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MIDTERMS > MLSP: Microbiology > Flashcards

MLSP: Microbiology Flashcards

1
Q

Allow room air to pass into
cabinet and around the area and
material within, sterilizing only the
air to be exhausted

A

CLASS I

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2
Q

Consist of negative pressure and operated in open front

A

CLASS I

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3
Q

Air flows in “sheets”, which serves
as a barrier to particle from outside
the cabinet

A

CLASS II CLASS II

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4
Q

Direct the flow of contaminated
air into the filters, also called as VERTICAL LAMINAR FLOW BSCs

A

CLASS II

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5
Q

Self-contained, and 70% of the air
is recirculated [most common in
clinical microbiology section]

A

CLASS IIA

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6
Q

Selected for radioisotopes, toxic
chemicals or carcinogenic samples

A

CLASS IIB

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7
Q

Prokaryotic unicellular organisms that lack a true nucleus and nuclear membrane

A

BACTERIA

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8
Q

Single, closed, circular chromosomes of double-
stranded DNA called?

A

NUCLEOID

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9
Q

small circular molecules o
extrachromosomal circular DNA

A

PLASMIDS

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10
Q

May either be GRAM POSITIVE [violet] or GRAM
NEGATIVE [pink or reddish pink]

A

BACTERIA

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11
Q

asexual reproduction of bacteria

A

BINARY FISSION

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12
Q

range from 0.2 to 2um in diameter
and 1 to 6um in length

A

Bacteria size

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13
Q

Principal stain used for microscopic examination of bacteria

A

GRAM STAIN

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14
Q

T or F
Gram stain is first devised by Hans Christian Gram during the late 19th century

A

True

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15
Q

take up the basic dye, crystal violet

A

Gram-positive

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16
Q

allow crystal violet dye to wash out easily with decolorizer alcohol or acetone

A

Gram-negative

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17
Q

CELL WALL
Thick (peptidoglycan)

A

Gram positive

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18
Q

CELL WALL
Thin (peptidoglycan)

A

Gram negative

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19
Q

LIPOPOLYSACCHARIDE
Absence

A

Gram positive

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20
Q

LIPOPOLYSACCHARIDE
Presence

A

Gram negative

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21
Q

GRAM STAINING
Purple

A

Gram positive

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22
Q

GRAM STAINING
Red or Pink

A

Gram negative

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23
Q

REPRESENTATION
Purple or Violet result

A

Gram positive

24
Q

REPRESENTATION
Pink result

A

Gram negative

25
Q

spherical-shaped cells

A

COCCI

26
Q

rod-shaped cells

A

BACILLI

27
Q

spiral shaped cells

A

SPIRILLA

28
Q

comma-shaped cells

A

VIBRIOS

29
Q

T or F
FOUR BASIC MORPHOLOGICAL TYPES OF BACTERIA; COCCI, BACILLI, SPIRILLA, VIBRIOS

A

True

30
Q

Process by which bacteria catabolize carbohydrates to produce energy

A

FERMENTATION

31
Q

Also known as oxidation. Process of bacterial energy generation rather than fermentation

A

RESPIRATION

32
Q

Tongue should be depressed before swabbing
between the tonsillar pillars and behind the uvula

A

THROAT (SWABS)

33
Q

The cheek, tongue, and teeth should NOT be
touched

A

THROAT (SWABS)

34
Q

Expectorated specimens from deep cough should
be collected into a sterile specimen cup

A

SPUTUM

35
Q

Should never be taken from the toilet and should not be contaminated with urine

A

STOOL

36
Q

Midstream clean-catch is the most common
collection method

A

URINE

37
Q

Culture for catheterized urine specimens usually have less contaminating bacterial flora

A

URINE

38
Q

2-3 cultures should be collected at random times during 24-hour period

A

BLOOD

39
Q

20-30mL of blood per culture is collected

A

ADULT

40
Q

1-5mL of blood per culture is collected

A

INFANTS

41
Q

Should be collected aseptically by PHYSICIAN

A

CEREBROSPINAL FLUID

42
Q

Should be processed immediately and not exposed to heat or refrigeration

A

CEREBROSPINAL FLUID

43
Q

Exudates may be expressed from the urethral
orifice or a small-diameter swab inserted 3-4
cm into the urethra

A

MEN (penile discharge)

44
Q

A sterile swab is inserted into the cervix with an
aid of speculum, swab is rotated and allowed to remain for a few seconds

A

WOMEN (vaginal discharge)

45
Q

Anaerobic specimens must be transported in an anaerobic system

A

HANDLING AND TRANSPORT

46
Q

T or F
Aerobic set-up is okay to be exposed in oxygen

A

True

47
Q

T or F
Anaerobic should not be exposed to oxygen

A

True

48
Q

T or F
Swab samples are not allowed to dry out

A

True

49
Q

T or F
Most specimen can be held at 2°C to 8°C

A

True

50
Q

Support the growth of a wide range of
microorganisms and are considered non
selective because, the growth of most
organisms is supported

Example: Blood Agar Plate and Chocolate Agar

A

Nutritive Media

51
Q

Microorganisms that can be distinguished on
the basis if certain growth characteristics
evident on the medium

Example: Blood Agar Plate

A

Differential Media

52
Q

Support the growth of one group of organisms,
but not another, by adding antimicrobials, dyes,
or alcohol to a particular medium

Example: MacConkey Agar [only allow gram-
negative organisms], Columbia agar with
colistin and nalidixic acid (CAN) Agar

A

Selective Media

53
Q

Also called supplemental or enrichment broth
For detection of small numbers of organisms
present

Example: Thioglycollate (Thio) broth, brain-
heart infusion broth (BHIB) and tryptic soy
broth (TSB)

A

Backup Broth

54
Q

Used when specific organisms are suspected

Example: Mannitol salt agar, bismuth sulfite
agar, Campylobacter agar, Thiosulfate-citrate-
bile salts-sucrose (TCBS) agar

A

Specialized Media

55
Q

INCUBATION
35°C-37°C

A

Normal incubation temperature

56
Q

INCUBATION
42°C to 45°C

A

for stool cultures detection of
Campylobacter jejuni

57
Q

T or F

Anaerobic jars, bags, or an anaerobic chamber are appropriate for incubation of anaerobic cultures

A

True

MIDTERMS (21 decks)

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