down quickly. Allows you to focus image.
What is fine focus?
Smaller knob. Moves stage up and down as well but much slower. Allows you to focus image.
What is the condensor?
Two lenses that focus light from the illuminator on the slide.
Can be adjusted to change intensity of the light source.
What is the diaphragm?
Opens and closes to control the amount of light illuminating the object.
What is the magnification of the ocular lens?
x10 magnification
What is the advantage of a binocular lens verses a monocular lens?
Binocular gives a better 3-D image.
How many objective lenses do most microscopes have?
3-4
What is the magnification of the 4 lenses?
x4
x10
x40
x100 (oil immersion)
What is the purpose of the oil immersion lens?
Cytologies, blood smears
