Microscopy

Question 1

What are the two types of microscopes?

Answer 1

Electron

Light

Question 2

What are the two types of electron microscopes?

Answer 2

Scanning

Transmission

Question 3

What do Transmission Electron Microscopes show?

Answer 3

A 2D image of the inside of the specimen

Question 4

What does a Scanning Electron Microscope show?

Answer 4

A 3D image of the outside of the specimen

Question 5

What is another name for the eyepiece lens?

Answer 5

Ocular lens

Question 6

What is the average magnification for the ocular lens?

Answer 6

10x

Question 7

What is the other lens called?

Answer 7

The objective lens

Question 8

What is the smallest magnification of an objective lens?

Answer 8

4x

Question 9

How do you work out the total magnification?

Answer 9

Multiply the magnification of both the ocular and objective lenses together

Question 10

What is magnification?

Answer 10

How much bigger a specimen is through a microscope

Question 11

How do you calculate the actual size of a specimen once it has been magnified?

Answer 11

Length of specimen times the length on the scale line

All upon the length of the scale line

Question 12

What is important to remember when calculating the actual size of a specimen?

Answer 12

Keep your unit of measurement (for the scale line and the specimen) the same

Question 13

What is cell theory?

Answer 13

All living organisms consist of cells
The cell is the basic and smallest unit of life
All cells originate from existing cells

Question 14

What are the fourteen parts of a microscope?

Answer 14

Eyepiece lens
Coarse adjustment knob
Fine adjustment knob
Arm
Hinge
Base
Light source
Iris diaphragm
Condenser 
Stage
Clips/clamps
Objective lenses 
Nosepiece 
Microscope tube

Question 15

What is the function of the eyepiece lens?

Answer 15

Lens closest to the eye

Magnifies the image

Question 16

What is the function of the coarse adjustment knob?

Answer 16

Moves the stage/microscope
Large movements
Brings the image into focus

Question 17

What is the function of the fine adjustment knob?

Answer 17

Moves the stage/microscope
Precise movements
Clearer focus

Question 18

What is the function of the arm?

Answer 18

Handle used to carry the microscope

Connects the stage, microscope tube and adjustment knobs to each other

Question 19

What is the function of the hinge?

Answer 19

Allows the angle of the arm to be changed

Question 20

What is the function of the base?

Answer 20

Bottom of the microscope

Stability

Question 21

What is the function of the light source?

Answer 21

Attached to the base

Passes light up through a hole in the stage, through the specimen and up to the eye

Question 22

What is the function of the iris diaphragm?

Answer 22

Opening in the condensor

Regulates the amount of light allowed to pass through the specimen

Question 23

What is the function of the condensor?

Answer 23

Concentrates light rays from the light source onto the specimen

Question 24

What is the function of the stage?

Answer 24

Flat surface
Has an opening to allows light through it
The specimen slide is placed on this surface

Question 25

What is the function of the clips/clamps?

Answer 25

Holds the specimen in place

Question 26

What is the function of the objective lenses?

Answer 26

Magnify the images Usually three Have different magnifications

Question 27

What is the function of the nosepiece?

Answer 27

Holds the objectives Rotates Allows the observer to choose which objective lens to use

Question 28

What is the function of the microscope tube?

Answer 28

Holds the eyepiece in place | Connects it to the objective lenses

Question 29

How is a specimen usually studied?

Answer 29

On a microscope slide in a mounting medium

Question 30

What is a microscope slide?

Answer 30

A rectangular glass plate on which the specimen is placed for study

Question 31

What is a mounting medium?

Answer 31

The liquid in which the specimen is placed

Question 32

What is the function of the mounting medium?

Answer 32

It can sometimes be used to stain the specimen to see it better

Question 33

What is a cover slip?

Answer 33

A thin square glass plate that is placed on top of the specimen

Question 34

What is the function of the cover slip?

Answer 34

Prevents the objective lens from coming into contact with the mounting medium Keeps the specimen in position Reduces light refraction

Question 35

What is a wet mount?

Answer 35

A specimen that has been placed on a slide Placed in a mounting medium Covered with a cover slip

Question 36

How do you prepare a wet mount?

Answer 36

Place the cover slip at angle against the drop of mounting medium Keep it in place with a dissecting needle Lower the cover slip carefully to make sure that there are no air bubbles Carefully wipe off any excess liquid

Question 37

What is a micrograph?

Answer 37

A photograph or drawing of a specimen as viewed through a microscope

Question 38

What happens when the magnification of an image increases?

Answer 38

The image becomes larger and more detailed