Module 4

Question 1

A generic term to include the in vitro cultivation of organs, tissues, and cells

Answer 1

Tissue culture

Question 2

A spherical or 3D shape; specific histological interaction

Answer 2

Organ culture

Question 3

• A fragment of tissue
• Attachment and migration occurs in the plant of the solid substrate

Answer 3

Primary explant culture

Question 4

• Adherent monolayer on solid substrate (various cell type)
• Suspension in the culture medium (new cell types)

Answer 4

Cell culture

Question 5

Compare animals’ cells and microbial cells

Answer 5

Animal cells are more complex while microbial cells are simpler, In growth rate AC are slower compared to MC, in sensitivity to shears AC is more fragile while MC are robust, when it comes to nutritional requirements AC are more complex and not well defines and also expensive while MC are simpler a less expensive.

Question 5

3 types of Morphology of cells in animal cells

Answer 5

Fibroblast-like, Epithelial-like and Lymphoblast-like

Question 6

Types of culture

Answer 6

• suspension culture
• Adherent (monolayer)

Question 7

Bipolar or multipolar
-Elongated shapes
- Grow attached to a substrate

Answer 7

Fibroblast-like cells