Microscopy

Question 1

Platform where the slide is positioned with the clips to hold it in place

Answer 1

Stage

Question 2

Reflects light up through the slide to the eyepiece

Answer 2

Mirror

Question 3

Concentrates the rays of light and directs them into object being used

Answer 3

Condensor

Question 4

The lens the the operator looks through

Answer 4

Ocular Lens

Question 5

Tube that the eyepiece and nosepiece are attached to

Answer 5

Body Tube

Question 6

Moves the body tube up and down for focusing (coarse and fine)

Answer 6

Adjustment knobs

Question 7

Lenses used to see the detail of the specimen (scanning, low power or high power)

Answer 7

Objectives

Question 8

Can be adjusted like a camera shutter to control light passing through the object and the body tube

Answer 8

Diaphragm

Question 9

Supports and stabilises the microscope

Answer 9

Base

Question 10

Holds the body tube and can be used as a handle for holding the microscope when carrying

Answer 10

Arms

Question 11

The magnification on the ocular lens is

Answer 11

10x

Question 12

Magnification on Scanning Objective is __
Magnification on Low Power Objective is __
Magnification on High Power Objective is __

Answer 12

4x
10x
40x

Question 13

What is the total magnification for each lens? (Multiply the ocular lens by the objective lens)
Scanning __
Low Power __
High Power __

Answer 13

40x
100x
400x

Question 14

How do you make the specimen appear the brightest and the darkest?

Answer 14

Brightest: Turn Anti-clockwise
Darkest: Turn Clockwise

Question 15

Why is it important to consider depth of field when looking at a tissue specimen?

Answer 15

As you increase magnification the depth of field is reduced, which is why you have to be mindful of specimen placement

Question 16

What measurements are used to measure magnification?

Answer 16

Metres: m
Centimetres: cm 
Millimetres: mm
Micrometres: um
Nanometres: nm

Question 17

1 metre=
1 cm=
1 mm=
1 um=

Answer 17

100 cm
10 mm
1000 um
1000 nm