Molecular genetics part 2 Flashcards Preview

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Flashcards in Molecular genetics part 2 Deck (28)
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1

DNA is found in the _____ of the cell

Nucleus

2

What is located in the cytoplasm of the cell?

tRNA

3

tRNA:

Is responsible for picking up amino acids and aligning them in the order specified by the gene

4

On which strand is the anti-codon found?

tRNA

5

Which strand is the codon found?

mRNA

6

Polypeptide chain:

Are amino acids linked together in long chains

7

Long chains of amino acids contain many ____bonds

peptide bonds

8

Bases found before or after the start and stop codon on the mRNA are called?

Introns

9

Which part of the cell does transcription occur?

Nucleus

10

Translation occurs in the ____ of the cell.

Cytoplasm

11

What occurs in termination?

The stop codon is reached and translation stops

12

Initiation:

The start codon AUG begins the translation process

13

What is responsible for reading the mRNA base sequence?

tRNA

14

What is the consequence of changing the codon UGA to UGG?

The protein will be longer than normal

15

Mendel's three laws:

1. Law of dominance
2. Law of segregation
3. Law of independent assortment

16

Law of dominance:

Some traits are stronger (dominant) and will hide weaker recessive traits.

17

Law of segregation:

Traits are controlled by a pair of genes. These factors that occur in pairs, are separated from each other during gamete formation and recombined during fertilization

18

Law of independent assortment

The inheritance of alleles from one trait does not affect the inheritance of alleles for another trait.

19

In the bacterial transformation lab, what was the source of the glowing?

A protein inside the E. Coli

20

Why do scientists use restriction enzymes?

To cut DNA at a specific point in a sequence of bases

21

What was the role of arabinose sugar in the bacterial transformation lab?

It was the inducer molecule that turned on the operon

22

Operon:

- Is a gene that can be 'turned on' or 'turned off' when needed
- Can be regulated by the presence of a specific molecule

23

What gene from the plasmid used in the bacterial transformation lab codes for ampicillin resistance?

BLA

24

Which gene from the plasmid used in the bacterial transformation lab acts as the operon?

araC

25

What slowed the movement of the plasma membrane in the bacterial transformation lab?

Ice

26

What neutralized the negative charge of the plasma membrane in the bacterial transformation lab?

CaCl2

27

When creating recombinant DNA using ligation, which enzyme catalyzes the joining of two DNA fragments?

DNA ligase

28

Annealing

Uses sticky ends and complimentary base pairing attraction when creating recombinant DNA.