STAINING

Question 1

Iron hematoxylin

Answer 1

• Weigert’s stain with Ferric chloride

Question 2

Active colorinh agent in hematoxylin

Answer 2

Hematin

Question 3

Alum hematoxylin stains (2)

Answer 3

• ehrlich’s soln w/ Na iodate

- harris’ soln with Mercuric chloride

Question 4

Hematoxylin : SN mexican tree

Answer 4

Hematoxylin Campechianum

Question 6

Oxidizing agents for hematoxylin

Answer 6

• H202
• Mercuric oxide
• Na iodate
• Na perborate
• K permanganate

Question 7

SN female cochineal bug

Answer 7

Coccus cacti

Question 8

Powerful chromatin and nuclear stain

Answer 8

Carmine dye

Question 9

Stains elastic fibers

Answer 9

Orcein

Question 10

Composition of orcein

Answer 10

vegetable dye (lichen) + ammonia (blue/violet)

Question 11

Biological stains

Answer 11

Natural dyes || Synthetic Dyes

Question 12

Oxidation process of hematin

Answer 12

Ripening

Question 14

dye classification (3)

Answer 14

acid dye
basic dye
neutral dye

Question 15

Carmine dye for Neuropathological studies

Answer 15

Picrocarmine

Question 16

Coal tar is derived from?

Answer 16

Hydrocarbon benzene

Question 17

Staining Reaction based on: (4)

Answer 17

1) Capillary osmosis
2) Solubility
3) Adsorption
4) Absorption

Question 17

Mordant used in hematoxylin

Answer 17

• Alum
• Iron
• Copper
• Chromium

Question 18

Spermatogenesis study

Answer 18

Copper hematoxylin

Question 18

Carmine dye with aluminum chloride for demonstration of GLYCOGEN

Answer 18

Best Carmine stain

Question 19

Fixation of basic dyes

Answer 19

• M. chloride

- Formaldehyde

Question 20

coal tar dyes

Answer 20

synthetic dyes

Question 21

Fxn of acid dyes

Answer 21

• chromium
• picric acid
• trichloroacetic acid

Question 22

regressive staining, diff. w/ 1% HCl in 70% OH

Answer 22

Ehrlich’s hematoxylin

Question 23

a basic dye which serves as an indicator and dye

Answer 23

methylene blue

Question 25

Routine nuclear staining, exfoliative cytology, sex chromosomes

Answer 25

Harris hematoxylin

Question 26

iron hematoxylin that demonstrates muscle fibers and connective tissue

Answer 26

weigert's

Question 27

auxilliary radical added to retain color

Answer 27

auxochrome

Question 27

fiserves as a fixative, decalcifying agent, and a softener

Answer 27

acid dyes

Question 28

neutral dye - leukocyte differentiation (2)

Answer 28

- giemsa stain | - leishman stain

Question 29

iron hematoxylin for cytological stain regressive staining of thin sections

Answer 29

heidenhain's

Question 30

routine counterstain, background contrasting stain

Answer 30

eosin

Question 31

Celestine blue hemalum method of nuclear staining

Answer 31

Mayer hematoxylin

Question 32

stains amyloid and platelets

Answer 32

crystal violet

Question 33

stains leukocyte nuclei

Answer 33

methylene violet

Question 34

counterstaining of epithelial sections

Answer 34

aniline blue

Question 35

nissl granules and chromophilic bodies

Answer 35

toluidine blue

Question 36

vital stain for circulatory system

Answer 36

prussian blue

Question 37

method used in staining elastic fibers using orcein

Answer 37

Taenzer unna orcein method

Question 38

stains parasite and bacteria

Answer 38

malachite green

Question 40

for blood smear

Answer 40

giemsa stain

Question 41

stains acid fast organism

Answer 41

basic fuchsin

Question 41

counterstain in papanicolaou method

Answer 41

bismarck brown

Question 42

stain for acid mucopolysaccharides

Answer 42

alcian blue

Question 43

embryos, elastic tissue, amyloid, and myelin

Answer 43

congo red

Question 44

specific for connective tissue and epithelial mucin

Answer 44

alcian blue

Question 45

cell granules and vacuoles of phagocytic cells

Answer 45

neutral red

Question 46

neuroglia (frozen section)

Answer 46

victoria blue

Question 47

discrimination of dead and living cells

Answer 47

acridine orange

Question 48

calcium deposits, sites phosphate activities

Answer 48

acridine red

Question 49

Method in embryos using congo red

Answer 49

Kraijan's method

Question 51

intravital staining of mitochondria

Answer 51

janus green B

Question 51

substitute for carbol fuchsin

Answer 51

night blue

Question 52

fat stains

Answer 52

lysochrome

Question 53

for hemoglobin

Answer 53

benzidine

Question 54

spirochete, reticulum and other fibers

Answer 54

silver nitrate

Question 55

griesbach method with osmic acid, blood, glandular tissue

Answer 55

rhodamine b

Question 56

stain solvents (4)

Answer 56

- distilled H20 - alcohol - aniline water - phenol

Question 57

fixative and fat stain in frozen section

Answer 57

osmium tetroxide

Question 58

stains phospholipids, TAG

Answer 58

sudan black

Question 60

using simple stain with aqueous or OH solution of dye

Answer 60

Direct staining

Question 61

stains TAG and no reaction to phospholipids and fine lipid

Answer 61

sudan IV

Question 61

stains CNS fats

Answer 61

sudan III

Question 62

staining with time sequence and no washing/decolorization

Answer 62

Progressive staining

Question 63

stain with mordant

Answer 63

Indirect staining

Question 64

accelerate or speed up staining reaction

Answer 64

accentuator

Question 65

overstaining and then decolorization

Answer 65

Regressive staining

Question 66

stain color reaction different from the color of ten stain itself

Answer 66

metachromasia

Question 67

using a different stain for contrast and background

Answer 67

counterstaining

Question 68

general differentiation of nucleus and cytoplasm

Answer 68

microanatomical staining

Question 69

using colorless soln of metallic salts

Answer 69

metallic impregnation

Question 70

selective staining of living cells

Answer 70

vital staining

Question 71

stain for RES

Answer 71

Trypan blue

Question 72

Best vital dye

Answer 72

neutral red

Question 73

injecting the dye before extraction of the tissue

Answer 73

intravital staining

Question 74

staining immediately after removal of the tissue from the body

Answer 74

supravital staining

Question 75

Variation in affinities for most dyes and stains

Answer 75

Differentiation