Module 4 Flashcards
The diazonium salt used in bilirubin reagent strip is either
✓ dichloroaniline (most common) or;
✓ dichlorobenzene-diazonium- tetrafluoroborate
Reagent strip test for urobilinogen is based on a modified
Ehrlich reaction between ____and ____ in a strongly acidic medium to produce
a pink to red color.
p-dimethyl aminobenzaldehyde
and urobilinogen
rapid, indirect method for the detection of
significant & asymptomatic bacteriuria.
NITRITE TEST
In an
acidic medium, nitrite in the urine reacts with_____
to form a diazonium compound.
p- arsanilic acid
The diazonium compound then
couples with quinoline compound _____to
produce a pink color.
1-naphthyl-ethylenediamine
Neutrophils contain enzymes
known as ____
esterases.
In leukocyte esterase pyrazole reacts with diazonium salt to produce ___
beige pink to purple color
Reagent strips use a _____that measure urine pH between pH 5 to 9.
Double indicator system of methyl red and bromthymol blue
color change of METHY RED at pH 4-6
RED-ORANGE TO YELLOW
BROMTHYMOL BLUE
YELLOW TO GREEN TO BLUE
longest reading time in a reagent strip
leukocyte esterase
Reading time for LE
2 minutes
Reading time for bilirubin
30 seconds
Reading time for glucose
30 secs
Reading time for ketone; SG
40 secs; 45 secs
What will happen to the urine specific gravity of patients with urinary tract infection
INCREASE/DECREASE?
increased
What will happen to the urine specific gravity of a college student after drinking alcohol at a party?/ INC or DEC?
Decreased
What will happen when excess urine is left on reagent strip after removing it from the specimen?
a. Cause run-over effect
b.Alter the color of the specimen
c. Cause reagents to leach from the pads
d. Not affect the chemical reactions
a. Cause run-over effect
Quality control of reagent strips is performed
a. Using positive and negative controls
b. when results are questionable
c. at least once every 24 hrs
d. All of the above
d. All of the above
Employs the principle of Greiss reaction
Nitrite
Which of the followingis the principle of protein-high pad on the reagent strip
a. Protein error of indicators
b. Greiss reaction
c.Dissociation of polyelectrolyte
d. Double indicator reaction
Presence of bence jones protein may indicate which of the ff conditions?
a. microalbuminuria
b. multiple myeloma
c. diabetic nephropathy
d. Lupus erythromatosus
b. multiple myeloma
At which temperatures does bence jones protein coagulate?
between 40 and 60n degrees celsius
Qualitative test for proteins in urine includes all of the following except
a.SSA
b. Esbach’s Test
c. Heller’s Tesy
d. Harrison’s Test
Esbach’s test
