Microtomy Flashcards by Yasmin Ali

Type of microtome that is a refrigerated apparatus used in fresh tissue microtomy

Cryostat Microtome

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Type of microtome mainly used for cutting celloidin embedded block

Sliding microtome

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Type of microtome used for situations where rapid analysis of tissues is required

Cryostat microtome

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Parts of Rotary microtome:

Part of the knife holder that adjust the knife angle

Knife tilt

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Mechanical device for cutting thin uniform slices of tissue sections

Microtome

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What is the thickness range of the rocking microtome?

2mm to 24mm

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Most commonly used microtome used to cut thin sections of samples visible only with the aid of a light microscope

Rotary microtome

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Parts of Rotary microtome:

A platform which has rails that secure the knife holder base

Microtome base plate or stage

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Parts of Rotary microtome:

A part that anchors the knife holder to the microtome stage

Knife holder base

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Part of rotary microtome used for section thickness

Micron adjustment

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Parts of Rotary microtome:

Part of the knife holder that holds the blade

Blade clamp

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Type of microtome that is used for cutting thin sections of wax embedded material of either animal or plant origin

Rocking microtome

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Who invented the rotary microtome?

Minot

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Cryostat microtome is used to maintain low ______ temperatures.

Cryogenic

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Knives used in ultrathin microtome are usually made from _____, ______ or ________.

Glass
Diamond
Sapphire

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Part of rotary microtome that turns in one direction and advances the block toward the knife at the specified microns

Advancement handwheel

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Part of rotary microtome that holds the paraffin block in place

Casette clamp or block holder

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Type of microtome designed to prepare very thin slices of material for study under a microscope

Ultrathin microtome

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Part of sliding microtome that quickly raises or lowers the specimen 450 um per turn

Coarse advance/Trim control

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Parts of Rotary microtome:

Part of the knife holder that guides the ribbons away fron the blade and towards the operator

Face plate

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Part of sliding microtome that secure the guards in the chosen position

Black knobs

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Means cold

“Cryo”

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Type of microtome used for cutting thin to semi-thin sections of fresh. frozen tissue.

Freezing microtome

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Part of sliding microtome that is moved to the central position to cover the working area of the knife

Knife guard

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Rocking microtome uses what type of razor knives?

A-type biconcave razor knives

Type of microtome used in paraffin embedding block

Sliding microtome

Sliding microtome's knife is mode ______ against a fixed block

Horizontally

Process by w/c processed tissue most commonly a paraffin embedded tissue, is trimmed and cut into uniformly thin slices or sections

Microtomy

Part of rotary microtome that automatically regulates the thickness of the section

Ratchet feed wheel

Cryostat microtome is maintained at a temperature between _____.

-5 to -30 degrees celsius (ave -20 degrees celsius)

Type of microtome that is recommended for elementary biology and pathology students

Rocking microtome

Who invented the rocking (cambridge) microtome?

Paldwell Trefall

Part of rotary microtome that moves the block holder either toward the knife or away from the knife

Coarse handwheel

Give four instances where the freezing microtome is especially needed.

Rapid diagnosis Histological demo of fat Neurological structures Sensitive tissue constituents that are damaged or destroyed by heat

what is the lenght of plane-wedge knife?

100 mm

The knife of the rotary microtome is placed in a ______ position and is therefore dangerous.

blade-up

Part of rotary microtome which causes reciprocal motion of the knife over the block when operated

Flywheel

Basic instrument used in microtomy

Microtome

Used to streched the sections under the temp. of 5 - 10 degrees celsius

Flotation Water bath

Type of sliding microtome that is favored in lab where very hard tissue or large blocks are usually sectioned

Based-Sledge microtome

Type of sliding microtome suited for sectioning specimens embedded in all forms of media

Base-sledge microtome

Rotary microtome: The knife and block holder are brought together by ______ and ______ motion

Upward, vertical

Type of sliding microtome that are ideal for resin-embedded decalcified bone

Base-sledge microtome

Type of sliding microtome wherein the block remains stationary while the knife is moved backward and forward during the process of. sectioning

Standard sliding microtome

Give 3 uses of cryostat microtome in preparing thin sections of fresh tissues

Fluorescent Ab staining technique Histochemical enzyme studies Rapid preparation of urgent tissue biopsies for intraoperative diagnosis

the ultrathin microscope is primarily used for cutting sections at _____ micra, for electron microscopy.

0.5 micra

Type of sliding microtome recommended for cuttinh extremely hard and rough tissue blocks

Standard sliding microtome

Sections in the base-sledge microtome are cut in a perfectly _____ plane, thereby making excellent serial tissue sections

Flat

How many minutes does the cryostat freezes fresh tisssue?

within 2-3 mins

The specimen used in ultrathin microtome are _____, fixed in ______, and embedded in _______.

Small Osmium tetroxide Plastic

What is the thickness of the section in cryostat microtome?

4 u

Cryostat microtome consists of a microtome, usually a ______ microtome, keep inside in a cold chamber

Rotary

Type of sliding microtome used for cuttinh celloidin tissue blocks

Standard sliding microtome

What is the lenght of plane-concave knife?

25 mm

What is the freezing agent used in freezing microtome?

Liquid Carbon Dioxide

What is the lenght of biconcave knife?

100 mm

This a natural sharpening stone that removes nicks and irregularities on the knife edges

Hone

What is the direction in honing?

Heel to toe direction

It polish and sharpen the cutting edge

Stropping

What is the direction for stropping?

Toe to heel

How many strokes for honing?

10-20 strokes

Type of sliding microtome consist of two movable pillars holding the adjustable knife clamps, allowing the knife to be set at an angle for cutting celloidin sections

Base-sledge microtome

How many strokes for stropping?

40-120 strokes

What is the bevel angle?

27 to 32 degrees

A type of hone much coarse that is used for badly nicked knives

Fine carborundum

It involves the removal of gross nicks on the knife edge.

Honing

Type of hone that gives more polishing effect

Arkansas

A type of hone used for manual sharpening when cutting edge has been rendered blunt or nicked

Belgium yellow