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[2ND YEAR] CCP 5.1 - Pharmaceutical Microbiology & Parasitology (Lab) > MIDTERM 01 - Culture Media > Flashcards

MIDTERM 01 - Culture Media Flashcards

1
Q

Refers to food materials or substances required for growing microorganisms in vitro (outside the body)

A

Culture media/Growth media

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2
Q

Refers to the growth of microorganisms

A

Culture

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3
Q

Media where agar is the most commonly used solidifying agent (Media classifications based on physical state)

A

Solid media

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4
Q

Is a golden-yellow granular powder prepared from seaweeds that is not affected by the growth of bacteria

A

Agar

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5
Q

Media with a lower agar concentration; useful in the cultivation of microaerophilic bacteria (Media classifications based on physical state)

A

Semi-solid media

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6
Q

Semi-solid media is useful in the cultivation of __________ bacteria

A

Microaerophilic

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7
Q

Media referred to as “broth”; bacteria grow uniformly producing general turbidity (Media classifications based on physical state)

A

Liquid media

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8
Q

2 examples of liquid media (NP)

A

Nutrient broth, Peptone solution

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9
Q

Only aerobic growth; oxygen is required (Types of bacteria according to oxygen requirement)

A

Obligate aerobes

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10
Q

Both aerobic and anaerobic growth; has greater growth in the presence of oxygen (Types of bacteria according to oxygen requirement)

A

Facultative anaerobes

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11
Q

Only anaerobic growth; growth ceases in the presence of oxygen (Types of bacteria according to oxygen requirement)

A

Obligate anaerobes

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12
Q

Only anaerobic growth, but growth continues in the presence of oxygen (Types of bacteria according to oxygen requirement)

A

Aerotolerant anaerobes

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13
Q

Only aerobic growth; oxygen required in low concentrations (Types of bacteria according to oxygen requirement)

A

Microaerophiles

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14
Q

Growth occurs only where high concentrations of oxygen have diffused into the medium (Types of bacteria according to oxygen requirement)

A

Obligate aerobes

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15
Q

Growth is best where most oxygen is present, but occurs throughout the tube (Types of bacteria according to oxygen requirement)

A

Facultative anaerobes

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16
Q

Growth occurs only where there is no oxygen (Types of bacteria according to oxygen requirement)

A

Obligate anaerobes

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17
Q

Growth occurs evenly; oxygen has no effect (Types of bacteria according to oxygen requirement)

A

Aerotolerant anaerobes

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18
Q

Growth occurs only where a low concentration of oxygen has diffused into the medium (Types of bacteria according to oxygen requirement)

A

Microaerophiles

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19
Q

A container into which a lit candle is introduced before sealing the container’s airtight lid

A

Candle jar

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20
Q

General purpose media that supports growth of non-fastidious microbes; primarily used for the isolation of microorganisms (Media classifications based on ingredients)

A

Simple media (Basal media)

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21
Q

2 examples of simple media/basal media (NN)

A

Nutrient broth, Nutrient agar

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22
Q

Media that has ingredients wherein the exact components are difficult to estimate (Media classifications based on ingredients)

A

Complex media

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23
Q

Specifically prepared media from pure chemical substances used for research purposes; the composition of every component is well known (Media classifications based on ingredients)

