Microscope (block one)

Question 1

objectives

Answer 1

scanning: 4x * 10x = 40x
lower power 10x * 10X = 100x
high power: 40x * 10x = 400x
oil immersion: 100x * 10x = 1000x

Question 2

diopter adjustment

Answer 2

correct for difference between eyes

Question 3

compound light microscope

Answer 3

two lenses: objective and occular
disecting and compounding

Question 4

illumination

Answer 4

light source

Question 5

condenser

Answer 5

gathers and focuses light

Question 6

iris diaphragm

Answer 6

adjusts the amount of light
(right below the stage)

Question 7

aperture

Answer 7

hole in the middle of stage that lets light through

Question 8

the graticule

Answer 8

more suitable ‘ruler’ for measuring cells

Question 9

what size is a typical slide graticule

Answer 9

1mm

Question 10

1 division at 4x

Answer 10

25 um

Question 11

1 division at 10x

Answer 11

10 um

Question 12

1 division at 40x

Answer 12

2.5 um

Question 13

1 division at 100x

Answer 13

1.0um

Question 14

who invented the first microscope

Answer 14

Janssen’s
magnification 3x-9x

Question 15

Robert Hooke

Answer 15

Micrographia (book) –> first depcition of an microorganisms, micro-fungus (Mucor)

Question 16

existence of microscopic organisms was discovered by

Answer 16

Robert Hooke
Anton van Leeuwenhoek

Question 17

Anton van Leeuwenhoek

Answer 17

leeuwenhoek observed and described microscopic protozoa and bacteria

Question 18

Transmission electron microscope (TEM)

Answer 18

tomography
internal ultrastructural features such as nucleus
ultra-thin specimens

Question 18

Laser scanning confocal microscope

Answer 18

3 lasers –> red, blue, green
fresh wet preparations

Question 19

Scanning Electron Microscopy (SEM)

Answer 19

surface of object
topography
whole specimens coated with inert metal (gold)

Question 20

what is the backbone of understanding microbes in infectious diseases

Answer 20

microscopes