MISCELLANEOUS TESTS FOR YEAST IDENTIFICATION

Question 1

• most important and easiest to perform for yeast identification
• uses serum/plasma (fetal bovine serum), expired FFP from blood bank

Answer 1

Germ Tube Production

Question 2

T/F
C. albicans – no grow at 42 degC
C. dubliniensis – can grow at 42 degC

Answer 2

FALSE
C. albicans – CAN grow at 42 degC
C. dubliniensis – NO grow at 42 degC

Question 3

T/F
Pseudogerm tube – has constriction at base (C. Tropicalis)

Answer 3

TRUE

Question 4

• identifies which carbohydrate a yeast can use aerobically API 20C Yeast ID System: most commonly used
• assimilated sugars: wells become turbid with growth

Answer 4

Carbohydrate Assimilation

Question 5

identifies based on colony colors

Answer 5

CHROMagar Candida

Question 6

• 2nd in importance to sugar assimilation in yeast ID
• used to elicit chlamydoconidia production of C. albicans

Answer 6

Cornmeal Agar