A

Synthetic/Defined media

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24
Q

Example of synthetic/defined media

A

Peptone water

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25
Substances like blood, serum, and egg are added to the simple medium; used to grow bacteria that are exacting in their nutritional needs (Types of special media)
Enriched media
26
Example of fastidious microorganism
Neisseria gonorrhoeae
27
2 examples of enriched media (BC)
Blood agar, Chocolate agar
28
Agar that contains mammalian blood used to isolate fastidious organisms and detect hemolysis (Examples of enriched media)
Blood agar
29
Agar that contains red blood cells that have been lysed; used for growing fastidious bacteria (Examples of enriched media)
Chocolate agar
30
Shows complete lysis of red blood cells resulting in complete clearing around the colonies (Types of Streptococci hemolysis)
Beta hemolysis
31
Shows incomplete lysis of red blood cells, producing a greenish discoloration of the blood agar around the colonies (Types of Streptococci hemolysis)
Alpha hemolysis
32
No hemolysis; results in no change in the medium (Types of Streptococci hemolysis)
Gamma hemolysis
33
An inhibitory substance is added to a solid media to inhibit commensal or contaminating bacteria such as antibiotics, dyes, chemicals, and alteration of pH (Types of special media)
Selective media
34
Media used selectively for Neisseria gonorrhoeae (Examples of selective media)
Thayer Martin media
35
Antibiotic that is able to kill most gram positive organisms (Antibiotics found in Thayer Martin media)
Vancomycin
36
Antibiotic that is added to kill most gram negative organisms except Neisseria (Antibiotics found in Thayer Martin media)
Colistin
37
Can kill most fungi (Antibiotics found in Thayer Martin media)
Nystatin
38
Inhibits gram negative organisms, especially swarming Proteus (Antibiotics found in Thayer Martin media)
Trimethoprim
39
Media that is selective for gram negative bacteria; the dye methylene blue in the media inhibit the growth of gram positive bacteria (Examples of selective media)
Eosin methylene blue (EMB) media
40
Media that is used for the isolation of Camphylobacter jejuni from fecal or rectal swabs; contains bacteriological charcoal, cefoperazone, and amphotericin B (Examples of selective media)
Camphylobacter agar
41
3 components of Camphylobacter agar (BAC)
Bacteriological charcoal, Amphotericin B, Cefoperazone
42
Is a solid media used for Mycobacterium tuberculosis; contains penicillin, nalidixic acid, and malachite green (Examples of selective media)
Lowenstein-Jensen media
43
3 components of Lowenstein-Jensen media (PNM)
Penicillin, Nalidixic acid, Malachite green
44
Media that are designed in such a way that different bacteria can be recognized on the basis of their colony color (Types of special media)
Differential media
45
Media that distinguishes between lactose fermenters and non lactose fermenters (Types of differential media)
MacConkey media
46
Lactose fermenters produce __________ colonies
Pink
47
Non lactose fermenters produce __________ colonies
Colorless
48
Media that distinguished between mannitol and non mannitol fermenters (Types of differential media)
Mannitol salt agar
49
Mannitol fermenters include __________
Staphylococcus aureus
50
Non-mannitol fermenters include __________
Staphylococcus epidermidis
51
Positive growth but non-mannitol fermenters include __________
Micrococcus luteus
52
Negative growth includes __________ and ___________ (EP)
Escherichia coli, Pseudomonas aeruginosa
53
Media used for the isolation of Salmonella and Shigella spp. from clinical specimens and food samples (Types of differential media)
Xylose lysine deoxycholate (XLD) agar
54
Media used for cultivation of pathogen from urine specimen and to inhibit swarming of Proteus sp. (Types of differential media)
Cysteine lactose electrolyte deficient (CLED) agar
55
Media that is highly selective for the isolation of Vibrio cholerae and Vibrio parahaemolyticus (Types of differential media)
Thiosulfate-citrate-bile salts-sucrose (TCBS) agar
56
Media used for transporting the samples (Types of special media)
Transport media
57
2 examples of transport media (SB)
Stuart's media, Buffered glycerol saline
58
These media are used to grow anaerobic organisms (Types of special media)
Anaerobic media
59
2 examples of anaerobic media (RT)
Robertson's cooked meat media, Thioglycolate broth media
60
Agar used for isolation and cultivation of fungi
Sabouraud dextrose agar (SDA)
61
Agar used to promote growth and differentiation of plant cells
Marushige and Skoog medium (MSM)
62
Agar used for plant cell suspension and callus cultures
Gamborg's B5 medium

[2ND YEAR] CCP 5.1 - Pharmaceutical Microbiology & Parasitology (Lab) (8 decks)

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