Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

HEMA BLOOD CELL ANOMALIES > MOLBIO > Flashcards

MOLBIO Flashcards

(62 cards)

Start Studying
1
Q

The branch of biology that studies the structure, composition, and interactions of cellular biomolecules

A

Molecular biology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Deals with molecular techniques that are designed for handling and analysis of nucleic acids
- it is rooted in the understanding of genome structure and cellular activities at the molecular level

A

Diagnostic Molecular Biology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q
  • accurate and error free
  • total process guaranteeing accuracy of final results reported
  • systemic measurement , comparison with a standard
A

QUALITY ASSURANCE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

State the 3 Importance of quality assurance

A
  1. Results impact management
  2. Laboratory faces issues and challenges in daily operations
  3. Most critical quality issue in the laboratory is contamination
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Contamination sources:

A
  1. Environment
  2. Other specimen and amplicans
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

These are products of amplification

A

Amplicans

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the contamination effects?

A

False negative results
False positive results

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the five components considered in MDBX

A
  1. Personnel requirements
  2. Facilities and workflow
  3. Assay requirements
  4. External quality assurance program
  5. Quality assurance
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

True or false:
staffs must undergo general orientations and trainings

A

FALSE: New hires must undergo general orientations and trainings

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

PERSONNEL REQUIREMENTS:
Staff must display____ and display evidence of ____

A

Proficiency testing and proficiency

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

TRUE OR FALSE: Personnel requirements must satisfy quantity requirements

A

Quality requirements

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Procedure performed in different rooms
- separation of work areas/unidirectional workflow

A

SPACE AND TIME PREPARATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

UNIDIRECTIONAL WORKFLOW SEQUENCE

A

Reagent > sample> amplification > post amplification

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Clean rooms have? And dirty rooms have?

A

Clean rooms: positive air pressure
Dirty rooms: negative air pressure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What are the laboratory practices?

A
  • use of appropriate PPE in the performance of laboratory tasks
  • decontamination in procedures for staff, work areas, equipment supplies
  • employ techniques that eliminate contamination
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Chemical control:
True of false:
Surface decontamination by 10% alcohol and 70% bleach solution

A

TRUE OR FALSE:
Surface decontamination by 70% alcohol and 10% bleach solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Chemical control:

A
  • Surface decontamintaion by 70% alcohol and 10% bleach solution
  • usage of bleach-based decontaminating agents
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

ENVIRONMENTAL CONTROL:

A
  1. Airflow must be considered
  2. Avoid unnecessary movements/ activities by the staff
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What are the 4 components under facilities and workflow?

A
  1. Space and time preparation
  2. Laboratory practices
  3. Chemical control
  4. Environmental control
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What are the 3 assay requirements?

A
  1. Clinical specimen
  2. Reagents, control and standards
  3. Equipment maintenance
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Specimen requirements (_____, _____, and ______) must be allowed

A

Type, volume and collection procedure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Proper transport and storage procedures

A

Clinical specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Acquire supplies from reliable sources

A

Reagents, control and standards

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Relevant details of supplies should be noted and documented

A

Regents, control and standards

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Performance of supplies should be monitored
Reagents, control, and standards
26
Must be acquired from reliable sources:
Reagents, control, and standards Equipment maintenance
27
Usage should be logged
Equipment maintenance
28
Onsite calibration/ validation and regular preventive maintain should be on schedule
Equipment maintenance
29
Evaluation of laboratory’s performance on examination of samples of external origin for the purpose of examining the lab’s pre- exam examination and post examination
External quality assurance program
30
What are the components under the external quality assurance program
1. Proficiency testing 2. Concordance testing 3. Laboratory assessment 4. Laboratory staff competency assessment 5. Simple referral and turn around time
31
Set of procedures undertaken by the staff for continuously assessing laboratory work and the emergent results in order to decide whether results are reliable enough to be continued
Quality assurance
32
Measures that must be included during each assay run to verify that the test is working properly
Quality assurance
33
2 use of assay controls
1. Extraction control 2. PCR control
34
Name the 4 controls under the PCR control
Internal control Positive control Negative control No template control
35
Containment principles, technologies, and principles implemented to prevent unintentional exposure to pathogens and toxins and their unintentional release
Laboratory biosafety
36
Protection, control and accountability for valuable biological materials within laboratories in order to prevent their: Unauthorized access Loss Theft Misuse diversion Unintentional release
Laboratory biosecurity
37
System or process to control safety and security risks associated with the handling or storage and disposal of biological agents and toxins in laboratories and facilities
Laboratory biorisk management
38
What are the major steps in laboratory risk management
Assessment Mitigation Performance
39
What are the detailed steps jn the assessment of laboratory risk management
Risk identification Hazard/ threat identification Likelihood evaluation Consequences evaluation
40
Mitigation detailed steps
Biorisk control measures Risk management
41
Perfromance detailed steps
Control Assurance Improvement
42
Give the Three letter symbol and one letter symbol Alanine
Three letter symbol: Ala One letter symbol: A
43
Arginine
Arg R
44
Asparagine
Asn N
45
Aspartic acid
Asp D
46
Cysteine
Cys C
47
Glutamic acid
Glu E
48
Glutamine
Gln Q
49
Histidine
His H
50
Isoleucine
Ile I
51
Leucine
Leu L
52
Lysine
Lys K
53
Methionine
Met M
54
Phenylalanine
Phef
55
Proline
Pro P
56
Serine
Ser S
57
Threonine
Thr T
58
Tryptophan
Trp W
59
Tyrosine
Tyr Y
60
Valine
Val V
61
Start codon
AUG
62
Stop codon
AUU UAG UGA

HEMA BLOOD CELL ANOMALIES (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions