Microscopes Flashcards
(21 cards)
micrograph
A photo taken through a microscope
Scanning electron microscope
provide information about the surface features of a specimen, the SCM operates up to a magnification of 300,000 X produce dimensional images of cells
Electron microscopes
Use a beam of electrons instead of light and only thin and dead cells can be observed
contrast
it may be difficult to see cell parts because the cell on its background may be paler, transparent, so stains are used to improve the contrast between a cell structure and the background to produce better images
Common stains are methylene blue and iodine
Resolution
being able to see clear, detail in an image depends on the resolution or resolving power of the microscope
Resolution is the ability to distinguish between two objects that are very close together
What do microscopes do and why are they important?
Allow us to see very small things, and it is useful for our health to look for abnormalities
Compound light microscope
The compound light microscope uses two sets of lenses to magnify the object
Illumination is provided by light source on the base of the microscope, and the total magnification can be found by multiplying the power of the objective lens by the ocular lens
Tube
Separates the ocular lens from the objective lens
Revolving nose piece
Hold the objective lenses
Objective lenses
Magnify specimen, three lenses are usually 4X 10 X and 40 X
stage
Supports the slide
Diaphragm
allows like to pass through the specimen
condenser lens
Focusses light onto the specimen
Lamp
Supplies the light that passes through the specimen
Base
Provide a stable platform for the microscope
fine adjustment knob
sharpens an image
Course adjustment knob
Move the stage up or down to focus the specimen
Stage clips
Hold the slide in position on the stage
Arm
holds the tube in place and is used to carry the microscope
Eyepiece or ocular lens
magnify the specimen usually by 10 X, single lens
