microscopes (light & electron)

Question 1

What does the light microscope use to magnify specimens?

Answer 1

Light and glass lenses.

Question 2

What are the 3 objective lenses in a light microscope?

Answer 2

LPO (4x), MPO (10x), HPO (40x).

Question 3

What is the total magnification using the HPO?

Answer 3

400x (10x eyepiece × 40x objective).

Question 4

Define resolution in microscopy.

Answer 4

The ability to distinguish detail or separate two close objects.

Question 5

Why is the letter ‘e’ upside-down under a microscope?

Answer 5

The image is flipped due to the way lenses bend light.

Question 6

What does an electron microscope use instead of light and lenses?

Answer 6

Electron beams and electromagnets.

Question 7

What type of image does a SEM produce?

Answer 7

A 3D image of the surface.

Question 8

What type of image does a TEM produce?

Answer 8

A 2D image of internal structures.

Question 9

Name one advantage of a light microscope.

Answer 9

Cheaper, easier to use, can view live specimens.

Question 10

Name one disadvantage of an electron microscope.

Answer 10

Expensive, cannot view live specimens.