Microscopy

Question 1

is a laboratory equipment used to view complex, minute, structures; invented by Anton van Leeuwenhoek whose first specimen was pond water.

Answer 1

Compound light microscope

Question 2

a microscopic animal

Answer 2

Animalcules

Question 3

Three major parts of microscope:

Answer 3

Optical, Illuminating, Mechanical.

Question 4

are used to view specimens, illuminating parts are those involved with light provision and adjustment.

Answer 4

Optical parts of the microscope

Question 5

Compound light microscope considers three parameters:

Answer 5

Magnification, Resolution, Contrast

Question 6

extends our ability to observe the details 1000 times, so that we can see objects as small as 0.1 micrometer in diameter

Answer 6

Magnification

Question 7

resolving power is the
ability to distinguish two adjacent
objects as distinct and separate.

Answer 7

Resolution

Question 8

typically improves the final image, as this will accentuate differences in parts of the specimen

Answer 8

Contrast

Question 9

Is also known as “ocular”, the part used to look through the microscope. It have a standard magnification of 10x

Answer 9

Eyepiece/Ocular Lense

Question 10

Moves the stage up and down to bring the specimen into focus

Answer 10

Coarse Adjustment

Question 11

Brings the specimen
into sharp focus under low power

Answer 11

Fine Adjustment

Question 12

Provides the lowest magnification power in all of the objective lenses (4x)

Answer 12

Scanning objective Lens

Question 13

The part connecting the base and to the head and the eyepiece to the base of the microscope

Answer 13

Arm

Question 14

Where the microscope arm connects the microscope base

Answer 14

Inclination joint

Question 15

It carries the microscopic illuminators and acts as microscope support.

Answer 15

Body tube

Question 16

Also known as the “revolving turret”. It holds the objective lenses and is moveable

Answer 16

Nosepiece

Question 17

also called high dry lens, is ideal in observing fine details within the specimen sample.

Answer 17

High power objective (HPO)

Question 18

It has more magnification power than the scanning lens, and is one of the most helpful when it comes to observing

Answer 18

Low power objective (LPO)

Question 19

holds the slide in place, In a microscope with a mechanical stage

Answer 19

Clips

Question 20

holds the slides supported by stage clips

Answer 20

Stage

Question 21

It is also known as the Iris. It is located under the stage of the microscope

Answer 21

Diaphragm

Question 22

is used to reflect light from an external source up through the stage’s bottom.

Answer 22

Mirror

Question 23

It is the bottom part that serves as a support for the microscope.

Answer 23

Base

Question 24

Mechanical

Answer 24

Coarse adjustment
Fine adjustment
Inclination joint
Arm
Body tube
Nose piece
Clips
Stage
Base

Question 25

Optical

Answer 25

Eyepiece Scanning objective Lens High power objective (HPO) Low power objective (LPO)

Question 26

Illuminating

Answer 26

Mirror

Question 27

This is the ability of a microscope to stay relatively in focus as the user switches among the objectives

Answer 27

parfocal

Question 28

is when the object or specimen being viewed is magnified to its maximum limit.

Answer 28

total magnification

Question 29

refers to the finest detail that a microscope can resolve when imaging a specimen;

Answer 29

resolving power

Question 30

is the maximum area visible through the lenses of a microscope, and it is represented by a diameter.

Answer 30

field of vision

Question 31

LPF Conversion

Answer 31

LPF=1mm=1000um

Question 32

HPF Conversion

Answer 32

HPF=LPO/HPOxLPF

Question 33

Calculating Specimen Size (length of cell)

Answer 33

length of cell(um)= Field of vision/number of cells